Soroprevalência do toxoplasma gondii, em suínos, bovinos, ovinos e eqüinos, e sua correlação com humanos, felinos e caninos, oriundos de propriedades rurais do norte do Paraná-Brasil

Garcia, João Luís; Navarro, Italmar Teodorico; Ogawa, Liza; Oliveira, Renata Cristina Gobbi de

doi:10.1590/s0103-84781999000100017

Cited by 102 publications

(104 citation statements)

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“…The used PCR amplified a 529bp fragment that is repeated 200-300 times in T. gondii genome, and it was described as being more sensitive than B1 PCR (Homan et al, 2000). However, more DNA targets should be tested to ensure progress in the molecular diagnosis of toxoplasmosis (Chabbert et al, 2004).…”

Section: Resultsmentioning

confidence: 99%

“…DNA amplification of T. gondii was performed using the method described by Homan et al (2000). Primers and TOX5 (5'-CGCTGCAGACACAGTGCATCTGGATT-3') were used, and these flanked a 529bp fragment of T. gondii DNA.…”

Section: Methodsmentioning

confidence: 99%

“…Epidemiological studies in Brazil have showed that the seroprevalence of T. gondii in pigs varies from 9.6% to 54.1% (Vidotto et al, 1990;Garcia et al, 1999;Suárez-Aranda et al, 2000). This wide variation could be related to regional/geographical factors and the difference in production systems (Garcia et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

“…This wide variation could be related to regional/geographical factors and the difference in production systems (Garcia et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

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Detection of Toxoplasma gondii by PCR and mouse bioassay in commercial cuts of pork from experimentally infected pigs

Tsutsui

Freire

Garcia

et al. 2007

Arq. Bras. Med. Vet. Zootec.

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The distribution of T. gondii in commercial cuts of pork (ham, tenderloin, spareribs and arm picnic) by PCR and bioassay from experimentally infected pigs, was evaluated. Eighteen mixed breed pigs were divided into two groups (G). The G1 animals (n=10) were infected with 4 x10 4 oocysts of the T. gondii VEG strain and the G2 animals (n=8) were used as control. Pigs of both groups were slaughtered at 59 th day after infection, and meat samples were collected for bioassay and PCR. All animals from G1 were positive by at least one or both tests, and all control animals were negative. T. gondii was identified in pork by mouse bioassay and PCR in 27/40 (67.5%) and in 9/40 (22.5%) of the evaluated samples, respectively. There were no statistical differences in the distribution of tissue cysts from commercial cuts of pork by bioassay (P>0.05). However, statistical differences were observed when mouse bioassay and PCR were compared (P<0.01). Keywords: pork meat, mouse bioassay, PCR, Toxoplasma gondii RESUMO Avaliou-se a presença de T. gondii em cortes comerciais de carne suína (pernil, lombo, costela e paleta), por meio do bioensaio e PCR, em animais experimentalmente inoculados. Dois grupos (G) foram formados. Os animais do G1 (n=10) foram inoculados com 4 x10 4 oocistos da cepa VEG e os do G2 (n=8) permaneceram como grupo-controle, não inoculado. Todos os animais foram abatidos no dia

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…This wide variation could be related to regional/geographical factors and the difference in production systems (Garcia et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Detection of Toxoplasma gondii by PCR and mouse bioassay in commercial cuts of pork from experimentally infected pigs

Tsutsui

Freire

Garcia

et al. 2007

Arq. Bras. Med. Vet. Zootec.

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“…These animals may be involved in the mechanical transmission of T. gondii to humans as reported by others (Frenkel & Parker 1996, Lindsay et al 1997, mentioned above in the underlined sentence. The most important fact, however, is that dogs and man are exposed to the same sources of T. gondii (Dubey & Beattie 1988, Cabral et al 1998, Garcia et al 1999, Cañón-Franco et al 2004, Jittapalapong et al 2007). Thus, this study aimed to determine the frequency of antibodies to Toxoplasma gondii in dogs from Ubatuba, a coastal municipality in São Paulo State, Brazil, characterized by low hygienic-sanitary conditions associated with the tropical conditions of the Brazilian coast and the risk factors that favor the infection in animals.…”

