Some hydrolases in human schistosomiasis with special reference to arylsulfatase B

Balbaa, Mahmoud; Abbas, Sahar E.; El-Deen, Seham N.; Hassab, Amina; Awad, Olfat

doi:10.1080/0020723032000069196

Cited by 4 publications

(5 citation statements)

References 22 publications

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“…Moreover, infection with Schistosoma mansoni resulted in a disturbance in the nucleic acid metabolism in the livers of infected mice (Hunter et al, 1973). Recently, it has been found that lipase, α-amylase, and leucine aminopeptidase displayed a significant increase in human serum during schistosomiasis (Balbaa et al, 2003). It was reported that the induction of nitric oxide synthase activity in murine schistosomiasis lead to deleterious effects on the liver (Abdallahi et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

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Activity of Some Hepatic Enzymes in Schistosomiasis and Concomitant Alteration of Arylsulfatase B

Balbaa

Elkersh²,

Mansour³

et al. 2004

BMB Reports

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The levels of arylsulfatases A and B, α-amylase, aspartate transcarbamylase, and γ-glutamyl transpeptidase were investigated during the infection of mice with schistosoma mansoni. This infection caused a significant (p<0.001) increase in the activity of hepatic arylsulfatase B (ASB), aspartate transcarbamylases and γ-glutamyl transpeptidase. A non-significant difference occurred for α-amylase (p<0.3) and arylsulfatase A (p>0.5) when compared to the control. The specific activity of hepatic ASB was progressively increased with the progression of the Schistosoma-infection. Moreover, the kinetic studies of hepatic ASB in Schistosomainfection showed that a slight decrease in the value of K m and about a 40% increase in V max when compared to the control. In addition, the pH optimum of hepatic ASB was altered from 6 to 7 as a result of schistosomiasis. These observations suggest that there are schistosomiasis-associated changes of the catalytic and kinetic properties of hepatic ASB.

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Section: Discussionmentioning

confidence: 99%

“…This endemic disease affects the liver, intestine, spleen, and urinary bladder (Mellin et al, 1993). However, various enzyme changes were observed during Schistasoma mansoni, such as arylsulfatase B (Higuchi et al, 1984) and many hydrolases (Nabih and El-Ansary, 1991;Cesari et al, 2000;Balbaa et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Activity of Some Hepatic Enzymes in Schistosomiasis and Concomitant Alteration of Arylsulfatase B

Balbaa

Elkersh²,

Mansour³

et al. 2004

BMB Reports

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“…For the assay of the enzyme by catalyzing the oxidation of succinate by DCIP [25], the reaction mixture is the same as described above for Cyt c except that 5.0-50 μM DCIP was used instead of Cyt c. The decrease of absorbance at 600 nm (ΔA/s) was measured spectrophotometrically. The maximum velocity was expressed as nM/min/mg protein of SCR in catalyzing the reduction of both Cyt c and DCIP as described previously [3,5].…”

Section: Animal Infectionmentioning

confidence: 99%

“…The liver homogenates, which were prepared from livers of 15 mice from control and 20 mice from infected group were pooled and subjected to modified techniques of the previously described enzyme isolation and purification [3,5,16]. Briefly, the mitochondrial fraction was prepared for each 1 g of the mince ground in a mortar at 4.0°C for 2 h with 1.4 ml of 0.02 M sodium phosphate buffer, pH 7.4 containing 0.5% sodium cholate and 1.0 mM EDTA.…”

Section: Animal Infectionmentioning

confidence: 99%

Succinate cytochrome c reductase in schistosomiasis: in vitro inhibition by some schistosomicidal drugs

et al. 2010

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Enzymes in mitochondria play an important role in biological oxidation and energy production. To understand the effect of schistosomiasis on these important processes, succinate cytochrome c reductase (SCR) from control and Schistosoma-infected mice was subjected for investigation. In this article, we report that SCR from Schistosoma-infected mouse showed a significant decrease in its Vmax and Km compared to control using both cytochrome c and 2,6-dichlorophenolindophenol as substrates. Furthermore, the kinetic studies of the purified SCR in the absence and presence of the schistosomicidal drugs praziquantel and Commiphora extract reveal that both drugs have an inhibitory action on the enzyme from the control and Schistosoma-infected mice and praziquantel changes the type of inhibition of SCR towards cytochrome c from mixed type in control to a competitive one in the case of the infection.

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“…However, a change in various enzymes was observed during Schistasoma mansoni such as arylsulfatase B [9] and many hydrolases [10][11][12][13]. Arylsulfatases A, B and C (arylsulfo-hydrolases) are a group of hydrolytic enzymes that occur in various tissues and fluids [14].…”

Section: Introductionmentioning

confidence: 99%

In vitroeffect of schistosomicidal drugs on hepatic arylsulfatase B from theSchistosoma-infected mouse

Balbaa¹,

Bassiouny²

2006

Journal of Enzyme Inhibition and Medicinal Chemistry

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Arylsulfatase B (ASB) hydrolyzes the desulfation of N-acetylgalactosamine-4-sulfate at the non-reducing terminal of glycosaminoglycans. This enzyme activity was found to be elevated in mice schistosomiasis. In the present study, the catalytic and immunological properties of purified ASB from the liver of Schistosoma-infected mouse was investigated in the presence and absence of the schistosomicidal drugs praziquantel and Commiphora extract. The in vitro effect of praziquantel was found to be inhibitory with a Ki value of 5.5 x 10(-4) M while that of commiphora extract was as an activator. Furthermore, these drugs did not have an observed effect on the immunological properties of ASB with regard to its binding to its polyclonal rabbit antibody. These results indicate that some schistosomicidal drugs may reverse the alteration of the catalytic properties of the enzyme in schistosomiasis.

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Some hydrolases in human schistosomiasis with special reference to arylsulfatase B

Cited by 4 publications

References 22 publications

Activity of Some Hepatic Enzymes in Schistosomiasis and Concomitant Alteration of Arylsulfatase B

Activity of Some Hepatic Enzymes in Schistosomiasis and Concomitant Alteration of Arylsulfatase B

Succinate cytochrome c reductase in schistosomiasis: in vitro inhibition by some schistosomicidal drugs

In vitroeffect of schistosomicidal drugs on hepatic arylsulfatase B from theSchistosoma-infected mouse

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