Sonication of catheter tips for improved detection of microorganisms on external ventricular drains and ventriculo-peritoneal shunts

Jost, Gregory F.; Wasner, Morten; Taub, Ethan; Walti, Laura N; Mariani, Luigi; Trampuž, Andrej

doi:10.1016/j.jocn.2013.05.025

Cited by 33 publications

(18 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In regard to ATP levels, post-cleaning were comparable with pre-contamination results. After contamination, ATP levels reached a median of 8434 (range: 1385-727668) RLUs, whereas after cleaning levels decreased to 35 (range: 11-220) RLUs, comparable with the pre-contamination levels of 28 (21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40) RLUs. After cleaning, only one catheter displayed more than 100 RLUs (220 RLUs,…”

Section: Adenosine Triphosphate Testsupporting

confidence: 54%

“…After the irrigation with 60 mL of sterile water, the 5 fragments of each catheter were cut in small pieces, which were sonicated at 40 kHz for 4 minutes, and then vortexed for 1 minute, with 20 mL of sterile distilled water, in sterile tubes, as adapted from previous publications 26,27 . The resulting solution was then transferred to another fresh tube and centrifuged at 4000 rpm for 15 minutes.…”

Section: Viable Bacteria Recovery and Biofilm Analysismentioning

confidence: 99%

See 1 more Smart Citation

Effectiveness of current disinfection procedures against biofilm on contaminated GI endoscopes

Neves

Silva

Ventura

et al. 2016

Gastrointestinal Endoscopy

View full text Add to dashboard Cite

Section: Adenosine Triphosphate Testsupporting

confidence: 54%

Section: Viable Bacteria Recovery and Biofilm Analysismentioning

confidence: 99%

Effectiveness of current disinfection procedures against biofilm on contaminated GI endoscopes

Neves

Silva

Ventura

et al. 2016

Gastrointestinal Endoscopy

View full text Add to dashboard Cite

“…However, isolated positive cultures from broth media (i.e., thioglycolate broth or blood culture) must be interpreted in the context of the overall clinical likelihood of infection, as they often represent bacterial colonization or contamination (27,33,43). Sonication cultures of explanted neurosurgical hardware (e.g., EVD tip) may also increase the yield of cultures and diagnosis of infections when clinical suspicion is high, similar to sonication cultures of orthopedic hardware (47,48). Conventional blood cultures are generally not helpful in diagnosing most CNS device infections but can be helpful and should be performed in patients with suspicion for ventriculoatrial shunt infection (15,39).…”

Section: Microbiologic Testingmentioning

confidence: 99%

Diagnostic Approach to Health Care- and Device-Associated Central Nervous System Infections

et al. 2018

View full text Add to dashboard Cite

Health care- and device-associated central nervous system (CNS) infections have a distinct epidemiology, pathophysiology, and microbiology that require a unique diagnostic approach. Most clinical signs, symptoms, and tests used to diagnose community-acquired CNS infections are insensitive and nonspecific in neurosurgical patients due to postsurgical changes, invasive devices, prior antimicrobial exposure, and underlying CNS disease. The lack of a standardized definition of infection or diagnostic pathway has added to this challenge. In this review, we summarize the epidemiology, microbiology, and clinical presentation of these infections, discuss the issues with existing microbiologic tests, and give an overview of the current diagnostic approach.

show abstract

“…A recent large study on the incidence of infections following the introduction of an external ventriculostomy catheter was conducted by Bota et al 7 In diagnosing IVC-related infections, the problem is the isolation of microorganisms from the surface of the catheter. Jost et al (22) showed that the sonication of the removed catheter fragments increased the number of positive samples (64%, 9/14) compared to the CSF aspirate (14%, 2/14). In the own study, sonication was also used to detach biofilm cells from the surface of the catheters.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Formation of Single- and Double-Species Biofilms on Intraventricular Catheters by Strains of Staphylococcus aureus, Listeria monocytogenes and Escherichia coli with K1 Antigen

Skowron

Grudlewska

Kaczmarek

et al. 2018

Jundishapur J Microbiol

View full text Add to dashboard Cite

Background: Intraventricular catheters (IVCs) are an important diagnostic and therapeutic tool. They are used in patients after traumatic brain injury, intracranial haemorrhage, ventricular, or hydrocephalus. However, the use of IVCs may lead to the development of bacterial brain and cerebrovascular infections associated with the presence of microorganisms in cerebrospinal fluid (ventriculitis). What is especially dangerous for the patients health is the formation of biofilms by microorganisms on the catheters. Objectives: The aim of the study was to evaluate the intensity of single-and double-species biofilms formation on IVCs by Staphylococcus aureus (SAU), Escherichia coli (ECO) with K1 antigen, and Listeria monocytogenes (LMO). Methods: The research material was 15 strains of S. aureus, L. monocytogenes, and E. coli with K1 antigen, each. Evaluation of biofilm formation was performed on sterile fragments of IVCs with 1 cm length. The intensity of biofilm formation by the tested strains was determined with the quantitative method using sonication for removal of bacteria from IVCs surface. Biofilm formation ability was verified individually for each of the tested bacteria strain and for SAU + LMO, SAU + ECO, LMO + ECO combinations. Randomly selected fragments of biofilm-coated catheters were subjected to scanning electron microscopy (SEM). Results: The number of bacteria isolated from single-species biofilms on the IVCs surface ranged from 3.79 × 10 7 do 8.95 × 10 7 CFU × cm-2 , depending on the species of microorganism. The strongest biofilm formed ECO with K1 antigen strains and the weakest-LMO strains. The scomposition of multi-species biofilms significantly influences the intensity of biofilm formation by strains from the examined species. In the SAU + LMO combination, strains from both species formed biofilm more intensively than in single-species variant. In turn, ECO has shown a significant inhibitory effect on biofilm formation by other tested species. Conclusions: It was stated that bacteria form biofilm on IVCs and it is a significant medical problem. The species composition of multi-species biofilms significantly influences the number of individual bacteria recovered from this biofilm.

show abstract

Sonication of catheter tips for improved detection of microorganisms on external ventricular drains and ventriculo-peritoneal shunts

Cited by 33 publications

References 28 publications

Effectiveness of current disinfection procedures against biofilm on contaminated GI endoscopes

Effectiveness of current disinfection procedures against biofilm on contaminated GI endoscopes

Diagnostic Approach to Health Care- and Device-Associated Central Nervous System Infections

Evaluation of the Formation of Single- and Double-Species Biofilms on Intraventricular Catheters by Strains of Staphylococcus aureus, Listeria monocytogenes and Escherichia coli with K1 Antigen

Contact Info

Product

Resources

About