Some effects of administering ergothioneine to rats

Heath, Harold; Rimington, C.; Searle, C. E.; Lawson, A. M.

doi:10.1042/bj0500530

Cited by 26 publications

(14 citation statements)

References 11 publications

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“…Despite previous results to the contrary [1,2,14], we found that ERT is taken up unchanged by human erythrocytes. The most likely reason for our ability to demonstrate such uptake was the use of a more specific assay for ERT than was used in the early studies.…”

Section: Discussioncontrasting

confidence: 99%

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Uptake and antioxidant effects of ergothioneine in human erythrocytes

Mitsuyama

May

1999

Clinical Science

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Ergothioneine is a fungal metabolite that may have antioxidant functions in mammalian cells. Although it accumulates to low millimolar concentrations in liver and other tissues, it is not thought to be taken up by mature erythrocytes. During a study of the function of ergothioneine as an antioxidant in human erythrocytes, we found that these cells do take up ergothioneine from the surrounding medium. Ergothioneine concentrations in freshly prepared erythrocytes were 2-9-fold higher than in plasma from the same donor. Slow but progressive accumulation of ergothioneine to about 125% of basal levels was observed in erythrocytes over a 4 h incubation. After a 2 h incubation, intracellular ergothioneine concentrations rose on addition of increasing amounts of ergothioneine to the incubation medium, although saturation was not evident in cells from all donors. Both initial levels and rates of ergothioneine uptake varied in erythrocytes from different donors. Intracellular ergothioneine was stable to depletion of GSH by N-ethylmaleimide and to a more severe oxidant stress induced by hydrogen peroxide in the presence of catalase. These results show that human erythrocytes do take up ergothioneine; however, the GSH results do not support an antioxidant role for ergothioneine in erythrocytes.

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Section: Discussioncontrasting

confidence: 99%

“…Since animals cannot synthesize ERT [1], there must exist mechanisms for its uptake and retention within cells. Early studies failed to demonstrate uptake of ERT into human erythrocytes following incubation for as long as 4 h [1,2]. However, there is controversy regarding whether or not ERT is taken up by erythrocytes.…”

Section: Introductionmentioning

confidence: 99%

Uptake and antioxidant effects of ergothioneine in human erythrocytes

Mitsuyama

May

1999

Clinical Science

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“…The origin and function ofergothioneine in blood are unknown; its presence, however, is well established (see Heath, Rimington, Searle & Lawson, 1952, for a general review). Until the discovery by Leone & Mann (1951) of ergothioneine in the seminal vesicle secretion of the boar, the only report of ergothioneine in animals was in blood cells.…”

Section: (Received 3 December 1952)mentioning

confidence: 99%

The metabolism of 35S-labelled 2-thiolhistidine and ergothioneine in the rat

Heath

1953

Biochemical Journal

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“…LE is known as an unique and single antioxidant amongst antioxidants which chelates heavy metals and protects of the cells (primarily erythrocytes) from ROS damage. Further, LE is shown as a single and powerful antioxidant which induces lipid peroxidation alike thiol antioksidants in the presence of ferric acid antioxidants and neutralizes the hydroxyl radicals, peroxynitrite hypochloroik acid and peroxynitrite [19][20][21] .…”

Section: Introductionmentioning

confidence: 99%

Koç Spermasının Dondurulması Üzerine L-Ergothionin, N-asetil sistein ve Sisteinin Etkileri

Yıldız¹,

Öztürkler²,

Ari³

et al. 2015

Kafkas Univ Vet Fak Derg

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The aim of this study was to determine the influence of different doses of L-Ergothioneine (LE), cystein and N-acetylcystein (NAC) on post-thawing semen parameters in rams. Ejaculates collected from four Tushin Rams were evaluated and pooled at 35°C. Semen samples were diluted with skim-milk based extender containing LE (5, 10 mM), Cystein (5, 10 mM), NAC (5, 10 mM) or not containing any antioxidant (control) and loaded to 0.25 ml French straws. Straws were cooled to 5°C for 2 h, frozen in liquid nitrogen vapour (aprox. -120°C) for 15 min and then being stored in liquid nitrogen until thawing process. Straws were thawed in water bath (37°C for 1 min). The percentages of motility, viability, abnormal acrosome, total abnormalities, membrane integrity (hypoosmatic swelling test, HOST) were statistically assessed. Also total antioxidant capacity (TAC) and total oxidatif stres (TOS) were evaluated in samples from replications. It was seen that LE was superior to N-acetylcystein and cystein in motility, viability, defected acrosome, total morphological abnormalities, HOST and TAC, (P<0.05) except cystein in motility. Neverthless, there was not any statistically difference between LE and control groups (P>0.05). It was conlcluded that there was not any beneficial or detrimental effects of LE on post thawing semen parameters in rams while it was determined that cystein and NAC may have been some detrimental effects on post thawing semen parameters in rams. These results warrants future scientific studies on LE, NAC and cystein in ram semen cryopreservation. Keywords: Ram semen, Cryopreservation, L-Ergothioneine, Cystein, N-acetylcystein, Tushin Koç Spermasının Dondurulması Üzerine L-Ergothionin, N-asetil sistein ve Sisteinin Etkileri ÖzetBu çalışmanın amacı, koçlarda L-Ergothionin (LE), sistein ve N-asetil sisteinin (NAC) farklı dozlarının çözüm sonu sperma parametreleri üzerine etkisini belirlemekti. Dört Tuj ırkı koçtan alınan ejakülatlar değerlendirildi ve 35°C'de karışım yapıldı. Sperma örnekleri, LE (5, 10 mM), Sistein (5, 10 mM), NAC (5, 10 mM) içeren veya antioksidan içermeyen (kontrol), yağsız süt temelli sulandırıcıyla sulandırılarak, 0.25 ml'lik payetlere dolduruldu. Payetler 5°C'ye 2 saat süreyle soğutuldu, sıvı azot buharında (yaklaşık -120°C) 15 dak. süre ile donduruldu ve çözme işlemine kadar sıvı azot içinde saklandı. Payetler su banyosunda (37°C'de 1 dak.) çözüldü. Motilite, canlılık, anormal akrozom, toplam anormalite ve membran bütünlüğü (hipoozmotik şişme testi, HOST) yüzdeleri istatistiksel olarak değerlendirildi. Ayrıca, toplam antioksidan kapasite (TAK) ve toplam oksidatif stres (TOS), replikasyonlardan elde edilen örneklerde değerlendirildi. LE'nin motilite (sistein hariç), canlılık, akrozom hasarı, total morfolojik anormalite, HOST ve TAK oranları bakımından, NAC ve sisteine üstünlük sağladığı görüldü (P<0.05). Yine de, LE ve kontrol grubu arasında istatistiksel bir fark yoktu (P>0.05). Sistein ve NAC'ın koçlarda çözüm sonu spermatolojik parametreler üzerine bazı olumsuz etkileri belirlen...

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Some effects of administering ergothioneine to rats

Cited by 26 publications

References 11 publications

Uptake and antioxidant effects of ergothioneine in human erythrocytes

Uptake and antioxidant effects of ergothioneine in human erythrocytes

The metabolism of 35S-labelled 2-thiolhistidine and ergothioneine in the rat

Koç Spermasının Dondurulması Üzerine L-Ergothionin, N-asetil sistein ve Sisteinin Etkileri

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