Some pH Changes in Nutrient Solutions

Vacin, Emil F.; Went, F. W.

doi:10.1086/335561

Cited by 371 publications

(161 citation statements)

References 0 publications

Supporting

Mentioning

140

Contrasting

Unclassified

Order By: Relevance

“…For this purpose, liquid VW (Vacin and Went, 1949) and New Dogashima medium (NDM) (Tokuhara and Mii 1993; were used and each liquid medium was supplemented with 20% coconut water and different concentrations of chitosan (0,5,10,15,20 and 25 mg/L). Chitosan powder labeled as low molecular weight and 75% degree of deacetylation (Sigma Aldrich) was selected for the preparation of stock solution.…”

Section: Media and Chitosan Preparationmentioning

confidence: 99%

Genetic Stability of In vitro Multiplied Phalaenopsis gigantea Protocorm-like Bodies as Affected by Chitosan

Samarfard¹,

Kadir²,

Kadzimin³

et al. 2013

Not Bot Hort Agrobot Cluj

View full text Add to dashboard Cite

Chitosan is a carbohydrate polymer derivative of chitin which presents in shell of crustaceans. This biopolymer is a non toxic and environmentally friendly, considered as a plant growth stimulator in some plant species. The present study investigates the effects of chitosan and media types on multiplication and genetic stability of Phalaenopsis gigantea protocorm-like bodies (PLBs). PLBs were inoculated in liquid New Dogashima Medium (NDM) and Vacin and Went (VW) supplemented with various concentrations of chitosan (0, 5, 10, 15, 20 and 25 mg/L). The highest PLB multiplication was observed on VW and NDM supplemented with 10 mg/L chitosan with mean number of PLBs 177 and 147, respectively. Chitosan promoted the formation of juvenile leaves and the highest number was observed in NDM supplemented with 20 mg/L chitosan with mean number of 66 leaves after 8 weeks of culture. Genetic stability was assessed among mother plant and secondary PLBs after 2, 4, 6, and 8 weeks of culture in liquid media. 8 out of 10 ISSR markers produced a total of 275 clear and reproducible bands with mean of 6.9 bands per primer. The secondary PLBs produced during sub-culturing process of chitosan treated liquid culture were genetically uniform and similar to mother plant.

show abstract

Section: Media and Chitosan Preparationmentioning

confidence: 99%

Genetic Stability of In vitro Multiplied Phalaenopsis gigantea Protocorm-like Bodies as Affected by Chitosan

Samarfard¹,

Kadir²,

Kadzimin³

et al. 2013

Not Bot Hort Agrobot Cluj

View full text Add to dashboard Cite

show abstract

“…Half strength MS (Murashige and Skoog 1962), MS, KC (Knudson 1922) and VW (Vacin and Went 1949) media were used for preliminary experiment to evaluate suitable medium for seed germination. After preliminary experiment, MS medium was found most suitable and was used for all further experiments.…”

Section: Culture Initiation and Mediamentioning

confidence: 99%

Asymbiotic seed germination and in vitro conservation of Coelogyne nervosa A. Rich. an endemic orchid to Western Ghats

Abraham

Augustine

Thomas

2012

Physiol Mol Biol Plants

View full text Add to dashboard Cite

Coelogyne nervosa is an epiphytic orchid endemic to Western Ghats, South India. The mature seeds of C. nervosa were cultured on ½ MS (Murashige and Skoog), MS, Kn (Knudson) and VW (Vacin and Went) media to evaluate the seed germination response. Of the four basal media used, MS medium supported maximum seed germination. Further experiments to enhance seed germination were done on MS medium supplemented with various concentrations (10, 20, 30 and 40 %) of coconut water (CW). Thirty percent CW gave the highest response in terms of percent seed germination (96), fresh weight (7.2 mg/seedling) and protocorm length (15.2 mm). Since CW containing medium did not support further seedling growth, each seedling was isolated and cultured on MS medium supplemented with either BA (6-benzylaminopurine) or Kin (kinetin) alone (1.0-4.0 mg/l each) or in combination with NAA (1-naphthaleneacetic acid; 0.2-1.0 mg/l). Maximum growth was observed on MS medium supplemented with BA (3.0 mg/l) and NAA (0.5 mg/l). On this medium, the seedlings reached an average length of 3.6 cm with 2.8 well expanded green leaves per seedling. Similarly, optimum, healthy, white root induction (3.3 roots/seedlings) was also observed on the same medium. The rooted seedlings were successfully transplanted to pots with 91 % success.The 2-year-old tissue culture derived plants produced normal flowers and fruits.

show abstract

“…O meio de cultura é essencial para os desenvolvimentos vegetativo e radicular, podendo conter diferentes combinações de nutrientes, de acordo com os requerimentos de cada espécie (Faria et al, 2002). Dentre os meios de cultura mais utilizados para a propagação in vitro, destacam-se os meios MS (Murashige & Skoog, 1962), Knudson C (Knudson, 1946) e Vacin e Went (Vacin & Went, 1949).…”

Section: Introductionunclassified

Fertilizantes comerciais e polpa de banana no cultivo in vitro de um híbrido de Phalaenopsis (Orchidaceae)

2012

View full text Add to dashboard Cite

Commercial fertilizers and banana pulp for in vitro cultivation of a Phalaenopsis hybridThe in vitro propagation of orchids is a largely used procedure for the production of seedlings. The search for alternative culture media with this purpose has been widely studied due to the complexity of the commonly used media, e.g. the MS medium. The hybrids of Phalaenopsis are among the most commercialized orchids in the world due to the longevity and distinct beauty of its flowers. The objective of this study was to evaluate the effect of commercial fertilizers' formulations and the addition of 'Nanica' banana pulp in a culture medium for in vitro cultivation of a hybrid of Phalaenopsis. Seedlings germinated in vitro, in MS medium, were subcultivated in culture media with a basis of commercial fertilizers and MS medium modified with half the concentration of macronutrients. The culture media were evaluated with and without the addition of the banana pulp (100g L -1 ) during the stage of maturation 4. The basis of these culture media was composed of sucrose (30g L -1 ), activated charcoal (1g L -1 ) and agar (9g L -1 ). After 180 days the following variables were analyzed: leaf area, number of leaves and roots, root length and dry weight of leaves and roots. We were able to conclude that the treatment composed by Biofert® supplemented with banana pulp presented the best results for the hybrid development in vitro, and actually presented statistically superior results in relation to MS without banana.

show abstract

Some pH Changes in Nutrient Solutions

Cited by 371 publications

References 0 publications

Genetic Stability of In vitro Multiplied Phalaenopsis gigantea Protocorm-like Bodies as Affected by Chitosan

Genetic Stability of In vitro Multiplied Phalaenopsis gigantea Protocorm-like Bodies as Affected by Chitosan

Asymbiotic seed germination and in vitro conservation of Coelogyne nervosa A. Rich. an endemic orchid to Western Ghats

Fertilizantes comerciais e polpa de banana no cultivo in vitro de um híbrido de Phalaenopsis (Orchidaceae)

Contact Info

Product

Resources

About