Somatic hybridization in potato: use of ?-irradiated protoplasts of Solanum pinnatisectum in genetic reconstruction

Sidorov, V. A.; Zubko, Mikhajlo K.; Kuchko, A. A.; Komarnitsky, I. K.; Gleba, Yu. Yu.

doi:10.1007/bf00274719

Cited by 80 publications

(20 citation statements)

References 19 publications

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“…Potato was also found to be amenable to these methods. Anther-derived haploid production (Wenzel and Uhrig 1981), protoplast fusion (Skarzhinskaya et al 1982), somatic hybridization (Sidorov et al 1987), genetic transformation ) and other in vitro techniques have been widely used on potato for breeding and research purposes (reviewed in Bradshaw andMackay 1994, Vreugdenhil et al 2007).…”

Section: Potato Biotechnologymentioning

confidence: 99%

Plant biotechnology for deeper understanding, wider use and further development of agricultural and horticultural crops

Elomaa¹,

Joensuu²,

Korpelainen³

et al. 2008

AFSci

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Plants bind solar energy to organic matter via photosynthesis and assimilation of carbon dioxide from the atmosphere and comprise the major source of nutrition and bioenergy. Plant biotechnology contributes to solution of important constraints in food and feed production and creates new technologies and applications for the sustainable use of plant resources. Genome-wide approaches such as massive parallel sequencing and microarrays to study gene expression, molecular markers for selection of important traits in breeding, characterization of genetic diversity with the aforementioned approaches, and somatic hybridization and genetic transformation are important tools in plant biotechnology. In this paper, studies carried out on enhanced resistance to viruses and tolerance of cold stress in potato, genetic modification of flower pigmentation and morphology in gerbera, production of edible vaccines in transgenic barley seeds, and expression of heterologous proteins for pharmaceutical purposes from vector viruses were chosen to exemplify the general utility of biotechnological approaches and also how plant biotechnology research has developed on cultivated plants at University of Helsinki. The studies reveal cellular and genetic mechanisms and provide scientific information that can be used for widening the uses of crop plants. They can also be used to detect any putative risks associated with the use of the biotechnological application in agriculture and horticulture and to develop practises which reduce any inadvertent negative consequences that plant production may have to the environment.

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Section: Potato Biotechnologymentioning

confidence: 99%

Plant biotechnology for deeper understanding, wider use and further development of agricultural and horticultural crops

Elomaa¹,

Joensuu²,

Korpelainen³

et al. 2008

AFSci

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“…По истечении этого времени в пробирку аккуратно (по стенке) добавляется 0,5 мл буфер-ного раствора с высоким рН, состоящего из компонентов А и Б. (Рас- В описываемых здесь экспериментах продукты слияния культиви-ровали на среде SW [5].…”

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Induction of chlorophyll deficient tobacco mutants as markers for cell engineering

Zubko¹,

Zubko²,

Kapranov³

1991

Biopolym. Cell

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“…Occasionally, large or small purple spots were observed on the lower surface of leaflets. Anthocyanin synthesis is a dominant trait and is expressed in true nuclear hybrids (Sidorov et al 1987). Therefore, the anthocyanin pigmentation in regenerants could serve as a visible selective marker of their hybrid nature.…”

Section: Morphological Characterizationmentioning

confidence: 99%

“…Backcross progenies of somatic hybrids have been developed (Helgeson et al 1998;Gavrilenko et al 2003). Fusion with an irradiated donor (Sidorov et al 1987) or microprotoplasts (Ramulu et al 1995) led to partial transfer of the genome, limiting the risk of introducing unwanted characteristics from wild species into the breeding program.…”

mentioning

confidence: 99%

Somatic hybridization between Solanum tuberosum and S. cardiophyllum

Shi¹,

Chen²,

Li³

et al. 2006

Can. J. Plant Sci.

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. 2006. Somatic hybridization between Solanum tuberosum and S. cardiophyllum. Can. J. Plant Sci. 86: 539-545. The wild diploid Mexican species, Solanum cardiophyllum Lindl. (2n = 2x = 24), is resistant to important potato diseases. However, introgression of resistance to the tetraploid cultivated potato (S. tuberosum L.) (2n = 4x = 48) by conventional crossing is not feasible due to the difference in their endosperm balance number between these species. Somatic hybrids between S. cardiophyllum and S. tuberosum were produced for the first time by electrofusion of protoplasts isolated from young leaves of each parental line. The hybrid nature of the regenerated plants was confirmed based on morphology, chromosome number and DNA species-specific RAPD markers. All the somatic hybrids produced a violet pigmentation on their stems and petioles, which resembled the wild partner. Most of the hybrid plants had 2n = 72 chromosomes and exhibited a morphology intermediate between the two fusion parents, but with a tendency towards cultivated potato. These plants flowered and set fruit when backcrossed with their S. tuberosum fusion parent. Mots clés: Pomme de terre (Solanum tuberosum), S. cardiophyllum, fusion des protoplastes, marqueur RAPD Potatoes (Solanum tuberosum L.) are plagued by many kinds of diseases. Of these diseases, late blight, caused by Phytophthora infestans (Mont.) de Bary, is the most devastating one worldwide. Under cool wet conditions, the pathogen rapidly attacks the leaves, stems and tubers, causing significant reductions in yield and quality. All of the current commercial potato cultivars are highly susceptible to late blight. Surveys have shown that the disease costs potato growers $3 billion annually worldwide . Control of potato late blight is currently achieved through the extensive use of fungicides. Therefore, it is not surprising that seeking a genetic resource with resistant traits to the disease has long attracted potato breeders' attention. For example, the Mexican wild species Solanum demissum Lindl. was reported to be resistant to late blight (Reddick 1930). Unfortunately, the resistance was only effective against certain races of the pathogen (Black and Gallegly 1957). An accession of another Mexican wild species, S. cardiophyllum Lindl., was recently found to possess a high level of resistance to late blight . However, S. cardiophyllum and the cultivated potato, S. tuberosum are reproductively incompatible due to differences in their endosperm balance numbers although they can be crossed with several other diploid Mexican wild species (Chen et al. 2004). Thus, this prevents the exploitation of this valuable germplasm using conventional breeding methods.Protoplast fusion offers an alternative to conventional crossing as it provides a means of circumventing the sexual reproduction barriers and allows the incorporation of desirable traits from wild species into cultivated potatoes. Using somatic hybridization, several resistance genes have been introduced into cultivated potato from wild s...

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Somatic hybridization in potato: use of ?-irradiated protoplasts of Solanum pinnatisectum in genetic reconstruction

Cited by 80 publications

References 19 publications

Plant biotechnology for deeper understanding, wider use and further development of agricultural and horticultural crops

Plant biotechnology for deeper understanding, wider use and further development of agricultural and horticultural crops

Induction of chlorophyll deficient tobacco mutants as markers for cell engineering

Somatic hybridization between Solanum tuberosum and S. cardiophyllum

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