Sources and dynamics of fluorescent particles in hospitals

Pereira, Marcelo Luiz; Knibbs, Luke D.; He, Congrong; Grzybowski, Piotr; Johnson, Graham R.; Huffman, J. A.; Bell, Scott C.; Wainwright, Claire; Matte, Darlan Laurício; Dominski, Fábio Hech; Andrade, Alexandro; Morawska, Lidia

doi:10.1111/ina.12380

Cited by 31 publications

(27 citation statements)

References 52 publications

(131 reference statements)

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“…Autofluorescence under this condition is associated with biochemical fluorophores, such as metabolic function riboflavin and reduced pyridine nucleotides coenzymes (eg, NAD(P)H), which are active cellular metabolism fingerprints . In prior studies, the UVAPS has been utilized to measure outdoor and indoor bioaerosols, such as bacterial and fungal spores . Studies have shown that potential abiotic fluorescent interferents, such as humiclike substances, polycyclic aromatic hydrocarbons (PAH), certain secondary organic aerosols (SOA), soot, and mineral dust, can contribute to false‐positive counts .…”

Section: Methodsmentioning

confidence: 99%

“…[27][28][29] In prior studies, the UVAPS has been utilized to measure outdoor 25,[30][31][32][33] and indoor bioaerosols, such as bacterial and fungal spores. 14,15,34 Studies have shown that potential abiotic fluorescent interferents, such as humiclike substances, polycyclic aromatic hydrocarbons (PAH), certain secondary organic aerosols (SOA), soot, and mineral dust, can contribute to false-positive counts. [35][36][37] For outdoor conditions, the interference of abiotic materials was found to be weak for particles larger than 1 μm.…”

Section: Instrumentationmentioning

confidence: 99%

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Fluorescent biological aerosol particles: Concentrations, emissions, and exposures in a northern California residence

et al. 2018

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Residences represent an important site for bioaerosol exposure. We studied bioaerosol concentrations, emissions, and exposures in a single-family residence in northern California with 2 occupants using real-time instrumentation during 2 monitoring campaigns (8 weeks during August-October 2016 and 5 weeks during January-March 2017). Time- and size-resolved fluorescent biological aerosol particles (FBAP) and total airborne particles were measured in real time in the kitchen using an ultraviolet aerodynamic particle sizer (UVAPS). Time-resolved occupancy status, household activity data, air-change rates, and spatial distribution of size-resolved particles were also determined throughout the house. Occupant activities strongly influenced indoor FBAP levels. Indoor FBAP concentrations were an order of magnitude higher when the house was occupied than when the house was vacant. Applying an integral material-balance approach, geometric mean of total FBAP emissions from human activities observed to perturb indoor levels were in the range of 10-50 million particles per event. During the summer and winter campaigns, occupants spent an average of 10 and 8.5 hours per day, respectively, awake and at home. During these hours, the geometric mean daily-averaged FBAP exposure concentration (1-10 μm diameter) was similar for each subject at 40 particles/L for summer and 29 particles/L for winter.

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Section: Methodsmentioning

confidence: 99%

Section: Instrumentationmentioning

confidence: 99%

Fluorescent biological aerosol particles: Concentrations, emissions, and exposures in a northern California residence

et al. 2018

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“…Non-fluorescent particles were a factor of 4 greater in the adults' hospital when compared to the children's one (1.20 × 10 3 particles/L vs. 0.33 × 10 3 particles/L,). It was noted that while the overall Atmosphere 2018, 9, 1 11 of 39 fluorescent concentrations were higher in the adults' hospital, the proportion of fluorescent particles was higher in the children's hospital [105].…”

Section: Indoor Campaignsmentioning

confidence: 98%

“…The UV-APS has been deployed in a number of indoor sites, such as hospitals, examination rooms, lecture theatres, and flood-affected housing [102][103][104][105][106]. Such studies have generally examined the release of particles and the calculation of emission factors.…”

Section: Indoor Campaignsmentioning

confidence: 99%

Review: The Use of Real-Time Fluorescence Instrumentation to Monitor Ambient Primary Biological Aerosol Particles (PBAP)

