Somatic Hybridization of Eggplant (Solanum melongena L.) with Its Close and Wild Relatives

Sihachakr, D.; Daunay, Marie‐Christine; Serraf, I.; Chaput, M. H.; Mussio, Isabelle; Haïcour, R.; Rossignol, L.; Ducreux, G.

doi:10.1007/978-3-642-57945-5_17

Cited by 35 publications

(18 citation statements)

References 34 publications

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“…In vegetable crops of the Solanaceae, resistance to soil-borne diseases has been introduced from wild into cultivated species through somatic fusion; this is particularly true in tomato (Lefrancois et al 1993) and potato (Austin et al 1988;Deimling et al 1988;Brown et al 1995;Laferriere et al 1999;Fock et al 2000). Likewise, somatic hybridisation has been successfully applied to obtain interspecific hybrids between eggplant (Solanum melongena) and its relatives resistant to soil-borne diseases caused by nematodes, Verticillium, Fusarium and Ralstonia (Gleddie et al 1986; Guri and Sink 1988;Sihachakr et al 1988;Daunay et al 1993;Sihachakr et al 1994;Jarl et al 1999;Collonnier at al. 2001).…”

Section: Introductionmentioning

confidence: 97%

Androgenic dihaploids from somatic hybrids between Solanum melongena and S. aethiopicum group gilo as a source of resistance to Fusarium oxysporum f. sp. melongenae

et al. 2002

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Dihaploid plants were obtained through anther culture of somatic hybrids between eggplant and Solanum aethiopicum gilo. The androgenic origin of the dihaploids was demonstrated by ploidy determination (flow cytometry and chloroplast counting) and isozyme and molecular (I-SSR and RAPDs) analyses. The androgenic plants showed significant morphological variability in the traits analysed. Pollen viability in the diploid androgenic plants was drastically reduced with respect to the somatic hybrids; however most of the dihaploids produced parthenocarpic fruits. S. aethiopicum and the somatic hybrids showed complete resistance to fungal wilt caused by Fusarium oxysporum f. sp. melongenae. Out of the 41 dihaploids inoculated, 34 were symptomless. The population of androgenic plants developed may represent a useful source for introgression of the Fusarium resistance trait into eggplant.

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Section: Introductionmentioning

confidence: 97%

Androgenic dihaploids from somatic hybrids between Solanum melongena and S. aethiopicum group gilo as a source of resistance to Fusarium oxysporum f. sp. melongenae

et al. 2002

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“…1B). Sihachakr et al (1994) reported that calli that were probably somatic hybrids between S. melongena and S. aethiopicum could be identified because of their ability to grow faster and to regenerate earlier than calli of the parent species. In experiments using the same species, Collonnier et al (2001) selected fast growing calli and, after regeneration, confirmed that 18-28% of these calli were in fact hybrids.…”

Section: Selection Of Somatic Hybrids and Their Characterizationmentioning

confidence: 98%

A somatic hybrid between Solanum integrifolium and Solanum violaceum that is resistant to bacterial wilt caused by Ralstonia solanacearum

Tamura¹,

Murata²,

Mukaihara³

2002

Plant Cell Reports

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Protoplast fusion was performed between a commercial rootstock variety of Solanum integrifolium that is widely used in the cultivation of edible eggplant, and Solanum violaceum, a wild eggplant species tolerant to bacterial wilt. One of the somatic hybrid lines (27-14) obtained showed morphological traits intermediate between those of its parents. Its hybrid nature was confirmed by analysis of random amplified polymorphic DNA and restriction fragment length polymorphism patterns. Microscopic observation revealed that the line was amphidiploid. The hybrid line and both parent species were infected with a virulent strain of Ralstonia solanacearum by the root cutting method. Line 27-14 developed much less severe symptoms of bacterial wilt than the susceptible S. integrifolium. Twenty days after inoculation, the bacterial density in the shoot apex tissue was significantly lower in the hybrid than in S. integrifolium, suggesting that the multiplication of bacteria was inhibited in line 27-14.

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“…Numerous somatic hybrid plants have been obtained in dicots solanaceae like potato (Debnath and Wenzel, 1987), eggplant (Sihachakr et al, 1994) and in citrus (Crosser et al, 2000). Conversely, within the monocots like poaceae (cereals) and musaceae (banana), somatic hybridisation has proven to be very difficult.…”

Section: Introductionmentioning

confidence: 98%

Protoplast Fusion in Banana (Musa spp.): Comparison of Chemical (PEG: Polyethylene Glycol) and Electrical Procedure

Assani

Chabane²,

Haïcour

et al. 2005

Plant Cell Tiss Organ Cult

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Optimization of protoplast fusion parameters is a prerequisite for the establishment of somatic fusion technology for banana breeding. In the present investigations, we compared the most frequently used fusion methods: the electrofusion technique and chemical procedure (polyethylene glycol). With regard to frequency of binary fusion, protoplast fusion with the fusogen polyethylene glycol was best. Conversely, electric fusion was found to be better with respect to mitotic activities, somatic embryogenesis and plantlet regeneration rate.

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Somatic Hybridization of Eggplant (Solanum melongena L.) with Its Close and Wild Relatives

Cited by 35 publications

References 34 publications

Androgenic dihaploids from somatic hybrids between Solanum melongena and S. aethiopicum group gilo as a source of resistance to Fusarium oxysporum f. sp. melongenae

Androgenic dihaploids from somatic hybrids between Solanum melongena and S. aethiopicum group gilo as a source of resistance to Fusarium oxysporum f. sp. melongenae

A somatic hybrid between Solanum integrifolium and Solanum violaceum that is resistant to bacterial wilt caused by Ralstonia solanacearum

Protoplast Fusion in Banana (Musa spp.): Comparison of Chemical (PEG: Polyethylene Glycol) and Electrical Procedure

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