SOME OF BIOCHEMICAL CHANGES INDUCED BY THEOPHYLLINE AND FUROSEMIDE IN THE LAND SNAIL, Monacha obstructa

Abstract

The biochemical effects of two compounds i.e. theophylline and furosemide were studied on the glassy clover snail Monacha obstructa. Animals were treated with sub-lethal concentration (¼ LC50) of each tested compound using contact (thin film) technique. Some biochemical parameters were measures at different periods after treatment. Results showed that the two tested compounds gradually reduced the activity of aspartate amino transferase (AST) and peroxidase enzymes with the prolongation of periods after treatment, while the contrary occurred with alanine aminotransferase (ALT) as both compounds enhanced its activity proportionally than control. Concerning lactic acid dehydrogenase (LDH), results showed severe reduction in its level after 1, 2, 3 and 4 days post-treatment. Also catalase enzyme took adverse way as its activity increased in the 1 st , 2 nd , 3 rd and 4 th days for both compounds. On the other hand, levels of the total protein and total lipid were significantly or insignificantly decreased post-treatment with the two tested compounds.

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“…Findings similar to this study were also obtained in a study in rats given 20mg/kg /day theophylline [35]. Khidr et al [37] found that catalase enzymes increased on day 4, while the study of snails showed a similar decrease in AST, ALT and LDH levels in the group of theophylline. For 3 days during the work we did i.v.…”

“…Edmunds & Mayhew [16] also reported that theophylline use at high doses may lead to proteinuria. Bruguerolle & Dubus [39] claimed that high fever caused an increase in blood protein and albumin levels and that theophylline administration did not cause a change in TP and ALB levels in high-fever subjects [37], in their study of snails, causing them to decrease slightly.…”