Sows intramammarily inoculated with Escherichia coli at parturition: I Functional capacity of granulocytes in sows affected or non-affected by clinical mastitis

Österlundh, Ingrid; Hultén, Fredrik; Johannisson, Anders; Magnusson, Ulf

doi:10.1016/s0165-2427(02)00204-0

Cited by 24 publications

(20 citation statements)

References 26 publications

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“…Previously we have not been able to show an impaired function of granulocytes before inoculation, the cells crucial in bacterial clearance in the mammary gland, in the sows that developed clinical signs of mastitis [43]. This study on the events preceding the granulocyte response, revealed that the production of IL-1β, IL-6, IL-8, and TNF-α significantly increased in the inoculated mammary glands of sows that were clinically affected by intramammary infection with E. coli.…”

Section: Discussionmentioning

confidence: 86%

“…Twelve pregnant crossbred (Swedish Landrace × Yorkshire) primiparous sows were obtained from a commercial farm and used in the study [43]. Sows were obtained approximately 6 to 8 weeks before anticipated parturition.…”

Section: Animalsmentioning

confidence: 99%

“…The experimental model and categorization of sows have been described in detail previously [43]. In brief, each teat on the right side of mammary glands (inoculated glands) was inoculated with 0.5 mL of bacterial suspension (10 5 colony forming units (CFU)/mL) containing E. coli strain serotype O127 during the 24-h period before parturition.…”

Section: Experimental Model and Categorization Of Sowsmentioning

confidence: 99%

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Morphometric analysis of proinflammatory cytokines in mammary glands of sows suggests an association between clinical mastitis and local production of IL-1beta, IL-6 and TNF-alpha

et al. 2007

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-Twelve healthy primiparous sows received intramammary inoculation with Escherichia coli (serotype O127) during the 24-h period preceding parturition. Mammary gland biopsy samples were taken immediately before inoculation (0 h) and from the inoculated and the contralateral noninoculated glands 24 h after inoculation. The analyses of interleukin-1 beta (IL-1β), IL-6, IL-8, and tumor necrosis factor alpha (TNF-α) by immunohistochemistry revealed that the production of these proinflammatory cytokines significantly increased in the inoculated mammary glands of sows that developed clinical signs of mastitis (affected group, n = 4) 24 h after inoculation. This was also true for IL-8 in the inoculated mammary glands of sows that did not develop clinical signs of mastitis (nonaffected group, n = 8). Sows that developed clinical signs of mastitis displayed significantly lower constitutive production of IL-1β than did sows that remained clinically healthy. The data indicate that the development of clinical symptoms of coliform mastitis in the sow is associated with a locally increased proinflammatory cytokine production in response to intramammary E. coli infection.cytokine / immunohistochemistry / mastitis / pig / E. coli

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Section: Discussionmentioning

confidence: 86%

Section: Animalsmentioning

confidence: 99%

Section: Experimental Model and Categorization Of Sowsmentioning

confidence: 99%

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Morphometric analysis of proinflammatory cytokines in mammary glands of sows suggests an association between clinical mastitis and local production of IL-1beta, IL-6 and TNF-alpha

et al. 2007

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“…Em leitões lactentes, o pH decresce com a produção de ácido láctico e somente bactérias resistentes ao ambiente ácido persistem e se proliferam no intestino proximal, ocasionando maior proteção contra a penetração de patógenos sensíveis ao meio ácido (19) . Embora tenha sido relatada a presença de E. coli no intestino de leitões duas horas após o nascimento, normalmente a análise histológica de amostras de intestino de animais afetados pelas toxinas termolábil (LT) e termoestável (ST) produzidas por cepas de E. coli não mostra lesões (20) , e a mucosa do intestino delgado apresenta-se sem alterações (19) . A microbiota do intestino delgado do leitão é dominada por espécies aeróbias e anaeróbias facultativas (3) .…”

