Some Ultrastructural Features of the Muscular Coat of Human Small Intestine

Pellegrini, Maria Simonetta Faussone; Cortesini, C

doi:10.1159/000145676

Cited by 50 publications

(12 citation statements)

References 20 publications

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“…Immunostaining for ChAT (1), measurement of released ACh (2) and nicotine-induced responses (3) were examined. Komuro, 1992;Komuro and Seki, 1995;Wang et al, 1999;Kobilo et al, 2003) and human (Faussone-Pellegrini, 1983;Torihashi et al, 1999;Wang et al, 2003) small intestine. Specialized contacts between ICC-IM and the neurons were also reported in the murine gastric fundus and antrum (Mitsui and Komuro, 2002), and colon .…”

Section: Discussionmentioning

confidence: 99%

Examination of the role of cholinergic myenteric neurons with the impairment of neural reflexes in the ileum of c-kit mutant mice

Okishio

Takeuchi

Fujita

et al. 2005

J. Smooth Muscle Res.

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Our previous study showed that impairment of ascending and descending neural reflexes in the ileum of the c-kit mutant, W/W V , mice is due to a loss of interstitial cells of Cajal present at the myenteric plexus region (ICC-MY) in the mutant. In the present study, cholinergic interneurons were thought to be involved in these pathways, since hexamethonium, an antagonist of the nicotinic ACh receptor, significantly inhibited both neural reflexes in wild type mice. Therefore, we examined whether the loss of ICC-MY affects cholinergic interneurons involved in these pathways. Immunohistochemistry with anti-choline acetyltransferase revealed that there was no difference in the numbers of immunopositive cells in the myenteric plexus region between the wild type and mutant mice. In addition, there was no difference in the extent of spontaneous and EFS-evoked ACh release from longitudinal muscle with myenteric plexus preparations between the wild type and mutant mice. Exogenously added nicotine induced contraction or relaxation of ileal circular muscle in the absence or presence of atropine, respectively, to a similar extent in both the wild type and mutant mice. These results suggest that loss of ICC-MY resulted in an impairment of the ascending and descending reflex pathways at the step before activation of cholinergic interneurons.

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Section: Discussionmentioning

confidence: 99%

Examination of the role of cholinergic myenteric neurons with the impairment of neural reflexes in the ileum of c-kit mutant mice

Okishio

Takeuchi

Fujita

et al. 2005

J. Smooth Muscle Res.

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“…1911Li, 1940;Silva, 1971;Gabel la, 1972Gabel la, . 1974Duchon et al, 1973;Thuneberg, 1982;Faussone Pellegrini, 1982Faussone Pellegrini and Cortesini, 1983]; however, the colonic PMP differs from the ileal PMP because ICC are absent.…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural Peculiarities of the Inner Portion of the Circular Layer of the Colon. II.

Pellegrini¹

1985

Cells Tissues Organs

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A layer of muscle cells consisting of 1–3 rows is present on the inner part of the circular layer of mouse colon. These muscle cells are thinner, with a denser cytoplasm and more caveolae than those of the main portion of the circular layer. A connective interstice divides the two parts of the circular layer and is occupied by a nerve plexus rich in varicosities. Groups of cells, identified as interstitial cells of Cajal, and a nerve plexus rich in varicosities are located on the border between the innermost circular layer and the tela submucosa. The interstitial cells have many cytological features in common with muscle cells (caveolae, basal lamina, thin filaments, smooth endoplasmic reticulum, glycogen particles) and touch each other and the nerve endings frequently and the muscle cells of the innermost layer rarely.

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“…In 1987, Saul et al 5 called for further immunohistochemical clarification of a possible cell of origin for these tumors, namely, the interstitial cell of Cajal (ICC), which has a pace-making function in the bowel. 21,22 A marker for these cells is c-kit (CD117), which is a transmembrane protein belonging to the receptor tyrosine kinase family and is also a marker of progenitor cells in bone marrow. 23,24 One interesting observation is that the majority of bone-marrow cells which are positive for c-kit also coexpress CD34.…”

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confidence: 99%

Diagnosis of gastrointestinal stromal tumor by fine-needle aspiration biopsy: A cytological and immunocytochemical study

Seidal

Edvardsson

2000

Diagn. Cytopathol.

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The diagnosis of gastrointestinal stromal tumors (GIST) is generally established on histopathologic examination of surgical specimens. The present study was undertaken to characterize the cytologic appearance of these tumors and to assess the utility of fine‐needle aspirates, including immunocytochemistry for the demonstration of c‐kit (CD117) and CD34, in the preoperative diagnosis. The study comprised preoperative fine‐needle aspirates in three cases and aspirates performed on fresh surgical specimens in eight cases. The routinely stained smears revealed a mesenchymal cell population, predominated by spindle cells. In five of six cases a strong positivity for c‐kit was demonstrated and in three of five cases a strong CD34‐expression was found in the tumor cells. These results show that fine‐needle aspiration appears to be a useful method for the recognition and diagnosis of GISTs or ICC (interstitial cell of Cajal) tumors. Diagn. Cytopathol. 2000;23:397–401. © 2000 Wiley‐Liss, Inc.

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Some Ultrastructural Features of the Muscular Coat of Human Small Intestine

Cited by 50 publications

References 20 publications

Examination of the role of cholinergic myenteric neurons with the impairment of neural reflexes in the ileum of c-kit mutant mice

Examination of the role of cholinergic myenteric neurons with the impairment of neural reflexes in the ileum of c-kit mutant mice

Ultrastructural Peculiarities of the Inner Portion of the Circular Layer of the Colon. II.

Diagnosis of gastrointestinal stromal tumor by fine-needle aspiration biopsy: A cytological and immunocytochemical study

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