Sonodynamic effects of hematoporphyrin monomethyl ether on<i>Staphylococcus aureus in vitro</i>

Zhuang, Deshu; Hou, Chunyan; Bi, Liangjia; Han, Jialong; Yu, Hao; Cao, Wenwu; Zhou, Qi

doi:10.1111/1574-6968.12628

Cited by 36 publications

(49 citation statements)

References 32 publications

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“…But the sensitizers have noncytotoxicity in a certain concentration range and do not damage the surrounding normal tissues [24, 25]. HMME possess a stable wavelength and a short incubation time, compared with hematoporphyrin derivative (HPD) [13]. Previous studies have indicated that the HMME concentration of 40 μ g/mL activated by laser reduced the growth of THP-1 cells [26].…”

Section: Discussionmentioning

confidence: 99%

“…Using this technique, sonosensitizer is activated by irradiation with low-intensity ultrasound of a suitable frequency, generating reactive oxygen species (ROS), such as free radicals and singlet oxygen, to kill target cells [11]. Based on the characteristic of ultrasound, SDT has many advantages over conventional periodontal treatment, such as no drug resistance, noninvasive, easy repeatability, and strong penetrating power [13]. Recent experimental studies showed that SDT can effectively kill Staphylococcus aureus [13], methicillin-resistant Staphylococcus aureus [14], and inflammatory cells, such as U937 and THP-1 [15, 16].…”

Section: Introductionmentioning

confidence: 99%

“…Based on the characteristic of ultrasound, SDT has many advantages over conventional periodontal treatment, such as no drug resistance, noninvasive, easy repeatability, and strong penetrating power [13]. Recent experimental studies showed that SDT can effectively kill Staphylococcus aureus [13], methicillin-resistant Staphylococcus aureus [14], and inflammatory cells, such as U937 and THP-1 [15, 16]. However, the effect of SDT using 3 W/cm 2 low-intensity ultrasound on experimental periodontal disease in rats has not been clarified.…”

Section: Introductionmentioning

confidence: 99%

“…It consists of two positional isomers of 3-(1-methyloxyethyl)-8-(1-hydroxyethyl) deuteroporphyrin IX and 8-(1-methyloxyethyl)-3-(1-hydroxyethyl) deuteroporphyrin IX [13]. Some researches indicated that HMME can be a sonosensitizer in SDT to inhibit cancer cells, inflammatory cells, and bacteria [13, 15]. After being activated by ultrasound, HMME generated ROS to induce cell apoptosis through the mitochondrial apoptotic pathway [17].…”

Section: Introductionmentioning

confidence: 99%

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Low-Intensity Ultrasound Combined with Hematoporphyrin Monomethyl Ether in the Treatment of Experimental Periodontitis in Rats

Zhuang

et al. 2016

BioMed Research International

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Objectives. This study aims to evaluate the efficacy of hematoporphyrin monomethyl ether- (HMME-) mediated sonodynamic therapy (SDT) on experimental periodontal disease in rats. Methods. Periodontal disease was induced by submerging ligatures at the first maxillary molar subgingival region in forty-eight male SD rats. After 30 days, the ligatures were removed. The rats were randomly allocated into four groups; the experimental SDT group was treated through hypodermic injection of 40 μg/mL HMME and 3 W/cm2 low-intensity ultrasound irradiation (1 MHz, 600 s). Those in control groups received 40 μg/mL HMME alone (control 1 group) or 3 W/cm2 ultrasound irradiation alone (control 2 group) or were subjected to neither HMME nor ultrasound (control 3 group). After 10 days of treatment, all rats were euthanized, the maxilla was obtained for histological examination, and the alveolar bone level was evaluated by histometric analysis. Results. The control groups showed more bone loss (P < 0.05) after 10 days of treatment than the SDT group. There is no significant difference among the control groups (P > 0.05). Conclusions. HMME mediated SDT was an effective therapy of experimental periodontal tissue in rats.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Low-Intensity Ultrasound Combined with Hematoporphyrin Monomethyl Ether in the Treatment of Experimental Periodontitis in Rats

Zhuang

et al. 2016

BioMed Research International

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“…Zhou and co-workers studied the effect of hematoporphyrin monomethyl ester (HMME) in the SACT of S. aureus. 54 The authors investigated the effect of the sensitiser dose and the ultrasound dose on the cell inactivation efficacy, and they found that under the most effective SACT parameters, which involved the incubation with 50 µg/L HMME followed by US irradiation (1 MHz, 6 W/cm 2 , 30 min), the reduction in microbial load was ca. 2 log.…”

Section: Sact Mediated By Photosensitisersmentioning

confidence: 99%

Sonodynamic antimicrobial chemotherapy: First steps towards a sound approach for microbe inactivation

Serpe

Giuntini

2015

Journal of Photochemistry and Photobiology B: Biology

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Sonodynamic therapy (SDT) relies on the ability of ultrasound to activate sonosensitisers and trigger the generation of reactive oxygen species (ROS) to achieve cell death. SDT was explored as an anticancer approach until 6 years ago, when its potential application as an antimicrobial strategy was pointed out and the term "sonoantimicrobial chemotherapy" (SACT) was coined. The excellent penetration of ultrasound in liquid media make SACT particularly promising approach for the noninvasive treatment of deep-seated infections, and for the reduction of bacterial load in turbid water. In this review we provide an account of the brief history of SACT, from its molecular bases to the current state of the art and perspective applications. Graphical abstract

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Different Aspects of Using Ultrasound to Combat Microorganisms

Nakonechny

Nisnevitch

2021

Adv Funct Materials

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Growing resistance of microorganisms to antibiotics due to their widespread use has led to multiple bacterial infections posing a serious threat to health and human life. Low-frequency ultrasound is one of physical methods for inactivation of pathogenic microbial cells. Application of ultrasound is safe, demonstrates good tissue penetration without significant attenuation of energy, and does not induce microbial resistance. Bactericidal effect of ultrasound is based on acoustic cavitation-the growth and collapse of microbubbles in a liquid medium, resulting in shock waves, shear forces, and microjets which cause irreversible damage and inactivation of microorganisms. The present review combines and analyzes literature data on in vitro and in vivo studies and summarizes works demonstrating the ability of ultrasound, alone or in combination with other methods, to combat pathogenic microorganisms. The results of various studies presented in this review show that low-frequency ultrasound has a noticeable antimicrobial effect on planktonic cells of microorganisms and biofilms. Ultrasound synergistically enhances the effectiveness of other antibacterial agents and activates molecules called sonosensitizers, resulting in the formation of compounds toxic to microbial cells. Ultrasound can also promote local release of antimicrobial drugs from liposomes, as well as from medical implants.

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Sonodynamic effects of hematoporphyrin monomethyl ether onStaphylococcus aureus in vitro

Cited by 36 publications

References 32 publications

Low-Intensity Ultrasound Combined with Hematoporphyrin Monomethyl Ether in the Treatment of Experimental Periodontitis in Rats

Low-Intensity Ultrasound Combined with Hematoporphyrin Monomethyl Ether in the Treatment of Experimental Periodontitis in Rats

Sonodynamic antimicrobial chemotherapy: First steps towards a sound approach for microbe inactivation

Different Aspects of Using Ultrasound to Combat Microorganisms

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