Section: Resultsunclassified

Uso De Óleos Essenciais No Controle Dos Sinais Clínicos Das Diarreias Neonatais Em Leitões Nascidos De Fêmeas Com Diferentes Ordens De Parto

et al. 2015

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Resumo:Este estudo avaliou a emulsão líquida de óleos essenciais de orégano (Origanum vulgare) e alecrim (Rosmarinus officinalis labiatae) no controle dos sinais clínicos da diarreia neonatal em leitões. Foram monitorados 1.334 leitões lactentes, com peso vivo médio inicial de 1,5kg, quanto aos sinais clínicos de diarreia neonatal. O delineamento experimental foi o inteiramente casualizado e com três tratamentos (T): (T1) controle (solução fisiológica), (T2) mescla dos óleos essenciais e (T3) enrofloxacina. Os animais que receberam o T1 apresentaram sinais clínicos característicos de diarreia diferindo (P<0,01) dos demais tratamentos. O percentual de cura da diarreia dos leitões não diferiu (P>0,01) entre os tratamentos, independente da ordem de partos (OP) das mães. Já leitões com sinais clínicos de diarreia, nascidos de fêmeas com OP inferior a três apresentaram maior percentual de cura (74,6%), se comparados aos leitões diarreicos de fêmeas com OP entre três e cinco (68,2%) e superior a cinco (56,4%). As análises microbiológicas das fezes e histológicas do epitélio ileal não apresentaram diferenças (P>0,01) entre os tratamentos. A emulsão com óleos essenciais de orégano e alecrim (2mL kg -1 ), para leitões entre zero e o oito dias de vida, pode vir a substituir a enrofloxacina no controle dos sinais clínicos das diarreias neonatais. Palavras-Chave: enrofloxacina; Origanum vulgare; Rosmarinus officinalis labiatae. Abstract:This study evaluated the liquid emulsion of oregano and rosemary essential oils in the control of neonatal diarrhea in piglets. We monitored 1334 piglets with average weight of 1.5 kg regarding the clinical signs of neonatal diarrhea. The design was completely randomized with three treatments (T): (T1) control (saline), (T2) essential oils and (T3) enrofloxacin. T1 showed characteristic

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“…It was suggested previously that the number and function of leukocytes can vary depending on the reproductive stage and the changes in hormonal concentrations (16). Especially, 17β-oestradiol (E2) increased the proliferative response to mitogens, as well as the antibody response to antigens of porcine purified mononuclear cells (12).…”

mentioning

confidence: 99%

Elastase, Myeloperoxidase, and Alkaline Phosphatase Release and Free Radical Generation in Neutrophils Isolated from Blood of Sows at Different Stages of Oestrous Cycle

Wessely-Szponder

Bobowiec

2013

Bulletin of the Veterinary Institute in Pulawy

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The aim of the study was to assess the influence of the oestrous cycle phase on neutrophil secretory activity and to extrapolate it to susceptibility to uterine infections. The obtained results indicate that the highest enzyme release seen in the late follicular phase (elastase release was 42.18 ±3.11% of maximal release, myeloperoxidase was 45.0 ±5.12%, and alkaline phosphatase was 44.75 ±9.0%) was related to the level of 17β-oestradiol in plasma. Similarly, a free radical generation was also the most enhanced during this phase. Significantly lower values were obtained from sows during the luteal phase in regard to both enzyme release (36.62 ±3.58% for elastase, 27.87 ±8.7% for myeloperoxidase, and 22.12 ±2.4% for alkaline phosphatase), and that of free radicals (2.28 1.6 μM/10 6 cells for nitric oxide and 2.47 0.6 nM/10 6 cells for superoxide).

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Sows intramammarily inoculated with Escherichia coli at parturition: I Functional capacity of granulocytes in sows affected or non-affected by clinical mastitis

Cited by 24 publications

References 26 publications

Morphometric analysis of proinflammatory cytokines in mammary glands of sows suggests an association between clinical mastitis and local production of IL-1beta, IL-6 and TNF-alpha

Morphometric analysis of proinflammatory cytokines in mammary glands of sows suggests an association between clinical mastitis and local production of IL-1beta, IL-6 and TNF-alpha

Uso De Óleos Essenciais No Controle Dos Sinais Clínicos Das Diarreias Neonatais Em Leitões Nascidos De Fêmeas Com Diferentes Ordens De Parto

Elastase, Myeloperoxidase, and Alkaline Phosphatase Release and Free Radical Generation in Neutrophils Isolated from Blood of Sows at Different Stages of Oestrous Cycle

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