Sources of variation in fluorometry of zinc protoporphyrin in blood.

Granjean, Philippe; J, Lintrup

doi:10.5271/sjweh.2542

Cited by 4 publications

(3 citation statements)

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“…However, urinary lead excretion is high, while the heme biosynthesis may not be much affected. 29 Knutti et al30 obtained good precision even at low lead levels by adding NH4NO3 to the sample in an attempt to remove chloride as NH4Cl during the ashing phase, thereby eliminating the interference with lead from NaCI during the atomization phase. Lead in acidified urine can be determined accurately under the s optimal conditions developed in the present study.…”

Section: Discussionmentioning

confidence: 99%

Optimization of Graphite Furnace Atomic Absorption Spectrophotometry for Determination of Trace Cadmium, Lead and Nickel in Urine

Lin

1991

ANAL. SCI.

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Section: Discussionmentioning

confidence: 99%

Optimization of Graphite Furnace Atomic Absorption Spectrophotometry for Determination of Trace Cadmium, Lead and Nickel in Urine

Lin

1991

ANAL. SCI.

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“…An important limitation of the hematofluorometer is that the signal detected is influenced by background fluorescence of other blood constituents, such as bilirubin [16,[34][35][36][37], causing a falsely elevated ZnPP/heme ratio [35,[37][38][39]. Washing the erythrocytes [2,36,37,39,40] can largely eliminate such background interference, but requires anticoagulated blood obtained by venipuncture rather than a drop of capillary blood from finger puncture, an extended sample preparation time, additional laboratory facilities and trained personnel.…”

Section: Introductionmentioning

confidence: 99%

Dual‐wavelength excitation for fluorescence‐based quantification of zinc protoporphyrin IX and protoporphyrin IX in whole blood

Hennig

Gruber

Vogeser

et al. 2013

Journal of Biophotonics

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Journal of BIOPHOTONICSQuantification of erythrocyte zinc protoporphyrin IX (ZnPP) and protoporphyrin IX (PPIX), individually or jointly, is useful for the diagnostic evaluation of iron deficiency, iron-restricted erythropoiesis, lead exposure, and porphyrias. A method for simultaneous quantification of ZnPP and PPIX in unwashed blood samples is described, using dual-wavelength excitation to effectively eliminate background fluorescence from other blood constituents. In blood samples from 35 subjects, the results of the dualwavelength excitation method and a reference high performance liquid chromatography (HPLC) assay were closely correlated both for ZnPP (r s ¼ 0.943, p < 0.0001; range 37-689 mmol ZnPP/mol heme, 84-1238 nmol/L) and for PPIX (r s ¼ 0.959, p < 0.0001; range 42-4212 mmol PPIX/mol heme, 93-5394 nmol/L). In addition, for ZnPP, the proposed method is compared with conventional single-wavelength excitation and with commercial front-face fluorimetry of washed erythrocytes and whole blood. We hypothesize that dual-wavelength excitation fluorimetry will provide a new approach to the suppression of background fluorescence in blood and tissue measurements of ZnPP and PPIX.

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“…22 However, the signal detected is influenced by background fluorescence of other blood constituents. 16,[23][24][25][26][27] Potential elimination of background fluorescence entails further requirements, e.g., extended sample preparation time, additional laboratory equipment, and trained personnel. The application of this device is thus restricted if cost-effective measurements are needed, laboratory infrastructure is not available, or venipuncture is not feasible, e.g., in the case of point-of-care screening for iron deficiency under field conditions.…”

Section: Diagnostic Problemmentioning

confidence: 99%

Medical laser application: translation into the clinics

Sroka¹,

Stepp²,

Hennig³

et al. 2015

J. Biomed. Opt

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Medical laser applications based on widespread research and development is a very dynamic and increasingly popular field from an ecological as well as an economic point of view. Conferences and personal communication are necessary to identify specific requests and potential unmet needs in this multi- and interdisciplinary discipline. Precise gathering of all information on innovative, new, or renewed techniques is necessary to design medical devices for introduction into clinical applications and finally to become established for routine treatment or diagnosis. Five examples of successfully addressed clinical requests are described to show the long-term endurance in developing light-based innovative clinical concepts and devices. Starting from laboratory medicine, a noninvasive approach to detect signals related to iron deficiency is shown. Based upon photosensitization, fluorescence-guided resection had been discovered, opening the door for photodynamic approaches for the treatment of brain cancer. Thermal laser application in the nasal cavity obtained clinical acceptance by the introduction of new laser wavelengths in clinical consciousness. Varicose veins can be treated by innovative endoluminal treatment methods, thus reducing side effects and saving time. Techniques and developments are presented with potential for diagnosis and treatment to improve the clinical situation for the benefit of the patient.

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Sources of variation in fluorometry of zinc protoporphyrin in blood.

Abstract: Sources of variation in fluorometry of zinc protoporphyrin in blood. by Granjean P, Lintrup J Key terms: blood; fluorometry; letter to the editor; variation; zinc protoporphyrin This article in PubMed: www.ncbi.nlm.nih.gov/pubmed/7347917

Cited by 4 publications

References 0 publications

Optimization of Graphite Furnace Atomic Absorption Spectrophotometry for Determination of Trace Cadmium, Lead and Nickel in Urine

Optimization of Graphite Furnace Atomic Absorption Spectrophotometry for Determination of Trace Cadmium, Lead and Nickel in Urine

Dual‐wavelength excitation for fluorescence‐based quantification of zinc protoporphyrin IX and protoporphyrin IX in whole blood

Medical laser application: translation into the clinics

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