Sophora flavescens Aiton Decreases MPP+-Induced Mitochondrial Dysfunction in SH-SY5Y Cells

Kim, Hee-Young; Jeon, Hyongjun; Kim, Hyungwoo; Koo, Sungtae; Kim, Seung-Tae

doi:10.3389/fnagi.2018.00119

Cited by 25 publications

(20 citation statements)

References 51 publications

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“…Sophora species, especially SF and SJ, have been used to prevent aging-associated neurodegenerative disorders such as PD or ischemia/reperfusion in traditional Chinese medicine. Kim et al indicated that SF extract protected against MPP + -induced neurotoxicology in SH-SY5Y neuroblastoma cells [ 17 ]. ST had similar therapeutic applications to these above Sophora species, but there is no related literature.…”

Section: Discussionmentioning

confidence: 99%

“…Accumulating reports have shown that the above Sophora medicinal plants have antioxidative, anti-inflammatory, and neuroprotective effects [ 15 , 16 ]. SF extract protected against MPP + -induced and glutamate-induced neurotoxicology in SH-SY5Y neuroblastoma cells and hippocampal cells [ 17 , 18 ]. SF or SJ extracts protected neuronal damage against focal cerebral ischemia in rats [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

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Sophora Tomentosa Extract Prevents MPTP-Induced Parkinsonism in C57BL/6 Mice Via the Inhibition of GSK-3β Phosphorylation and Oxidative Stress

et al. 2019

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Sophora species are used as dietary medicines in aging-associated symptoms. Sophora tomentosa L. (ST) is a native medicinal plant in Southeast Asia; however, there is no pharmacological literature about ST extract. The present study evaluates the antioxidant phytoconstituent contents and radical scavenging capacities of ST extract. The further investigation was to clarify the neuroprotective mechanism of ST extract against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinsonism by assaying the activities of the dopaminergic system and antioxidant defenses, glycogen synthase kinase 3β (GSK3-β) phosphorylation, and α-synuclein levels in C57BL/6 mice. The results show that ST extract alleviated the motor deficits in MPTP-induced Parkinsonism with four behavioral tests, including a rearing locomotor, catalepsy test, balance beam walking test, and pole test. ST extract reversed the number of tyrosine hydroxylase (TH)-positive neurons in substantia nigra (SN) that had decreased by MPTP. ST extract also restored the decreased levels of dopamine and the expression of tyrosine hydroxylase (TH) in the striatum. Furthermore, ST extract restored the levels of glutathione (GSH) and the activities of antioxidant enzymes, and decreased the elevated levels of malondialdehyde (MDA) in mouse striatum. ST extract also decreased α-synuclein overexpression and GSK-3β phosphorylation in mouse striatum. In vitro, ST extract exerted higher 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging capacities through its higher phenolic contents, especially protocatechuic acid and epicatechin. These results suggest that ST extract has the potential to counteract MPTP-induced motor deficit. The neuroprotective mechanism of ST extract against MPTP-induced Parkinsonism might be related to decreasing GSK-3β phosphorylation and restoring the activities of striatal antioxidant defenses to restore the nigrostriatal dopaminergic function and decrease α-synuclein accumulation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sophora Tomentosa Extract Prevents MPTP-Induced Parkinsonism in C57BL/6 Mice Via the Inhibition of GSK-3β Phosphorylation and Oxidative Stress

et al. 2019

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“…As a result, recent studies are directed toward outlining the action mechanism of plant extracts and phytochemicals in the prevention and treatment of neurodegenerative diseases. In particular, Lycium chinense Miller , Sophora flavescens Aiton , and Panax ginseng demonstrated neuroprotective effects in rotenone or 1‐methyl‐4‐phenylpyridinium ion‐induced neurotoxicity (Im et al, ; Kim, Jeon, Kim, Koo, & Kim, ; Kim, Lee, et al, ). Betaine present in L. chinense M and sesquiterpenoid from Tussilago farfara also showed similar effects in rotenone and 6‐hydroxydopamine‐induced toxicity in PC‐12 cells, respectively (Im et al, ; Lee, Song, Huh, Oh, & Kim, ).…”

Section: Discussionmentioning

confidence: 99%

Chlorogenic acid‐rich Solanum melongena extract has protective potential against rotenone‐induced neurotoxicity in PC‐12 cells

