Somatostatin and substance P-like immunoreactivity in the auditory brainstem of the adult rat

Wynne, Brigitte; Robertson, Donald

doi:10.1016/s0891-0618(97)00219-6

Cited by 29 publications

(18 citation statements)

References 18 publications

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“…Central nervous system areas already found to contain SPL-IR in rats were labeled in the least shrew, while areas that repeatedly appear devoid of SPL-IR in other species contained only the occasional individual immunostained fiber or two, if anything. The brain areas found to be highest in SPL-IR in the least shrew corresponded quite well with results of SP immunohistochemistry in other, better characterized species [3,8,16,21,[28][29][30]37,47,49,53,55].…”

Section: Discussionsupporting

confidence: 77%

A histologically derived stereotaxic atlas and substance P immunohistochemistry in the brain of the least shrew (Cryptotis parva) support its role as a model organism for behavioral and pharmacological research

Ray

Darmani

2007

Brain Research

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Chemotherapy is an effective treatment but difficult to tolerate due to side effects like vomiting. Studies on the etiology of chemotherapy-related emesis have implicated brainstem nuclei and the neurotransmitter Substance P, among other substrates. Since rodents do not vomit, other species have been necessary as alternative models of chemotherapy-induced emesis. Of these, the least shrew (Cryptotis parva) has proven valuable due to its small size, hardiness, and close phylogenetic relationship with primates. However, very little neuroanatomical data on C. parva exist. We used histological and immunohistochemical techniques to provide neuroanatomical data to help validate C. parva as a model organism, especially for emesis research. Brains were sectioned and stained for Nissl substance or myelin, or immunofluorescently labeled for Substance P. Sections were photographed, traced, and reconstructed with standardized zero points, and these data used to create a stereotaxic atlas. The brain of C. parva was similar to but smaller than other mammalian brains, with the cerebellum and hippocampus demonstrating the biggest differences. Differences appeared to be related to the small size of the brain and the metabolic compromises required of such a small mammal. Substance P-like immunoreactivity (SPL-IR) was semiquantitatively mapped, and correlated very well with SPL-IR observed in other species. Dense SPL-IR areas included the periaqueductal grey, trigeminal nuclei, dorsal raphe, and emesis-related brainstem nuclei including the area postrema and solitary tract nucleus. These data demonstrate that the anatomical differences between C. parva and other mammals will not preclude its use as a model organism.

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Section: Discussionsupporting

confidence: 77%

A histologically derived stereotaxic atlas and substance P immunohistochemistry in the brain of the least shrew (Cryptotis parva) support its role as a model organism for behavioral and pharmacological research

Ray

Darmani

2007

Brain Research

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“…At its ventro-caudal aspect, the large substance P-positive tract appears to curl around the ventro-lateral aspect of the LSO and apparently gives rise to varicose fibres that penetrate the VNTB (Sakanaka et al, 1982;Takatsuki et al, 1983;Wynne and Robertson, 1997). The density of fibres within the VNTB seems to vary somewhat according to the immunocytochemical technique employed, however, the findings are in agreement with the demonstration of substance P receptors in neurones of the VNTB (Maeno et al, 1993;Nakaya et al, 1994).…”

Section: Substance Psupporting

confidence: 71%

“…In the rat, a major substance P-positive fibre tract has been described coursing in the lateral lemniscus (Takatsuki et al, 1983;Wynne and Robertson, 1997). This fibre tract appears to be continuous with a positively staining curtain-like plexus lying immediately dorsal to the SOC, also described by Tallaksen-Greene et al (1993) and Sakanaka et al, (1982) in rats.…”

Section: Substance Pmentioning

confidence: 84%

“…As for many of the peptides, the densest accumulation of positive fibres has been reported in the zone immediately dorsal to the LSO and SPN, with varying degrees of penetration of the SOC itself depending on the particular subdivision and species. In the adult rat and guinea pig, the densest innervation has been reported for the MNTB and VNTB, with some minor innervation of the medial zones of the LSO (Johansson et al, 1984;Krisch, 1981;TaberPierce et al, 1985;Takatsuki et al, 1981;Wynne and Robertson, 1997). Morley (1985) and Wynne and Robertson (1997) show striking clusters of somatostatinpositive nerve endings in close apposition to principal cells of the MNTB (see also Fig.…”

Section: Somatostatinmentioning

confidence: 92%

“…The inferior colliculus and lateral lemniscal nuclei could also conceivably be a source of the somatostatinpositive innervation of the SOC since mRNA for somatostatin, and immunoreactivity for the peptide, are present throughout these nuclei (Sekitani et al, 1990;Taber-Pierce et al, 1985;Takatsuki et al, 1981;Wynne et al, 1995;Wynne and Robertson, 1997). However, although the inferior colliculus is a possible source of the somatostatin innervation of the VNTB and other areas of the SOC, it seems unlikely that the striking somatostatin-positive endings in the MNTB could arise from the inferior colliculus, since anterograde labelling studies have failed to reveal any direct innervation of the MNTB by inferior colliculus neurones.…”

Section: Origin Of Peptidergic Innervationmentioning

confidence: 99%

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