Sox1 acts through multiple independent pathways to promote neurogenesis

Kan, Lixin; Israsena, Nipan; Zhang, Zuoli; Hu, Min; Zhao, Li; Jalali, Ali; Sahni, Vibhu; Kessler, John A.

doi:10.1016/j.ydbio.2004.02.005

Cited by 152 publications

(137 citation statements)

References 42 publications

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“…We observed certain similarities between our results in monkeys and studies using nonprimate models with respect to transcription factor expression by adult SVZa progenitors. In particular, Emx2 and Sox2,3 were expressed by actively dividing sustained progenitors in monkey SVZa consistent with their previously reported expression in early multipotent progenitor/stem cells (Galli et al, 2002;Bylund et al, 2003;Graham et al, 2003;Ferri et al, 2004;Komitova and Eriksson, 2004), while Sox1 (but not Sox2,3) protein was predominantly localized in monkey neuronal progenitors consistent with its previously reported activity inducing neuronal commitment (Kan et al, 2004). At the same time, we noticed the following discrepancies between our results in monkeys and studies using nonprimate animal models.…”

Section: Discussionsupporting

confidence: 90%

Transcription factor protein expression patterns by neural or neuronal progenitor cells of adult monkey subventricular zone

Tonchev¹,

Yamashima²,

Sawamoto³

2006

Neuroscience

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Abstract-The anterior subventricular zone of the adult mammalian brain contains progenitor cells which are upregulated after cerebral ischemia. We have previously reported that while a part of the progenitors residing in adult monkey anterior subventricular zone travels to the olfactory bulb, many of these cells sustain location in the anterior subventricular zone for months after injury, exhibiting a phenotype of either neural or neuronal precursors. Here we show that ischemia increased the numbers of anterior subventricular zone progenitor cells expressing developmentally regulated transcription factors including Pax6 (paired-box 6), Emx2 (empty spiracles-homeobox 2), Sox 1-3 (sex determining region Y-box 1-3), Ngn1 (neurogenin 1), Dlx1,5 (distalless-homeobox 1,5), Olig1,3 (oligodendrocyte lineage gene 1,3) and Nkx2.2 (Nk-box 2.2), as compared with control brains. Analysis of transcription factor protein expression by sustained neural or neuronal precursors in anterior subventricular zone revealed that these two cell types were positive for characteristic sets of transcription factors. The proteins Pax6, Emx2, Sox2,3 and Olig1 were predominantly localized to dividing neural precursors while the factors Sox1, Ngn1, Dlx1,5, Olig2 and Nkx2.2 were mainly expressed by neuronal precursors. Further, differences between monkeys and non-primate mammals emerged, related to expression patterns of Pax6, Olig2 and Dlx2. Our results suggest that a complex network of developmental signals might be involved in the specification of primate progenitor cells.

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Section: Discussionsupporting

confidence: 90%

Transcription factor protein expression patterns by neural or neuronal progenitor cells of adult monkey subventricular zone

Tonchev¹,

Yamashima²,

Sawamoto³

2006

Neuroscience

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“…During murine ear development Sox2 expression depends on Notch signaling and is required for the acquisition of neural potential in the prosensory epithelium (52). Subsequently, for neurogenesis to proceed, Sox2 is shut off via the accumulation of proneural genes, such as Atoh (93)(94)(95)(96)(97). In contrast, Sox2 is maintained in Notch + support cells, where it inhibits hair cell differentiation by inhibiting the proneural basic helix-loop-helix gene Atoh1a (91).…”