Youn¹,

Jeon²,

Jin³

et al. 2019

J Food Biochem

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Neurodegenerative diseases are major threats to human health. Here, through fluorescence, colorimetric, immunoblotting, spectroscopy, and laser scanning confocal microscopic techniques, we investigated the neuroprotective properties of chlorogenic acid‐rich Solanum melongena extracts (SM extract) in rotenone‐induced PC‐12 cell death. The results showed that rotenone caused apoptosis to PC‐12 cells by elevating Bax/Bcl‐2 ratio and increasing caspase‐3 activity. Rotenone also increased ROS in cells while suppressing SOD and catalase activities. This resulted in the depletion of ATP in cells by blocking mitochondria complex I activity. Pretreatment of the cells with SM extract at concentrations of 100, 250, and 500 μg/ml before incubation for 24 hr with rotenone significantly prevented apoptosis, decreased ROS, and increased ATP production in the cells. SM extract upregulated SOD and catalase activities in the cells. These results unveil evidence that SM extract content neuroprotective properties that can be exploited to prevent and treat neurodegenerative diseases. Practical applications Solanum melongena eggplant is a popular ingredient in many traditional recipes and is well known in Asia for its medicinal benefits. Despite numerous scientific reports of the potential health benefits of this plant, reports on its effects in neurodegenerative diseases is still lacking. This pilot study demonstrates that S. melongena eggplant can protect against neurotoxicity in neurodegenerative diseases. The results of this research serves as a base for further research on eggplant that will result in its usage on a larger scale as functional food materials.

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“…To contribute PD cell culture model in vitro , 1.5 ml of 1 × 10 5 cells/ml of SH-SY5Y cells were cultured in 12-well plates for approximately 24 h and were then respectively treated with 0.1% dimethyl sulfoxide (Sigma–Adrich, St. Louis, MO, USA) containing 1 μmol/L (μM) rotenone (Sigma–Adrich, St. Louis, MO, USA) for 24 h, 1 mmol/L (mM) 1-methyl-4-phenylpyridinium (MPP + ; Sigma–Adrich, St. Louis, MO, USA) for 24 h or 100 μmol/L (μM) 6-hydroxydopamine (6-OHDA; Sigma–Adrich, St. Louis, MO, USA) for 24 h (Feng et al, 2015 ; Zhang et al, 2017 ; Kim et al, 2018 ) After being digested and washed with phosphate buffer saline, SH-SY5Y cells were subjected to further treatment and analysis. Each experiment was repeated at least three times.…”

Section: Methodsmentioning

confidence: 99%

Immune Profiling of Parkinson’s Disease Revealed Its Association With a Subset of Infiltrating Cells and Signature Genes

Zhang

Shao

et al. 2021

Front. Aging Neurosci.

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Parkinson’s disease (PD) is an age-related and second most common neurodegenerative disorder. In recent years, increasing evidence revealed that peripheral immune cells might be able to infiltrate into brain tissues, which could arouse neuroinflammation and aggravate neurodegeneration. This study aimed to illuminate the landscape of peripheral immune cells and signature genes associated with immune infiltration in PD. Several transcriptomic datasets of substantia nigra (SN) from the Gene Expression Omnibus (GEO) database were separately collected as training cohort, testing cohort, and external validation cohort. The immunoscore of each sample calculated by single-sample gene set enrichment analysis was used to reflect the peripheral immune cell infiltration and to identify the differential immune cell types between PD and healthy participants. According to receiver operating characteristic (ROC) curve analysis, the immunoscore achieved an overall accuracy of the area under the curve (AUC) = 0.883 in the testing cohort, respectively. The immunoscore displayed good performance in the external validation cohort with an AUC of 0.745. The correlation analysis and logistic regression analysis were used to analyze the correlation between immune cells and PD, and mast cell was identified most associated with the occurrence of PD. Additionally, increased mast cells were also observed in our in vivo PD model. Weighted gene co-expression network analysis (WGCNA) was used to selected module genes related to a mast cell. The least absolute shrinkage and selection operator (LASSO) analysis and random-forest analysis were used to analyze module genes, and two hub genes RBM3 and AGTR1 were identified as associated with mast cells in the training cohort. The expression levels of RBM3 and AGTR1 in these cohorts and PD models revealed that these hub genes were significantly downregulated in PD. Moreover, the expression trend of the aforementioned two genes differed in mast cells and dopaminergic (DA) neurons. In conclusion, this study not only exhibited a landscape of immune infiltrating patterns in PD but also identified mast cells and two hub genes associated with the occurrence of PD, which provided potential therapeutic targets for PD patients (PDs).

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Sophora flavescens Aiton Decreases MPP+-Induced Mitochondrial Dysfunction in SH-SY5Y Cells

Cited by 25 publications

References 51 publications

Sophora Tomentosa Extract Prevents MPTP-Induced Parkinsonism in C57BL/6 Mice Via the Inhibition of GSK-3β Phosphorylation and Oxidative Stress

Sophora Tomentosa Extract Prevents MPTP-Induced Parkinsonism in C57BL/6 Mice Via the Inhibition of GSK-3β Phosphorylation and Oxidative Stress

Chlorogenic acid‐rich Solanum melongena extract has protective potential against rotenone‐induced neurotoxicity in PC‐12 cells

Immune Profiling of Parkinson’s Disease Revealed Its Association With a Subset of Infiltrating Cells and Signature Genes

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