Sox17 mediates adult arterial endothelial cell adaptation to hemodynamics

SOX17/ETV2 improves the direct reprogramming of adult fibroblasts to endothelial cells

“…ETV2 is well established as a master transcription factor for EC fate, 5 and SOX17 helps improve the engraftment of newly formed blood vessels 20 and drives an arterial phenotype in ECs. 14 Together, these transcription factors generate more mature rECs at a higher efficiency than ETV2 alone. The ETV2-ECs performed very similarly to those established in previous works.…”

“…Additionally, iPSC-ECs can be directed toward an arterial phenotype by culturing them with VEGF and cAMP, 35 similar to how SOX17 can be used to drive arterial EC transdifferentiation. 14 It would be interesting to see if these small molecules play a role in determining arteriovenous identity in transdifferentiation or even make the overall reprogramming process more efficient. However, the main difference between iPSC-ECs and rECs is the expression of eNOS.…”

“… 12 , 13 Additionally, overexpressing SOX17 in venous ECs drives them to an arterial phenotype, and they begin to increase the expression of genes relating to arterial and tip cell identity. 14 SOX17 has other roles in the mesoderm, including hematopoiesis, 15 , 16 endocardium and cardiomyocyte differentiation, 17 , 18 and fibroblast-to-EC transdifferentiation. 19 Schachterle et al.…”

Manufactured tissue-to-tissue barrier chip for modeling the human blood–brain barrier and regulation of cellular trafficking

“…4 While PDMS has been widely used thanks to the biocompatibility, optical transparency, and gas permeability, 5 it has also been known to be limited to a lab material, not for standardized models in preclinical drug testing and translation, due to apparently critical disadvantages including selective absorption of various molecules [6][7][8] and, particularly, difficulty in mass production. 9 To facilitate mass production of MPS, plastic materials such as polycarbonate, 10 polystyrene, 11 cyclic olefin (co) polymer [12][13][14] have been used with conventional mechanical approaches including laser cutting, micro-milling, and injection molding. Injection molding has been preferably utilized in commercialized MPS with high scalability, good transparency, and minimal molecular absorption.…”

VEGF counteracts shear stress-determined arterial fate specification during capillary remodeling

“…Besides VEGF, blood flow-derived fluid shear stress (FSS) has also been shown to induce Notch and arterial specification [15][16][17] . Notably, FSS-induced cell cycle G1/S arrest is reported as a pre-requisite for arterial fate acquisition 15 .…”