Some Long‐Term Cell Kinetic Effects of Ionizing Radiation On Mouse Bladder Urothelium

Reitan, Jon B.

doi:10.1111/j.1365-2184.1986.tb00750.x

Cited by 2 publications

(2 citation statements)

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Section: Discussionmentioning

confidence: 99%

“…10 Reitan reported that urothelium has low compensatory capacity irradiation, because of a recovery period of about 3 months following 10 Gy and approximately a 9-month period following 20 Gy. 17 The mechanism of radiation damage in the urinary bladder is clearly different from tissues with more rapid turnover times. Stewart et al showed that the urothelium of mice at 2 weeks after 30 Gy was intact, with large superficial cells lining the luminal surface of the bladder, whereas the first histological changes were detectable at about 5 months.…”

Section: Discussionmentioning

confidence: 99%

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POMC expression of the urothelium of the urinary bladder of mice submitted to pelvic radiation

et al. 2016

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Objective: Patients who have had pelvic radiotherapy as part of their cancer therapy may develop subsequent urinary bladder injury. The acute changes that the urothelium undergo after radiation are known, but the healing mechanism of the urothelium of the urinary bladder after pelvic radiotherapy is not clearly understood. Proopiomelanocortin (POMC) peptides, which have immunomodulatory effects, are produced locally in sites outside of the central nervous system. This study aims to determine the role of POMC expression in the urothelium during radiation injury. Methods: Twenty-four male Swiss Albino mice were divided into four groups. A single-fractioned 10 Gy of ionizing radiation was applied to the pelvic zone of all mice with Cobalt-60 radiotherapy. The first group 1, which consisted intact animal and not irradiated was the control group, and the second, third, and fourth groups were euthanized after 24 h (Group 2), 48 h (Group 3), and 7 days (Group 4) after irradiation. All bladders were prepared for histochemical analysis using hematoxylin eosin (H&E) and immunohistochemical analysis using anti-POMC antibody. Results: No morphological differences were seen in all the group samples stained with H&E. POMC expression of the urothelium of bladder tissue samples shows different staining levels. Group 1 (96.7 ± 7.68), Group 2 (88.3 ± 8.04), and Group 3 (85.10 ± 10.9) were very weakly stained, but the POMC immunoreactivity of Group 4 (113.0 ± 12.8) was observed to be strong. Conclusion: Expression of POMC from urothelium seems to prevent bladder damage from radiation supplying differentiation and restoration of the urothelium.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%