Some aspects of evolutionary pharmacology

Michelson, M.J.

doi:10.1016/0006-2952(74)90551-6

Cited by 21 publications

(11 citation statements)

References 9 publications

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“…That diverse expression of muscarinic receptor function is found in lower species such as molluscs, in which acetylcholine both attenuates heart rate and stimulates the central nervous system (3,4), together with the structural similarities in the receptors demonstrated here and previously (1), supports the concept that only one major structural form of the receptor may exist (1). Response diversity found with muscarinic receptors in different tissues may therefore be the result of interactions between the receptor protein and different effector molecules in the plasma membrane.…”

Section: Discussionsupporting

confidence: 55%

“…It appears as though the muscarinic receptor may be highly conserved, perhaps throughout a large part of the evolutionary process. There is evidence to suggest that atropine-sensitive acetylcholine responses, indicative of the presence of muscarinic receptors, exist throughout much of the phylogenetic tree, in molluscs, annelids, and arthropods (3,4), including insects, in which muscarinic receptor-specific radioligand binding has been reported (5-7). a1-Adrenergic receptor monomers are proteins with a molecular mass of 80,000-85,000 daltons (8)(9)(10) with a membrane topology and tryptic digest map similar to those of muscarinic receptors (9,10).…”

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confidence: 99%

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Monoclonal antibodies detect the conservation of muscarinic cholinergic receptor structure from Drosophila to human brain and detect possible structural homology with alpha 1-adrenergic receptors.

Venter¹,

Eddy²,

Hall³

et al. 1984

Proc. Natl. Acad. Sci. U.S.A.

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Muscarinic cholinergic receptors isolated from Drosophila heads, rat and human brain, dog heart, and monkey ciliary muscle were examined for structural similarities/differences by utilizing isoelectric focusing, sodium dodecyl sulfate/polyacrylamide gel electrophoresis, and monoclonal antibody crossreactivity. Muscarinic The muscarinic cholinergic receptor mediates various central nervous system activities as well as the function of the parasympathetic side of the autonomic nervous system, including heart rate attenuation and smooth muscle contraction in the eye, intestine, and airways. This laboratory has investigated the structure of the muscarinic cholinergic receptor and found it to be a monomeric protein with a molecular mass of 80,000 daltons (1). Topology studies indicate that the muscarinic receptor is similar to the nicotinic receptor (2) in that over 50% of the receptor protrudes into the extracellular aqueous space and 14% of the receptor is on the cytoplasmic side of the membrane (1).In contrast to the adrenergic receptor system, major differences do not appear to exist in the structure of muscarinic cholinergic receptors isolated from vertebrate heart, brain, and smooth muscle (1). It appears as though the muscarinic receptor may be highly conserved, perhaps throughout a large part of the evolutionary process. There is evidence to suggest that atropine-sensitive acetylcholine responses, indicative of the presence of muscarinic receptors, exist throughout much of the phylogenetic tree, in molluscs, annelids, and arthropods (3, 4), including insects, in which muscarinic receptor-specific radioligand binding has been reported (5-7). a1-Adrenergic receptor monomers are proteins with a molecular mass of 80,000-85,000 daltons (8-10) with a membrane topology and tryptic digest map similar to those of muscarinic receptors (9,10). In the present study we describe the production of monoclonal antibodies specific for muscarinic cholinergic and a1-adrenergic receptors and their use in detecting structural similarities between muscarinic cholinergic receptors isolated from a wide variety of tissues and between muscarinic receptors and rat liver a1-adrenergic receptors. were from New England Nuclear. Other materials were as described (1,(8)(9)(10)(11). MATERIALSTissues and Membranes. Rat and human brain membranes were prepared as described (1). Wild-type Drosophila melanogaster heads were separated from bodies as described (12) and stored at -80°C prior to use. Heads were homogenized in homogenization buffer, consisting of 5 mM sodium phosphate at pH 7.3, 1% sucrose, 10 mM

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Section: Discussionsupporting

confidence: 55%

mentioning

confidence: 99%

Monoclonal antibodies detect the conservation of muscarinic cholinergic receptor structure from Drosophila to human brain and detect possible structural homology with alpha 1-adrenergic receptors.

Venter¹,

Eddy²,

Hall³

et al. 1984

Proc. Natl. Acad. Sci. U.S.A.

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“…1) was the earliest to appear during evolution (MICHAELSON 1974). 1) was the earliest to appear during evolution (MICHAELSON 1974).…”

Section: Acetylcholinementioning

confidence: 95%

Pharmacology of the Skin I

Greaves¹,

Shuster²

1989

Handbook of Experimental Pharmacology

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“…The sensorium is a subject of particular interest, as the great Russian cholinergiker; Mikhail Michelson (1974) denied as late as in the 1970s that sensorium exhibits cholinergic correlates. Yet, the presence of cholinergic pathways in the retina, olivocochlear bundle, olfactory bulb, pedunculopontine projections, geniculorecipient layers, superior colliculus, and somatosensory, auditory, and visual cortices argues against Michelson's notions, and proven cholinergic correlates were established for audition, balance, vision, and nociception (see Karczmar 2007); cholinergic nociception may involve as well the spinal sites.…”

Section: Functions Eeg Phenomena Emotions and Behaviorsmentioning

confidence: 99%

Do all Human Functions and Behaviors, as well as the “Self”, Have Cholinergic Correlates?

Karczmar

2009

J Mol Neurosci

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In the course of the five most recent International Symposia on Cholinergic Mechanisms (ISCMs) as well as at this XIII ISCM, the focus was mostly on matters microscopic and molecular, except for solitary excursions into memory and learning, addiction, and clinical and toxicological subjects. In fact, this is also true for many recent reviews and meetings concerned with the cholinergic transmission. Yet, macroscopic and overt phenomena that are of great importance have strong cholinergic correlates. To remedy this lacuna, macromolecular overt cholinergic brain-dependent activities will be reviewed such as reflex automatic phenomena, EEG functions, behaviors, and the sense of the "self" or "self-awareness", particularly the difficulties associated with neuroscientific explanation of the "self," and the many approaches to this dilemma will be discussed.

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Some aspects of evolutionary pharmacology

Cited by 21 publications

References 9 publications

Monoclonal antibodies detect the conservation of muscarinic cholinergic receptor structure from Drosophila to human brain and detect possible structural homology with alpha 1-adrenergic receptors.

Monoclonal antibodies detect the conservation of muscarinic cholinergic receptor structure from Drosophila to human brain and detect possible structural homology with alpha 1-adrenergic receptors.

Pharmacology of the Skin I

Do all Human Functions and Behaviors, as well as the “Self”, Have Cholinergic Correlates?

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