Southern pine beetle: Olfactory receptor and behavior discrimination of enantiomers of the attractant pheromone frontalin

Payne, T. L.; Richerson, J. V.; Dickens, Joseph C.; West, Janet R.; Mori, Kenji; Berisford, C. Wayne; Hedden, Roy L.; Vité, J. P.; Blum, Murray S.

doi:10.1007/bf00994788

Cited by 55 publications

(21 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The greater olfactory sensitivity of both sexes for (+)-endo-brevicomin and (-)-frontalin than for the respective antipodes ( Fig. 5; Payne et al 1982) mirrors the greater production of these enantiomers by the insects. Thus, the totality of evidence indicates that D. frontalis is utilizing only (+)-endo-brevicomin, the attractive enantiomer as shown by VitC et al (1985).…”

Section: Discussionmentioning

confidence: 88%

Evidence that (+)-endo-Brevicomin is a Male-Produced Component of the Southern Pine Beetle Aggregation Pheromone

et al. 2007

View full text Add to dashboard Cite

Previous research indicated that the aggregation pheromone of the southern pine beetle, Dendroctonus frontalis, is produced only by females, the sex that initiates attacks. We provide evidence indicating that secondarily arriving males augment mass aggregation by releasing the attractive synergist (+)-endo-brevicomin. Healthy pines artificially infested with both sexes of D. frontalis were significantly more attractive to conspecifics than trees infested solely with females. Coupled gas chromatography-electroantennographic detection (GC-EAD) analyses of volatiles isolated from male beetles revealed substantially greater olfactory sensitivity by D, frontalis to endo-brevicomin than to any other component. The threshold of detection of both sexes for (+)-endo-brevicomin was four orders of magnitude lower than for its antipode and at least one order of magnitude lower than for either enantiomer of frontalin, the major female-produced aggregation pheromone component. Pairing with a female in a gallery stimulated individual male beetles to produce hundreds of nanograms of (+)-endo-brevicomin. (4-)-endo-Brevicomin was detected in a small percentage of female D. frontalis, whereas (-)-endo-brevicomin was never detected in either sex. In field trapping bioassays, we confirmed that (+)-endo-brevicomin is a potent synergist for attractive combinations of frontalin and pine turpentine. However, (+)-endobrevicomin failed to attract D. frontalis either when presented alone or in combination with turpentine. We postulate that mass colonization of host trees by D. frontalis is mediated by distinct semiochemicals from both sexes rather than females alone. Our discovery of a key aggregation pheromone component in such an apparently well-studied species implies that the pheromone models of other bark beetles could benefit from systematic reexamination using newer technologies. Additionally, baits fortified with (+)-endo-brevicomin may enhance pest management strategies that exploit attractants for D. frontalis.

show abstract

Section: Discussionmentioning

confidence: 88%

Evidence that (+)-endo-Brevicomin is a Male-Produced Component of the Southern Pine Beetle Aggregation Pheromone

et al. 2007

View full text Add to dashboard Cite

show abstract

“…Figure 5 3 , and (3) generalist types that respond to host monoterpenes as well as pheromones to some extent (Mustaparta et al, 1980;Payne et al, 1982;Dickens et al, 1985;Dickens, 1986). The technique of differential adaptation first attenuates an electrophysiological response by exposing a receptor cell (or antenna) to a high concentration of a compound until specific acceptor sites are saturated, then these sites are exposed to a different volatile to see if any responses are elicited (Payne and Dickens, 1976;Payne, 1979;Dickens et al, 1985).…”

Section: Sensory Organsmentioning

confidence: 99%

“…(+)-or (-)-ipsdienol in I. paraconfusus (Mustaparta et al, 1980)l or both types of chiral acceptors on the same cell [e.g. (+)-and (-)-frontalin in D. frontalis (Payne et al, 1982) and D. pseudotsugae (Dickens et al,l985)I.…”

Section: Sensory Organsmentioning

confidence: 99%

Host-Tree Chemistry Affecting Colonization in Bark Beetles

Byers¹

1995

Chemical Ecology of Insects 2

150

128

View full text Add to dashboard Cite

“…)-trans-verbenol Renwick et al (1976). Enantiomeric specificity in bark beetle responses to semiochemicals is common and well documented (Seybold 1993), and, in the case of D. frontalis, differing behavioral or olfactory responses have been demonstrated with regard to enantiomers of its pheromone components frontalin (Payne et al 1982), endo-brevicomin (Vité et al 1985;Sullivan et al 2007;Sullivan and Mori 2009;Sullivan et al 2011) and verbenone (Salom et al 1992).…”

Section: Discussionmentioning

confidence: 99%

Enantiospecific responses of southern pine beetle (Dendroctonus frontalis) and its clerid predator, Thanasimus dubius, to α-pinene

et al. 2014

View full text Add to dashboard Cite

Multi-trophic interactions between pine bark beetles, their host trees, and predators are mediated in part by volatile terpenes in host tree oleoresin that can influence aggregation and/or host finding by both prey and predator species. The southern pine beetle, Dendroctonus frontalis Zimmermann, mass-attacks pine trees in response to its aggregation pheromone combined with host resin odors including a-pinene. We investigated discrimination of apinene enantiomers in olfactory responses of D. frontalis, and in behavioral responses of D. frontalis and its major clerid predator, Thanasimus dubius (Fabricius). Trapping trials in the spring and fall assessed attraction of both species to the D. frontalis aggregation pheromone components, frontalin and endo-brevicomin, either alone or in combination with a-pinene lures of differing enantiomeric compositions. a-Pinene lures enriched with the (?)-enantiomer (97.5 %) increased D. frontalis catches significantly more than (-)-enriched (93 %) lures, but did not differ from racemic lures. Sexes of D. frontalis did not differ from one another in their responses to the enantiomers, and there was no difference in lure discrimination by D. frontalis and T. dubius. Thanasimus dubius did not distinguish between different a-pinene enantiomers. Dose-response electroantennogram studies of D. frontalis indicated that their antennae had a slightly lower response threshold to (?) than (-)-a-pinene. Each enantiomer habituated the antennae more to itself than to its antipode, implying the existence of olfactory receptors with differing affinities for enantiomers. The preference of D. frontalis for (?)-apinene may affect its host selection behavior, and should be considered in population monitoring lures.

show abstract

Southern pine beetle: Olfactory receptor and behavior discrimination of enantiomers of the attractant pheromone frontalin

Cited by 55 publications

References 33 publications

Evidence that (+)-endo-Brevicomin is a Male-Produced Component of the Southern Pine Beetle Aggregation Pheromone

Evidence that (+)-endo-Brevicomin is a Male-Produced Component of the Southern Pine Beetle Aggregation Pheromone

Host-Tree Chemistry Affecting Colonization in Bark Beetles

Enantiospecific responses of southern pine beetle (Dendroctonus frontalis) and its clerid predator, Thanasimus dubius, to α-pinene

Contact Info

Product

Resources

About