Somatostatin 28: Comparison with somatostatin 14 for plasma kinetics and low-dose effects on the exocrine pancreas in dogs

Vaysse, Nicole; Chayvialle, Jean‐Alain; Pradayrol, Lucien; Estève, Jean-Pierre; Susini, C.; Lapuelle, J.; Descos, F.; Ribet, A

doi:10.1016/0016-5085(81)90494-7

Cited by 47 publications

(17 citation statements)

References 14 publications

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“…The infused S-14 disappeared with a half-life of 1 06+ 019 min; similar results have been reported by others working without plasma extraction in dogs (Schusdziarra, Harris, & Unger, 1979;Vaysse et al 1981) and man (Sheppard, Shapiro, Pimstone et al 1979) and with extraction in pigs (Hilsted & Hoist, 1982) and man (Bethge, Diel, Rosick & Holz, 1981). The half-life for S-28 (2-14+ 0-54min) was remarkably close to that obtained in dogs (Vaysse et al 1981;Polonsky et al 1982;Seal et al 1982) but considerably longer than that obtained by Patel & Wheatley (1983) in rats (1-35 min). This discrepancy may result from a true species difference in the clearance of S-28, or from the assay procedure as well as the use of the constant infusion technique in man, dogs and ducks, compared with a single injection method in rats (Patel &Wheatley, 1983).…”

Section: Discussionsupporting

confidence: 89%

“…(S-28), a naturally occurring TVterminal extended form of the tetradecapeptide S-14 isolated from porcine intestine (Pradayrol, Jornvall, Mutt & Ribet, 1980), is more potent than the tetra¬ decapeptide for inhibition of pituitary secretion (Susini, Esteve & Vaysse, 1980;Brazeau, Ling, Esch et al 1981) and insulin and glucagon release (Tannenbaum, Ung & Brazeau, 1982). These effects could be due to a slower catabolism of this form in plasma (Vaysse, Chayvialle, Pradayrol et al 1981;Seal, Yamada, Debas et al 1982;Patel & Wheatley, 1983) and/or at the tissue level.…”

Section: Introductionmentioning

confidence: 98%

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Characterization of somatostatin in peripheral and portal plasma in the duck: in-vivo metabolism of somatostatin-28 and-14

Guenot¹,

Strosser²,

Mialhe³

1984

Journal of Endocrinology

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A sensitive and specific radioimmunoassay without an extraction step was developed for somatostatin in duck plasma. Degradation of Tyr1-125I-labelled somatostatin-14 (S-14) averaged 2% for blood collected with EDTA and zymofren. Recovery of somatostatin-like immunoreactivity (SLI), added to the plasma, averaged 91% for S-14 and 86% for S-28. Chromatographic analysis of portal plasma on Sephadex G-25 showed three peaks: one peak coeluted with cytochrome c (mol. wt 12500) in the void volume and was called 'big' somatostatin; of the two smaller forms, one coeluted with synthetic S-28 and the other with synthetic S-14; these were immunologically and physicochemically indistinguishable from synthetic S-28 and S-14. In peripheral plasma only the large form of somatostatin, 'big' somatostatin, was found. The mean portal plasma concentration of SLI was 4.1 +/- 0.41 microgram/l (n = 11, range 2.8-5.1 micrograms/l). In peripheral plasma the SLI concentration was 1.05 +/- 0.45 microgram/l (n = 11, range 0.84-1.2 microgram/l). The metabolic clearance rate, distribution volume and calculated half-life values were 63.1 +/- 14 ml/kg per min, 40.9 +/- 8.9 ml/kg and 1.06 +/- 0.19 min for S-14 compared with 45.7 +/- 7 ml/kg per min, 14.8 +/- 2.5 ml/kg and 2.14 +/- 0.54 min for S-28. These results indicated that S-28 was cleared from plasma at a slower rate than S-14 in the duck. It was concluded that: (1) portal plasma SLI was four times higher than peripheral SLI; (2) SLI in portal plasma existed as 'big' somatostatin, S-28 and S-14, whereas in peripheral plasma it existed mainly as 'big' somatostatin; (3) in-vivo studies indicated that S-28 was metabolized less rapidly than S-14.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 98%

