Ultrastructural Peculiarities of the Inner Portion of the Circular Layer of the Colon. II.

Pellegrini, Maria Simonetta Faussone

doi:10.1159/000146000

Cited by 25 publications

(14 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…9) has been documented previously in the mouse (Faussone-Pellegrini 1985, 1987a, 1987bRumessen 1994) and the present study confirms these descriptions. X-gal deposits in ICC-SMP were not always seen, and they were often weak (Fig.…”

Section: Icc-smpsupporting

confidence: 93%

Distribution and ultrastructure of interstitial cells of Cajal in the mouse colon, using antibodies to Kit and Kit W-lacZ mice

Vanderwinden

Rumessen

Bernex

et al. 2000

Cell and Tissue Research

View full text Add to dashboard Cite

The roles of the interstitial cells of Cajal in the stomach and intestine are becoming increasingly clear. Interstitial cells of Cajal in the colon are less well known, however. We studied the development and distribution of the interstitial cells of Cajal in the mouse colon, using the tyrosine kinase receptor Kit as a marker. Sections and whole mounts were studied by confocal microscopy after double immunofluorescence with specific antibodies. The ultrastructure of Kit-expressing cells was examined by electron microcopy in KitW-lacz/+ transgenic mice, which carry the lacz gene inserted in place of the first exon of the Kit gene. In the subserosa, the interstitial cells of Cajal formed a two-dimensional plexus. In the myenteric area, the interstitial cells of Cajal formed a dense plexus that gradually merged with the interstitial cells of Cajal in the outer half of the circular muscle. The inner half of the circular layer was devoid of interstitial cells of Cajal whereas in the submuscular region the interstitial cells of Cajal formed a two-dimensional plexus. Tertiary nerves with various chemical codings closely followed interstitial cell of Cajal processes. By electron microscopy, Kit-expressing cells in the outer parts of the musculature had scattered caveolae, inconspicuous basal lamina and numerous mitochondria, whereas in the submuscular region they had more pronounced myoid features. Kit-expressing cells in the mouse colon are identifiable as interstitial cells of Cajal by their ultrastructure. The interstitial cells of Cajal in the mouse colon mature postnatally. They are organized into a characteristic plexus, close to the nerves with various chemical codings.

show abstract

Section: Icc-smpsupporting

confidence: 93%

Distribution and ultrastructure of interstitial cells of Cajal in the mouse colon, using antibodies to Kit and Kit W-lacZ mice

Vanderwinden

Rumessen

Bernex

et al. 2000

Cell and Tissue Research

View full text Add to dashboard Cite

show abstract

“…This finding further confirms that the darkly stained cells are the same as those previously identified by fluorescence and brightfield microscopy. The ultrastructure of the labeled cells was maintained and identical to that found previously for submucosal ICCs in the mouse colon (Faussone-Pellegrini 1985, 1987. These cells are elongated, rich with caveolae and mitochondria, and have a basal lamina.…”

Section: Discussionsupporting

confidence: 82%

“…Because of their location at the border between the submucosa and the circular muscle layer, they correspond to the submucosal ICCs of the mouse colon (Faussone-Pellegrini 1985, 1987.…”

Section: Discussionmentioning

confidence: 99%

Visualization of interstitial cells of Cajal in the mouse colon by vital staining

Hanani

Louzon

Miller

et al. 1998

Cell and Tissue Research

View full text Add to dashboard Cite

Interstitial cells of Cajal (ICCs) are believed to be a major element in generating the spontaneous rhythm of the gastrointestinal tract. A prominent problem in the study of these cells has been the difficulty in observing them in intact tissues. We used the lipophilic dye DiI to stain ICCs in the submucosal-circular muscle border of freshly dissected mouse colon. The placement of small DiI crystals in this area resulted in the labeling of ICC-like cells. Two main morphological cell types, viz., bipolar and multipolar, were noted. Bipolar cells had two primary processes emerging from the poles of an elongated soma. The mean length of these processes was 78.7 microm. These cells constituted 42.3% of the sample (n=105). Multipolar cells (54.3% of total) had a less elongated soma and extended 3-6 main processes whose mean length was 56.3 microm. These processes showed no preferred direction. The length of the primary processes of bipolar cells was 40% greater than that of multipolar cells (P<0.02). Three cells (2.9%) had only one primary process. The DiI stain could be converted into a stable electron-opaque product. Electron-microscopic observations showed that these cells had the typical appearance of ICCs reported in previous studies. This staining method should be useful for physiological investigations of ICCs in gastrointestinal tissues.

show abstract

“…Rhodamine 123 is a cationic dye that is thought to be concentrated in mitochondria by the relatively high negative potential across the energized mitochondrial membrane (Johnson et al 1981). We reasoned that rhodamine 123 might be used to label ICC, because these cells have a relative abundance of mitochondria (Thuneberg 1982;Faussone-Pellegrini 1985, 1987Ward and Sanders 1990). Our study did not specifically determine the mechanism by which ICC concentrate rhodamine 123, but the data suggest that the dye discriminates well between ICC and smooth muscle cells.…”

Section: Discussionmentioning

confidence: 99%

“…These similarities increase the difficulty of designing specific methods to identify and/or to specifically lesion the function of ICC in situ. One striking morphological difference between ICC and smooth muscle cells is that ICC 216 contain an abundance of mitochondria (Thuneberg 1982;Faussone-Pellegrini 1985, 1987Ward and Sanders 1990). We reasoned that the large number of mitochondria in ICC might provide a means to label and lesion these cells.…”

Section: Introductionmentioning

confidence: 99%

Use of rhodamine 123 to label and lesion interstitial cells of Cajal in canine colonic circular muscle

1990

View full text Add to dashboard Cite

The role of interstitial cells of Cajal (ICC) is difficult to determine because these cells are not easily identified by light microscopy, and there are no compounds available to specifically lesion ICC. Ultrastructural studies have shown an abundance of mitochondria in ICC. Therefore, we have used rhodamine 123, a fluorescent dye that is specifically accumulated by mitochondria, to identify ICC in canine proximal colon. This technique provided good discrimination between ICC and smooth muscle cells, but enteric neurons were labeled with rhodamine 123. This compound has cytotoxic properties in some cells. Therefore, we treated intact muscle strips with rhodamine 123 while recording intracellular electrical activity from circular muscle cells. Uptake of rhodamine 123 by ICC was associated with an alteration in electrical rhythmicity. These data suggest that rhodamine 123 may be a useful tool for visualizing and perhaps chemically lesioning ICC.

show abstract

Ultrastructural Peculiarities of the Inner Portion of the Circular Layer of the Colon. II.

Cited by 25 publications

References 2 publications

Distribution and ultrastructure of interstitial cells of Cajal in the mouse colon, using antibodies to Kit and Kit W-lacZ mice

Distribution and ultrastructure of interstitial cells of Cajal in the mouse colon, using antibodies to Kit and Kit W-lacZ mice

Visualization of interstitial cells of Cajal in the mouse colon by vital staining

Use of rhodamine 123 to label and lesion interstitial cells of Cajal in canine colonic circular muscle

Contact Info

Product

Resources

About