Manufactured tissue-to-tissue barrier chip for modeling the human blood–brain barrier and regulation of cellular trafficking

Kim, Jae-Hoon; Yoon, Taehee; Kim, Paul; Bekhbat, Mandakh; Kang, Seung-Gul; Rho, Hoon Suk; Ahn, Song Ih; Kim, Yong Tae

doi:10.1039/d3lc00124e

Cited by 6 publications

(4 citation statements)

References 56 publications

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“…[ 199 , 203 ] This latter MEPS‐TBC model is composed of endothelial cells lining blood vessels under blood flow, pericytes wrapping around the vessel, and astrocyte end feet in contact with the blood vessel. [ 204 ] These innovative platforms addressing the complexity of physiological environment and dynamic simulation are highly recommended in future studies, which may offer alternatives for investigating BBB permeability of pharmaceuticals and NDC, and further enable a better understanding of the NPs delivery mechanisms involved in BBB.…”

Section: Novel Physiological Models Mimicking the In Vivo Np Cellular...mentioning

confidence: 99%

Bridging Smart Nanosystems with Clinically Relevant Models and Advanced Imaging for Precision Drug Delivery

Zhou,

Liu,

Wang

et al. 2024

Advanced Science

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Intracellular delivery of nano‐drug‐carriers (NDC) to specific cells, diseased regions, or solid tumors has entered the era of precision medicine that requires systematic knowledge of nano‐biological interactions from multidisciplinary perspectives. To this end, this review first provides an overview of membrane‐disruption methods such as electroporation, sonoporation, photoporation, microfluidic delivery, and microinjection with the merits of high‐throughput and enhanced efficiency for in vitro NDC delivery. The impact of NDC characteristics including particle size, shape, charge, hydrophobicity, and elasticity on cellular uptake are elaborated and several types of NDC systems aiming for hierarchical targeting and delivery in vivo are reviewed. Emerging in vitro or ex vivo human/animal‐derived pathophysiological models are further explored and highly recommended for use in NDC studies since they might mimic in vivo delivery features and fill the translational gaps from animals to humans. The exploration of modern microscopy techniques for precise nanoparticle (NP) tracking at the cellular, organ, and organismal levels informs the tailored development of NDCs for in vivo application and clinical translation. Overall, the review integrates the latest insights into smart nanosystem engineering, physiological models, imaging‐based validation tools, all directed towards enhancing the precise and efficient intracellular delivery of NDCs.

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Section: Novel Physiological Models Mimicking the In Vivo Np Cellular...mentioning

confidence: 99%

Bridging Smart Nanosystems with Clinically Relevant Models and Advanced Imaging for Precision Drug Delivery

Zhou,

Liu,

Wang

et al. 2024

Advanced Science

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“…Hybrid designs, combining porous membrane-based and hydrogel-based features, are also possible. 38 In such cases, a hydrogel-based chip could be a lower part of the conventional porous membrane-based chip (Fig. 1C).…”

Section: Key Design Parameters To Develop Functional Tissue Barrier Chipmentioning

confidence: 99%

Reconstitution of human tissue barrier function for precision and personalized medicine

Kim,

Yoon,

Lee

et al. 2024

Lab Chip

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“…A most promising modification of the tridimensional culture assays of special interest for drug screening, is the so-called “organ-on-a-chip” ( 41 ). In this assay, cells are cultured in micro-channels subjected to controlled fluid flow within a microfluidic device that is provided with biosensors to monitor biomarkers secreted by the organoids ( 42 , 43 ). In addition to detect modifications induced by selected drug treatments, this assay enables to explore interactions between distinct organoids growing within channels interconnected together (“multiorgans microdevice”) or to generate a network of microvessels by adding growing vascular progenitors in connection with the organoid ( 44 ).…”

Section: Introductionmentioning

confidence: 99%

“…In addition to detect modifications induced by selected drug treatments, this assay enables to explore interactions between distinct organoids growing within channels interconnected together (“multiorgans microdevice”) or to generate a network of microvessels by adding growing vascular progenitors in connection with the organoid ( 44 ). Over-all, the organ-on-chip technology is a fast-moving field of research, and we could anticipate, that distinct types of these organ chip models will be manufactured and standardized in the next future [see ( 42 , 45 )].…”

Section: Introductionmentioning

confidence: 99%

Advances in the knowledge and therapeutics of schizophrenia, major depression disorder, and bipolar disorder from human brain organoid research

Villanueva

2023

Front. Psychiatry

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Tridimensional cultures of human induced pluripotent cells (iPSCs) experimentally directed to neural differentiation, termed “brain organoids” are now employed as an in vitro assay that recapitulates early developmental stages of nervous tissue differentiation. Technical progress in culture methodology enabled the generation of regionally specialized organoids with structural and neurochemical characters of distinct encephalic regions. The technical process of organoid elaboration is undergoing progressively implementation, but current robustness of the assay has attracted the attention of psychiatric research to substitute/complement animal experimentation for analyzing the pathophysiology of psychiatric disorders. Numerous morphological, structural, molecular and functional insights of psychiatric disorders have been uncovered by comparing brain organoids made with iPSCs obtained from control healthy subjects and psychiatric patients. Brain organoids were also employed for analyzing the response to conventional treatments, to search for new drugs, and to anticipate the therapeutic response of individual patients in a personalized manner. In this review, we gather data obtained by studying cerebral organoids made from iPSCs of patients of the three most frequent serious psychiatric disorders: schizophrenia, major depression disorder, and bipolar disorder. Among the data obtained in these studies, we emphasize: (i) that the origin of these pathologies takes place in the stages of embryonic development; (ii) the existence of shared molecular pathogenic aspects among patients of the three distinct disorders; (iii) the occurrence of molecular differences between patients bearing the same disorder, and (iv) that functional alterations can be activated or aggravated by environmental signals in patients bearing genetic risk for these disorders.

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Manufactured tissue-to-tissue barrier chip for modeling the human blood–brain barrier and regulation of cellular trafficking

Abstract: Microphysiological system or organ-on-a-chip technologies can replicate the key structure and function of 3D human tissues with higher reproducibility than less controllable 3D cell aggregate models, providing great potential to...

Cited by 6 publications

References 56 publications

Bridging Smart Nanosystems with Clinically Relevant Models and Advanced Imaging for Precision Drug Delivery

Bridging Smart Nanosystems with Clinically Relevant Models and Advanced Imaging for Precision Drug Delivery

Reconstitution of human tissue barrier function for precision and personalized medicine

Advances in the knowledge and therapeutics of schizophrenia, major depression disorder, and bipolar disorder from human brain organoid research

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