Characterization of somatostatin in peripheral and portal plasma in the duck: in-vivo metabolism of somatostatin-28 and-14

Guenot¹,

Strosser²,

Mialhe³

1984

Journal of Endocrinology

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“…In contrast, in dogs (Vaysse et al 1981a;Konturek et al 1981) the peptides are reported to have a similar effect in suppressing insulin secretion. The greater activity of SS-28 may be due to the higher steady state levels achieved after continuous infusion as demonstrated by the present study and in dogs (Vaysse et al 1981b). However, SS-28 may also have a greater intrinsic affinity for binding the human ß cell somatostatin receptor as has been shown in rat insulinoma cells (Brown et al 1981).…”

Section: Discussionmentioning

confidence: 63%

Inhibition of pancreatic hormone secretion by somatostatin-28 and somatostatin-14 in man

Klaff¹,

Barron²,

Levitt³

et al. 1983

Acta Endocrinologica

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“…All tests were performed after an overnight fast and after withdrawal of any antisecretory medication for at least 5 days before the study. In tests with cephalic-phase stimulation, a double-lumen Dreiling tube was passed under fluoroscopic control for gastric juice collection as described previously (12). Residual gastric contents were discarded, and the gastric aspirates were collected with a suction pump in 15-min periods.…”

Section: Methodsmentioning

confidence: 99%

“…It showed that SS-28 mimics most of the gastrointestinal secretory effects of SS-14 (10) but results in higher immunoreactive somatostatin plasma levels, probably due to the slower meta-bolic rate of SS-28 in plasma and in other tissues In this study, performed in healthy subjects, we compared both the effects of cephalic-and gastrointestinal-phase stimulation on somatostatin-like immunoreactivity (SLI) plasma levels and the actions of exogenous SS-14 and SS-28 on gastric secretion and plasma hormonal responses to these stimulations. (11,12).…”

mentioning

confidence: 98%

Effects of Somatostatin-14 and Somatostatin-28 on Plasma Hormonal and Gastric Secretory Responses to Cephalic and Gastrointestinal Stimulation in Man

Konturek

Kwiecie

Obtuowicz

et al. 1985

Scandinavian Journal of Gastroenterology

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This study was designed to determine the influence of cephalic and gastrointestinal meal stimulation on plasma levels of somatostatin-like immunoreactivity (SLI) and to compare plasma hormonal and gastric secretory effects of somatostatin-14 (SS-14) and its putative prohormone, somatostatin-28 (SS-28), in humans. Cephalic stimulation induced by modified sham feeding did not affect plasma SLI, whereas a gastric liver extract meal caused a significant increase in SLI. Infusion of SS-28 dose-dependently suppressed gastric acid, serum gastrin, and plasma pancreatic polypeptide (PP) responses to cephalic and gastrointestinal stimulation. SS-28 was equipotent with SS-14 as gastric inhibitor when compared on the basis of molar doses infused but was 4-10 times less potent on the basis of plasma SLI concentrations obtained. A lower and more physiological dose of SS-14 (75 pmol/kg-h) reduced gastric acid and PP responses but failed to affect the serum gastrin response to a meal; whereas a larger, pharmacological dose (500 pmol/kg-h) also suppressed serum gastrin responses. We conclude that meal releases SLI into the circulation and that SS-28 mimics the gastric secretory and plasma hormonal effects of SS-14 but is several times less potent than SS-14 in terms of circulating hormone levels.

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Somatostatin 28: Comparison with somatostatin 14 for plasma kinetics and low-dose effects on the exocrine pancreas in dogs

Cited by 47 publications

References 14 publications

Characterization of somatostatin in peripheral and portal plasma in the duck: in-vivo metabolism of somatostatin-28 and-14

Characterization of somatostatin in peripheral and portal plasma in the duck: in-vivo metabolism of somatostatin-28 and-14

Inhibition of pancreatic hormone secretion by somatostatin-28 and somatostatin-14 in man

Effects of Somatostatin-14 and Somatostatin-28 on Plasma Hormonal and Gastric Secretory Responses to Cephalic and Gastrointestinal Stimulation in Man

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