Section: Introductionmentioning

confidence: 99%

Risk factors and presence of antibodies to Toxoplasma gondii in dogs from the coast of São Paulo State, Brazil

et al. 2010

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RESUMO.-[Fatores de risco e presença de anticorpos para Toxoplasma gondii em cães do litoral do Estado de São Paulo.] A toxoplasmose é causada por um protozoário parasita intracelular obrigatório, Toxoplasma gondii, e acomete vertebrados homeotérmicos incluindo animais de companhia e o homem. O cão apresenta importância epidemiológica por atuar como sentinela da infecção para o homem. O presente estudo objetivou determinar a ocorrência de anticorpos para T. gondii em 205 amostras de soro de cães do município de Ubatuba, SP, Brasil, pela reação de imunofluorescência indireta (RIFI), assim como os fatores de risco relacionados à infecção nos animais, como raça, idade, sexo, acesso a rua, ingestão de comida caseira, acesso a água não tratada e presença de roedores. 52/205 (25,4%) Toxoplasmosis is caused by the obligate intracellular protozoan parasite Toxoplasma gondii and affects warm-blooded vertebrates, including pets and man. Dogs are epidemiologically important since they act as sentinels for the infection in humans. The present study aimed to determine the presence of antibodies to T. gondii in 205 serum samples from dogs in Ubatuba, Sao Paulo state, Brazil, through indirect immunofluorescence reaction (IFAT), as well as the risk factors related to toxoplasmosis in the animals such as breed, age, sex, access to outdoors, homemade food ingestion, access to untreated water, and contact with rodents. Toxoplasmosis-positive samples accounted for 52/205 (25.4%), with titers ranging from 16 to 256. The serological results presented significant association (P<0.05) with homemade food ingestion (45/118; 38.1%; CI95% 29.9%-47.2%) (OR=7.0; CI95% 3.0-16.6), and with access to outdoors where those that do not have access to the street were prevalent (37/121; 30.6%; CI95% 23.1%-39.3%) (OR=0.5; CI95% 0.2-1.0). These results show that toxoplasmosis in this region is related to problems of sanitary education, mainly concerning the appropriate cooking of foods, since most positive animals did not show significant association with the presence of rodents or untreated water consumption but showed, instead association with ingestion of homemade food. Thus, toxoplasmosis is a public health problem in the studied region, and sanitary measures are needed to control the infection due to the strict relationship between man and dog and the presented risk factors. e acesso a rua, em que aqueles que não tinham acesso a rua foram prevalentes (37/121; 30,6%; IC95% 23,1%-39,3%) (OR=0,5; CI95% 0,2-1,0). Estes resultados demonstram que a toxoplasmose na região está relacionada com problema de educação sanitária, principalmente quanto ao adequado cozimento dos alimentos, visto que a maioria dos animais positivos não apresentou associação significativa com presença de roedores ou consumo de água não tratada, porém os mesmos permaneciam em casa aos quais fora oferecida comida caseira. Portanto, a toxoplasmose consiste em um problema de saúde pública na região estudada, sendo necessárias medidas sanitári-as para o controle da infecção, visto a...

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Antibodies to Neospora caninum and Toxoplasma gondii in domestic cats from Brazil

et al. 2006

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The occurrence of antibodies to Neospora caninum and Toxoplasma gondii was determined in 400 domestic cats (Siamese, Persian, and undetermined breeds) from the Municipality of Araçatuba, Sao Paulo, Brazil, through the indirect fluorescence antibody test (IFAT). Of the 400 cats, 100 were seropositive to T. gondii (25%, titer > or =64) and 98 to N. caninum (24.5%, titer > or =16). The rate of seropositive cats for T. gondii was correlated with age (chi(2)=35.7; p<0.001), with a higher number of infected animals at older ages. Of the 219 cats younger than 1-year-old, 13.2% were seropositive for T. gondii, while 39.2% were positive in the 181 older animals. The presence of N. caninum was also correlated with age (/(2)=8.8; p<0.01), with 18.7% (41/219) and 31.5% (57/181) of positive animals at ages below and above 12-month, respectively. An association between the occurrences of both protozoa in the felines was also observed (chi(2)=19.6; p<0.001).

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Soroprevalência do toxoplasma gondii, em suínos, bovinos, ovinos e eqüinos, e sua correlação com humanos, felinos e caninos, oriundos de propriedades rurais do norte do Paraná-Brasil

Cited by 102 publications

References 10 publications

Detection of Toxoplasma gondii by PCR and mouse bioassay in commercial cuts of pork from experimentally infected pigs

Detection of Toxoplasma gondii by PCR and mouse bioassay in commercial cuts of pork from experimentally infected pigs

Risk factors and presence of antibodies to Toxoplasma gondii in dogs from the coast of São Paulo State, Brazil

Antibodies to Neospora caninum and Toxoplasma gondii in domestic cats from Brazil

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