Some Kinetic Properties of Lactate Dehydrogenase Activity in Cell Extracts from a Mammalian (Ovine) Corneal Epithelium

Doughty, Michael J.

doi:10.1006/exer.1997.0423

Cited by 9 publications

(3 citation statements)

References 27 publications

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“…[ 47 ] In fact, G6PDH that produces NADPH and induces coacervate formation exhibits high affinity for G6P and NADP + (K M values for G6P and NADP + are ≈2.0 × 10 −5 M and ≈2.0 × 10 −6 M, respectively), [ 48 ] while LDH involved in the consumption has relatively low affinity for NADPH (≈4.55 × 10 −3 M) and high affinity toward pyruvate (K M value ≈ 2.0 × 10 −5 M). [ 48‐51 ] This is consistent with our observation that the NADPH‐coacervate dissolution behavior is more sensitive to the changes in pyruvate concentration compared to changes in that of LDH. [ 52,53 ] Moreover, considering that our enzymatic network design incorporates two competing enzymes, LDH and G6PDH, the substantial difference in affinity for NADPH and NADP + may further amplify this tendency; the three orders of magnitude difference in affinity of G6PDH for NADP + compared to LDH for NADPH suggests a significantly faster NADPH production rate than consumption.…”

Section: Resultssupporting

confidence: 89%

Programmable Enzymatic Reaction Network in Artificial Cell‐Like Polymersomes

Seo,

Lee

2024

Advanced Science

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The ability to precisely control in vitro enzymatic reactions in synthetic cells plays a crucial role in the bottom‐up design of artificial cell models that can recapitulate the key cellular features and functions such as metabolism. However, integration of enzymatic reactions has been limited to bulk or microfluidic emulsions without a membrane, lacking the ability to design more sophisticated higher‐order artificial cell communities for reconstituting spatiotemporal biological information at multiple length scales. Herein, droplet microfluidics is utilized to synthesize artificial cell‐like polymersomes with distinct molecular permeability for spatiotemporal control of enzymatic reactions driven by external signals and fuels. The presence of a competing reverse enzymatic reaction that depletes the active substrates is shown to enable demonstration of fuel‐driven formation of sub‐microcompartments within polymersomes as well as realization of out‐of‐equilibrium systems. In addition, the different permeability characteristics of polymersome membranes are exploited to successfully construct a programmable enzymatic reaction network that mimics cellular communication within a heterogeneous cell community through selective molecular transport.

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Section: Resultssupporting

confidence: 89%

Programmable Enzymatic Reaction Network in Artificial Cell‐Like Polymersomes

Seo,

Lee

2024

Advanced Science

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“…0.03 µmol s -1 mg -1 LDH. Although somewhat lower than values reported in the literature [20,29], the estimate for vmax on the CP-MWCNT support appear to be of an acceptable agreement, considering the particular 3-dimensional nanometric support.…”

Section: Electroless Enzymatic Testscontrasting

confidence: 41%

Modelling of a hybrid bioelectrocatalytic flow reactor with NADH cofactor regeneration by immobilized rhodium mediator for pyruvate bioconversion

Housseini

Etienne

Lojou

et al. 2023

Chemical Engineering and Processing - Process Intensification

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“…Thus, LDH was measured to assess the role of conjunctival epithelium in corneal regeneration (Thoft and Friend, 1977), and was correlated with energy needs for epithelial migration in the healing of corneal wounds (Kuwabara, Perkins and Cogan, 1976). LDH has also been used as a marker of cell integrity in the corneal stroma and epithelium (Doughty, 1997;1998). However, a direct association between corneal epithelial cell differentiation and LDH expression has not been demonstrated.…”

Section: Discussionmentioning

confidence: 99%