Section: Notch Signaling Is Down-regulated Early In Lateral Line Hairmentioning

confidence: 99%

Gene-expression analysis of hair cell regeneration in the zebrafish lateral line

Jiang

Romero‐Carvajal

Haug

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

136

152

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Deafness caused by the terminal loss of inner ear hair cells is one of the most common sensory diseases. However, nonmammalian animals (e.g., birds, amphibians, and fish) regenerate damaged hair cells. To understand better the reasons underpinning such disparities in regeneration among vertebrates, we set out to define at high resolution the changes in gene expression associated with the regeneration of hair cells in the zebrafish lateral line. We performed RNA-Seq analyses on regenerating support cells purified by FACS. The resulting expression data were subjected to pathway enrichment analyses, and the differentially expressed genes were validated in vivo via whole-mount in situ hybridizations. We discovered that cell cycle regulators are expressed hours before the activation of Wnt/β-catenin signaling following hair cell death. We propose that Wnt/β-catenin signaling is not involved in regulating the onset of proliferation but governs proliferation at later stages of regeneration. In addition, and in marked contrast to mammals, our data clearly indicate that the Notch pathway is significantly down-regulated shortly after injury, thus uncovering a key difference between the zebrafish and mammalian responses to hair cell injury. Taken together, our findings lay the foundation for identifying differences in signaling pathway regulation that could be exploited as potential therapeutic targets to promote either sensory epithelium or hair cell regeneration in mammals.signaling pathway analysis | RNA sequencing | neuromast | Jak/Stat3 | cdkn1b

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“…Importantly, the Wnt/β-catenin pathway is active in the early developing ovary and inactive in the early developing testis (Chassot et al, 2008). Furthermore, a number of SOX proteins, such as SOX1, SOX2, SOX3, SOX7, SOX9 and SOX17 inhibit the canonical Wnt/β-catenin signaling in different models of chondrocyte, neuronal and intestinal differentiation; however the mechanism of their action is still unclear (Akiyama et al, 2004, Bastide et al, 2007, Kan et al, 2004, Mansukhani et al, 2005, Sinner et al, 2007, Takash et al, 2001, Zorn et al, 1999. Here we investigated whether SRY could inhibit the canonical Wnt/β-catenin pathway.…”

Section: Introductionmentioning

confidence: 99%

Human SRY inhibits β-catenin-mediated transcription

Bernard

Sim

Knower

et al. 2008

The International Journal of Biochemistry & Cell Biology

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In most mammals, sex is determined by the presence or absence of the SRY gene. SRY encodes a DNA binding HMG-box transcription factor which, during embryogenesis, is the initial trigger of testis differentiation from the bipotential gonad, yet its precise mode of function remains unclear. In ovarian development, R-spondin1 and Wnt4 act through the Wnt/β-catenin signaling pathway to regulate TCF-dependent expression of unknown target genes and repress testis development. Conversely, SRY may be necessary to prevent the development of ovaries by inhibiting the action of ovarian-determining genes. We hypothesize that SRY prevents Wnt/β-catenin signaling, thereby inhibiting ovarian development. In HEK293T cells, SRY repressed β-catenin-mediated TCFdependent gene activation in the presence of a specific GSK3β inhibitor or an activated β-catenin mutant, suggesting that SRY inhibits Wnt signaling at the level of β-catenin. Three SRY mutant proteins with nuclear localization defects, encoded by XY male-to-female patients, failed to inhibit β-catenin; surprisingly four SRY sex reversed mutants with defective DNA binding activity showed near wild-type SRY inhibitory activity. Moreover the potent transactivator SRY-VP16 fusion protein also showed wild-type SRY inhibitory activity. Thus SRY inhibition of β-catenin involves neither DNA binding nor transactivation functions of SRY. β-catenin and SRY interact in-vitro and SRY expression triggered β-catenin localization into specific nuclear bodies in NT2/D1 and Hela cells. We conclude that SRY inhibits β-catenin-mediated Wnt signaling by a novel nuclear function of SRY that could be important in sex determination.

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Sox1 acts through multiple independent pathways to promote neurogenesis

Cited by 152 publications

References 42 publications

Transcription factor protein expression patterns by neural or neuronal progenitor cells of adult monkey subventricular zone

Transcription factor protein expression patterns by neural or neuronal progenitor cells of adult monkey subventricular zone

Gene-expression analysis of hair cell regeneration in the zebrafish lateral line

Human SRY inhibits β-catenin-mediated transcription

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