Some physicochemical and antinutritional properties of raw flours and protein isolates from Mucuna pruriens (velvet bean) and Canavalia ensiformis (jack bean)

Abstract

The legumes Canavalia ensiformis and Mucuna pruriens are underexploited in tropical Mexico. Their seeds have good nutritional potential, but contain antinutritional factors. Physicochemical and antinutritional properties were determined for raw flours (RF) and protein isolates (PI) produced from these legumes. Protein content in the PI was 737 g kg -1 for C. ensiformis and 666 g kg -1 for M. pruriens. Protein isolation improved in vitro digestibility, while maintaining high lysine levels and adequate sulphur amino acids content. Antinutritional factors such as cyanogenic glucosides, cyanide precursors from hydrolysis, tannins and trypsin inhibitors were lower in the PIs than in the RFs. The reduction in canavanine levels, a structural analogue of arginine, in the C. ensiformis PI was noteworthy. These PIs thus have potential applications in the development of new food ingredients in tropical regions using processes that improve nutritional value.

Extraction, purification and properties of trypsin inhibitor from Thai mung bean (Vigna radiata (L.) R. Wilczek)

“…Trypsin and chymotrypsin inhibitory proteins have been isolated and characterised from a variety of legume seeds, e.g. pigeon pea (Godbole, Krishna, & Bhatia, 1994), tepary bean (Campos, Martinez-Gallardo, Mendiola-Olaya, & Blanco-Labra, 1997), cowpea, bambara groundnuts (Benjakul et al, 2000), mung bean seed (Phaseolus mungo) (Wang et al, 2006), Australian wattle seed (Acacia victoriae Bentham) (Ee, Zhao, Rehman, & Agboola, 2008), dry bean (Nergiz & Gokgoz, 2007), velvet bean and jack bean (Betancur-Ancona et al, 2008). Recently, Klomklao, Benjakul, Kishimura, Osako, and Tanaka (2010a) reported that adzuki bean showed high trypsin inhibitory activity.…”

A heat‐stable trypsin inhibitor in adzuki bean (Vigna angularis): effect of extraction media, purification and biochemical characteristics

“…Trypsin and chymotrypsin inhibitory proteins have been isolated and characterised from a variety of legume seeds, e.g. pigeon pea (Godbole et al, 1994), tepary bean (Campos et al, 1997), cowpea, bambara groundnuts (Benjakul et al, 2000), dry bean (Nergiz & Gokgoz, 2007), velvet bean and jack bean (Betancur-Ancona et al, 2008). Based on our preliminary study, adzuki bean seeds contained high trypsin inhibitory activity.…”

“…, 1997), cowpea, bambara groundnuts (Benjakul et al. , 2000), dry bean (Nergiz & Gokgoz, 2007), velvet bean and jack bean (Betancur‐Ancona et al. , 2008).…”

Proximate, Mineral and Anti-nutrient Composition of Pumpkin (<i>Cucurbitapepo L)</i> Seeds Extract

“…Only p lants with mo re than 200 mg of hydrocyanic acid equivalent per 100 mg fresh weight are considered dangerous [23]. Consumption of high levels of Cyanide is associated with a serious health problem, a neurological disease known as Tropical Ataxis Neu rophathy (TAN) was lin ked to consumption of high level of cyanide in cassava based diet [24].…”