Fennelly

Sewell²,

Prentice

et al. 2017

Atmosphere

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Primary biological aerosol particles (PBAP) encompass many particle types that are derived from several biological kingdoms. These aerosol particles can be composed of both whole living units such as pollen, bacteria, and fungi, as well as from mechanically formed particles, such as plant debris. They constitute a significant proportion of the overall atmospheric particle load and have been linked with adverse health issues and climatic effects on the environment. Traditional methods for their analysis have focused on the direct capture of PBAP before subsequent laboratory analysis. These analysis types have generally relied on direct optical microscopy or incubation on agar plates, followed by time-consuming microbiological investigation. In an effort to address some of these deficits, real-time fluorescence monitors have come to prominence in the analysis of PBAP. These instruments offer significant advantages over traditional methods, including the measurement of concentrations, as well as the potential to simultaneously identify individual analyte particles in real-time. Due to the automated nature of these measurements, large data sets can be collected and analyzed with relative ease. This review seeks to highlight and discuss the extensive literature pertaining to the most commonly used commercially available real-time fluorescence monitors (WIBS, UV-APS and BioScout). It discusses the instruments operating principles, their limitations and advantages, and the various environments in which they have been deployed. The review provides a detailed examination of the ambient fluorescent aerosol particle concentration profiles that are obtained by these studies, along with the various strategies adopted by researchers to analyze the substantial data sets the instruments generate. Finally, a brief reflection is presented on the role that future instrumentation may provide in revolutionizing this area of atmospheric research.

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“…The Ultraviolet Aerodynamic Particle Sizer (UV-APS; TSI, Shoreview, MN, USA) was marketed widely and, though discontinued, is still used for research purposes. The UV-APS has been applied to both indoor (Bhangar et al 2016;Kanaani et al 2008;Pereira et al 2017) and outdoor PBAP analyses (Hallar et al 2011;Huffman et al 2013;P€ oschl et al 2010;Schumacher et al 2013;Valsan et al 2016;Wei et al 2016) as well as to investigate airborne microorganism viability and dynamics (Agranovski et al 2003a;Agranovski et al 2004;Pan et al 2014a;Saari et al 2015). Pulsed 355nm light from a Nd:YAG laser excites fluorescence and the integrated intensity from 420 to 575 nm is measured (Brosseau et al 2000;Hairston, Ho, and Quant 1997).…”

Section: Lif For Early Warning Of Human Pathogensmentioning

confidence: 99%

Real-time sensing of bioaerosols: Review and current perspectives

Huffman

Perring

Savage

et al. 2019

Aerosol Science and Technology

161

129

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Detection of bioaerosols, or primary biological aerosol particles (PBAPs), has become increasingly important for a wide variety of research communities and scientific questions. In particular, real-time (RT) techniques for autonomous, online detection and characterization of PBAP properties in both outdoor and indoor environments are becoming more commonplace and have opened avenues of research. With advances in technology, however, come challenges to standardize practices so that results are both reliable and comparable across technologies and users. Here, we present a critical review of major RT instrument classes that have been applied to PBAP research, especially with respect to environmental science, allergy monitoring, agriculture, public health, and national security. Eight major classes of RT techniques are covered, including the following: (i) fluorescence spectroscopy, (ii) elastic scattering, microscopy, and holography, (iii) Raman spectroscopy, (iv) mass spectrometry, (v) breakdown spectroscopy, (vi) remote sensing, (vii) microfluidic techniques, and (viii) paired aqueous techniques. For each class of technology we present technical limitations, misconceptions, and pitfalls, and also summarize best practices for operation, analysis, and reporting. The final section of the article presents pressing scientific questions and grand challenges for RT sensing of PBAP as well as recommendations for future work to encourage high-quality results and increased cross-community collaboration.

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Sources and dynamics of fluorescent particles in hospitals

Cited by 31 publications

References 52 publications

Fluorescent biological aerosol particles: Concentrations, emissions, and exposures in a northern California residence

Fluorescent biological aerosol particles: Concentrations, emissions, and exposures in a northern California residence

Review: The Use of Real-Time Fluorescence Instrumentation to Monitor Ambient Primary Biological Aerosol Particles (PBAP)

Real-time sensing of bioaerosols: Review and current perspectives

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