Some Aspects of Specimen Collection and Stability in Trace Element Analysis of Body Fluids

Anand, V. D.; White, James M.; Nino, Hipolito V.

doi:10.1093/clinchem/21.4.595

Cited by 29 publications

(3 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many metabolic disorders in man are accompanied by alterations in the concentration of one or more trace elements in some body fluids, especially blood serum or plasma. [ 11 15 ]…”

Section: Discussionmentioning

confidence: 99%

Quantitative analysis of serum levels of trace elements in patients with oral submucous fibrosis and oral squamous cell carcinoma: A randomized cross-sectional study

Mahesh

et al. 2014

J Oral Maxillofac Pathol

View full text Add to dashboard Cite

Background and Objectives:Metabolic disorders, oral precancerous conditions and oral cancer are accompanied by alterations in the concentration of one or more trace elements like copper, iron, zinc, magnesium etc., in some body fluids, especially blood serum or plasma, which can help not only in the early diagnosis and treatment but also in prognosis. The objective of the study is to evaluate the levels of circulating trace elements (copper, iron, magnesium, zinc and calcium) in serum of patients with Oral Submucous Fibrosis (OSF) and Oral Squamous Cell Carcinoma (OSCC), to analyze the alteration and identify the best predictors amongst these parameters for disease occurrence and progression and their association with areca nut and betel quid chewing habits.Materials and Method:Serum levels of trace elements (copper, iron, magnesium, zinc and calcium) were estimated using electronic absorption colorimetric method. These levels were compared with controls and statistically evaluated using ANOVA and POST-HOC TUKEY tests.Results:The data analysis revealed that serum copper levels increased gradually from precancer to cancer, as the duration of betel quid chewing habit increased. However, serum iron, magnesium, zinc levels were decreased significantly in both the groups. Serum calcium levels were increased in the cancer group owing to bone resorption in the later stages of the disease, whereas it was close to normal in OSF patients. Among all the trace elements, the best predictor for occurrence of both the lesions was copper.Conclusion:The present study shows that the above trace elements may be associated with the pathogenesis and progression of OSF and OSCC. Betel quid and areca nut chewing habits are frequently associated with both disease states and may play a role in altering the serum levels of these trace elements. Concerted efforts would, therefore, help in early detection, management and monitoring the efficacy of treatment.

show abstract

“…Many metabolic disorders in man are accompanied by alterations in the concentration of one or more trace elements in some body fluids, especially blood serum or plasma. [ 11 15 ]…”

Section: Discussionmentioning

confidence: 99%

Quantitative analysis of serum levels of trace elements in patients with oral submucous fibrosis and oral squamous cell carcinoma: A randomized cross-sectional study

Mahesh

et al. 2014

J Oral Maxillofac Pathol

View full text Add to dashboard Cite

show abstract

“…On the other hand, many studies have shown substantial agreement between reported RBC levels of copper and zinc and reported serum levels (Fredricks et al, 1960;Davies et al, 1968;Carson et al, 1986). Thus, often the optimal matrix of choice is greatly dependent on the element of interest, mechanism of interest (intracellular vs. extracellular), and available collection and storage techniques (Anand et al, 1975;Savory and Wills, 1992;Subramanian, 1995).…”

Section: Other Contributory Variablesmentioning

confidence: 99%

Trace and Non-Trace Elements in Blood Cells of Bottlenose Dolphins (Tursiops Truncatus): Variations With Values From Liver Function Indicators

Sorensen

Venn‐Watson

Ridgway

2008

Journal of Wildlife Diseases

View full text Add to dashboard Cite

Alterations in trace and non-trace element homeostasis have been associated with both normal physiologic and pathologic processes of many species. Changes in copper and zinc, for instance, have been associated with liver disease in humans and dogs. While liver disease has been documented in marine mammals, associations of liver disease with trace and non-trace elements have not been determined. The goal of this study was to assess potential elemental associations with clinically relevant changes in liver enzymes of bottlenose dolphins (Tursiops truncatus) and to compare observed associations to what has been reported in other species. Blood cell samples were collected from 37 healthy bottlenose dolphins, maintained by the Navy Marine Mammal Program (MMP), between 1991 and 1992. Twenty-one trace and non-trace elements were assessed along with a standard liver enzyme function profile, and trace element associations to specific liver enzymes were determined. In this study, of the 21 blood cell elements assessed, 19 were measured within detectable limits in at least one of the blood samples, and 10 trace elements were found to be associated with at least one of the liver function indicators. Many of these same associations have been documented in various forms of liver disease in other species, including the associations of increases in copper and decreases in zinc with both elevated alanine aminotransferase (ALT) and gamma-glutamyl transferase (GGT). The observed analogous associations between changes in blood trace and non-trace elements and liver function indicators of bottlenose dolphins and other species may indicate similar pathologic processes and functions of some elements. Given the results of this study, additional research is warranted to further elucidate associations of trace and non-trace elements to liver disease in bottlenose dolphins.

show abstract

“…Previously documented by Anand et al were some methods for specimen collection and the stability of trace element analysis. 39 They describe how the integrity of the specimen could be compromised even before it is analyzed, by contamination during collection and processing, or by attenuation of the analyte concentration during storage. They highlight the importance of controlled procedures at every stage to mitigate such errors.…”

Section: ⎛ ⎝mentioning

confidence: 99%

Review—Micro-Fuel Cell Principal Biosensors for Monitoring Transdermal Volatile Organic Compounds in Humans

et al. 2022

View full text Add to dashboard Cite

Knowledge of transduction mechanisms in biosensing applications paves the way for ultrasensitive and dynamic detection in living systems. Real-world biosensing applications where ultrasensitivity and dynamic detection are paramount include monitoring the anesthetic agent concentration during surgery; the slightest variation in concentration can potentially result in a life-threatening overdose or, on the other end of the spectrum, the patient's awareness during the procedure. We review the benefits and functions of the transcutaneous biosensor device compared with other current technology and discuss the sensor's capability to accurately measure volatile anesthetic gas concentration in blood using fuel cell technology. We review fundamental concepts of fuel-cell technology for bio-sensing applications. The fuel cell sensor can also continuously monitor other volatile organic compounds making it versatile with numerous potential applications.

show abstract

Some Aspects of Specimen Collection and Stability in Trace Element Analysis of Body Fluids

Cited by 29 publications

References 24 publications

Quantitative analysis of serum levels of trace elements in patients with oral submucous fibrosis and oral squamous cell carcinoma: A randomized cross-sectional study

Quantitative analysis of serum levels of trace elements in patients with oral submucous fibrosis and oral squamous cell carcinoma: A randomized cross-sectional study

Trace and Non-Trace Elements in Blood Cells of Bottlenose Dolphins (Tursiops Truncatus): Variations With Values From Liver Function Indicators

Review—Micro-Fuel Cell Principal Biosensors for Monitoring Transdermal Volatile Organic Compounds in Humans

Contact Info

Product

Resources

About