Some Physicochemical Characteristics of an Immune Lymphocyte Product Which Inhibits the Multiplication of Toxoplasma within Mouse Macrophages

Shirahata, Toshikazu; Shimizu, Kiheiji

doi:10.1111/j.1348-0421.1979.tb00436.x

Cited by 11 publications

(6 citation statements)

References 29 publications

(32 reference statements)

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“…The lymphoid cells had to be specific for both host and microbe. Specificity of induction has also been observed for the T. gondii growth inhibitory factor (19).…”

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confidence: 75%

“…We have demonstrated in hamsters (4) that kidney cells and fibroblasts infected with T. gondii can be protected by both lymphocytes from immune animals and a mediator which appears in the supernatant of immune lymphocytes in contact with specific antigen. Previous studies have concentrated on peritoneal macrophages because they are readily obtained and because appreciable killing of T. gondii is readily observed (1,11,14,17,19,20). In addition, because of their phagocytosis of T. gondii, macrophages have been thought to play an important role in hose defense.…”

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confidence: 99%

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Specific mediation of cellular immunity to Toxoplasma gondii in somatic cells of mice

Chinchílla¹,

Frenkel²

1984

Infect Immun

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Lymphocytes from mice immunized against Toxoplasma gondii protected T. gondii-infected macrophage and kidney cell cultures. After contact with antigens, supernatants of such immune lymphocytes, also contained a factor protective for T. gondii-infected macrophages and kidney cells. Supernatants were protective only when the lymphocytes and kidneys cells were isogeneic. Protection was specific in that supernatants from only T. gondii-immune, but not Besnoitia jellisoni-immune, lymphocytes provided protection against toxoplasmosis. Sixteen to 24 h were required for an appreciable amount of protective factor to be secreted; a similar absorption time was necessary for kidney cells to be protected. Peritoneal lymphocyte lysates, prepared as transfer factor, contained protective substances with a potency similar to that of lymphocyte supernatants, which were also strain restricted in their effect.

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“…The lymphoid cells had to be specific for both host and microbe. Specificity of induction has also been observed for the T. gondii growth inhibitory factor (19).…”

mentioning

confidence: 75%

mentioning

confidence: 99%

Specific mediation of cellular immunity to Toxoplasma gondii in somatic cells of mice

Chinchílla¹,

Frenkel²

1984

Infect Immun

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“…The active Sephadex G-100 fraction was concentrated by ultrafiltration and dialyzed overnight against 0.002 M PB (pH 7.2). Isoelectric focusing was performed at 2 C in a 110-m1 isoelectric focusing column (KatoShoten, Co., Ltd., Osaka) as described previously (23). After the power was turned.…”

Section: Methodsmentioning

confidence: 99%

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confidence: 99%

“…W enamed this factor Toxoplasma growth inhibitory factor (Toxo-GIF) (24). Partial characteraization of Toxo-GIF revealed that there are many similarities between Toxo-GIF and mouse immune interferon (IF) in such physicochemical and biological properties as molecular weight, lability at pH 2 and in trypsin, thermostability and species specificity (14,23,26). In 1966, Rytel and Jones (18) and Freshman et al (6) first reported the induction and partial characterization of circulating IF by a protozoan infection, in mice inoculated with a virulent strain of Toxoplasma.…”

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confidence: 99%

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Production and Properties of Immune Interferon from Spleen Cell Cultures of Toxoplasma‐Infected Mice

Shirahata

Shimizu

1980

Microbiology and Immunology

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In response to antigenic stimulation, spleen cells from Toxoplasmainfected mice produce a factor showing inhibitory activity against vesicular stomatitis virus infection in L cell cultures. When BALB/C and ICR mice were inoculated intraperitoneally with the low-virulent S-273 strain of T. gondii, such activity was first detected in 4 and 7 days and reached maximum levels at 10 and 14 days respectively, and retained these levels for at least three weeks. However, BALB/C mice, which are considerably more sensitive to Toxoplasma infection than ICR mice, produced significantly smaller amounts of interferon (IF) after challenge with the high virulent strain.The IF produced in this system possessed certain known properties of immune (type II) IF and was not neutralized by rabbit antiserum against mouse type I IF. The immune IF preparation also inhibited multiplication of Toxoplasma within nonphagocytic L cells in an IF-like fashion, whereas Newcastle disease virusinduced (type I) IF had no effect on this parasite. The antiviral and anti-Toxoplasma activity in immune IF preparations could not be distinguished solely on the bases of their molecular weight and isoelectric point. The experiments with anti-0 serum plus complement and with nylon wool column effluent cells strongly suggest that immune IF was produced by T lymphocytes and required the assistance of macrophages.

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Toxoplasmosis: The Need for Improved Diagnostic Techniques and Accurate Risk Assessment

Hughes

1985

Current Topics in Microbiology and Immunology

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Some Physicochemical Characteristics of an Immune Lymphocyte Product Which Inhibits the Multiplication of Toxoplasma within Mouse Macrophages

Abstract: In response to antigenic stimulation, the splenic lymphocytes from

Cited by 11 publications

References 29 publications

Specific mediation of cellular immunity to Toxoplasma gondii in somatic cells of mice

Specific mediation of cellular immunity to Toxoplasma gondii in somatic cells of mice

Production and Properties of Immune Interferon from Spleen Cell Cultures of Toxoplasma‐Infected Mice

Toxoplasmosis: The Need for Improved Diagnostic Techniques and Accurate Risk Assessment

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