Some Variables Affecting the Recovery of Anaerobic Bacteria: a Quantitative Study

Watt, B.; Hoare, Margaret V.; Collee, J. G.

doi:10.1099/00221287-77-2-447

Cited by 13 publications

(7 citation statements)

References 6 publications

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“…The anaerobic re-incubation of primary plates to 5 days yielded a larger number of isolates. Multiple catalyst sachets (Watt, Hoare and Collee, 1973) or an improved catalyst container (Baldwin, 1975) should be used, and the catalyst should be changed and reactivated at weekly intervals.…”

Section: Discussionmentioning

confidence: 99%

The Anaerobic Culture of Clinical Specimens: A 14-Month Study

Wren

Baldwin

Eldon

et al. 1977

Journal of Medical Microbiology

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THE isolation rate of anaerobic bacteria from clinical specimens increased manyfold in our laboratory when the techniques for anaerobic bacteriology were revised. We present here an account of our findings with these methods applied as a routine to clinical specimens, and make recommendations concerning the techniques of incubation, media and the use of certain identification tests. The isolation of anaerobic species from different anatomical sites is described, and special attention is given to the yield of organisms from empyema fluid, the peritoneal cavity, surface lesions and blood. MATERIALS AND METHODSWith the general exception of urines, faeces, respiratory specimens and cerebrospinal fluid (CSF), specimens of exudates received between 1 Dec. 1974 and 31 Jan. 1976 were cultured for anaerobic bacteria as described below. Transport of specimens. Samples of pus were transported to the laboratory in sterileMcCartney bottles. Swabs of purulent material were sometimes transported deep in a tube of Cary and Blair transport medium (Cary and Blair, 1964), but many swabs were received in the ordinary dry state.Macroscopic examination. Specimens were examined for colour, odour, consistency and the presence of sulphur granules. Liquid pus samples were examined for brick-red fluorescence under an ultraviolet light (Blak-Ray UVL 21, Ultraviolet Products Inc., San Gabriel, California) at a distance of 6-9 in. (15-23 cm) for presumptive evidence of the presence of Bacteriodes melaninogenicus.Microscopic examination. Samples of pus were examined by gram stain to ascertain the number and proportion of morphological forms present.Culture of clinical material. Our complete range of media used for culture in this study were as follows :1. Blood agar: Columbia Agar Base (London Analytical and Bacteriological Media) with 5 % defibrinated horse-blood; this was obtained as poured plates from London Analytical and Bacteriological Media Ltd, Salford, Lancs. (LAB M). 2. Supplemented brucella agar: Brucella Agar (Difco Laboratories Ltd, West Molesey, Surrey) with 5 % defibrinated horse blood and menadione 0.5 pg per ml (Sigma Chemical Co. Ltd, Kingston-upon-Thames, Surrey). 3. Supplemented selective brucella agar: supplemented brucella agar rendered selective by adding kanamycin 75 pg per ml (Kantrex solution, Bristol Laboratories, Langley, Bucks).

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Section: Discussionmentioning

confidence: 99%

The Anaerobic Culture of Clinical Specimens: A 14-Month Study

Wren

Baldwin

Eldon

et al. 1977

Journal of Medical Microbiology

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“…Improvements in anaerobic techniques (Collee, Rutter & Watt, 1971;Holdeman & Moore, 1973;Watt, 1973;Watt, Hoare & Collee, 1973;Watt, Collee & Brown, 1974) have provided laboratories with reliable methods for the isolation of bacteroides organisms, but precise identification is rarely attempted in the routine laboratory. The classification of gram-negative anaerobic bacilli has been confused, and in most laboratories methods have not been available for accurate identification.…”

Section: Introductionmentioning

confidence: 99%

The identification of gram-negative anaerobic bacilli isolated from clinical infections

Duerden

1980

J. Hyg.

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SUMMARYGram-negative anaerobic bacilli isolated from specimens submitted to the routine diagnostic bacteriology laboratory and regarded as significant pathogens were identified by conventional bacteriological tests; 399 strains isolated from 356 specimens submitted from 332 patients were studied and most were readily identified by the results of a combined set of morphological, biochemical, tolerance and antibiotic disk resistance tests. B. fragilis has particular pathogenic potential and was the commonest species isolated, accounting for > 50 % of strains. The next commonest was B. asaccharolyticus with 55 strains, and 16 other species or groups were represented by smaller numbers. Many (68 %) were from infections related to the gastro-intestinal tract, but there were significant numbers from infections of the male and female genito-urinary tracts, the head, neck and central nervous system and from a variety of soft tissue infections. Most infections were mixed, and a pure culture of a Bacteroides sp. was obtained from only 26 % of infections; two or more strains of Bacteroides were recovered from 55 infections. The specific identification of Bacteroides may help the bacteriologist to judge the significance of laboratory findings, influence the patient's management and prognosis and help determine the source of infection.

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“…Improvements in anaerobic techniques (Collee, Rutter and Watt, 1971;Holdeman and Moore, 1973;Watt, 1973;Watt, Hoare and Collee, 1973;Watt, Collee and Brown, 1974) have provided routine diagnostic bacteriological laboratories with reliable methods for the isolation of bacteroides organisms from a wide variety of clinical conditions, but few attempts are generally made to identify the isolates; they are usually reported as "Bacteroides spp. ", or at most the non-pigmented penicillin-resistant strains are reported as B. fragilis, the pigmented ones as B. melaninogenicus and the others as Bacteroides spp.…”

Section: Discussionmentioning

confidence: 99%