Induction of chlorophyll deficient tobacco mutants as markers for cell engineering

Self-fertile cybrids Nicotiana tabacum (+Hyoscyamus aureus) with a nucleo-plastome incompatibility

“…aureus) plants manifested the rDNA pattern of tobacco. These data are in agreement with the previous analysis of nuclear-encoded isozymes (Zubko 1992) showing the cybrid nature of the plants analysed. Partial chlorophyll-deficiency and self-fertility of the line 6R20-15…”

“…Twelve lines of plants were recovered with a relatively normal morphology characteristic for tobacco. They all exhibited identical patterns of amylase and aspartate-aminotranspherase isozymes of the tobacco type (Zubko 1992). The plants were able to grow in the soil.…”

“…The albino plastome mutant of N. tabacum L., line R100a1 (Zubko et al 1991) was used as a recipient. Aseptic plants of both species were propagated on basal Murashige and Skoog medium with 30 g/l of sucrose at a temperature of 25°C and illumination regime 10/14 h of darkness/light.…”

“…Protoplasts of albino plastome tobacco mutant R100a1 never recovered green plants in our previous experiments (Zubko et al 1991). Since protoplasts of H. aureus were inactivated in their divisions by γ-irradiation, all green clones derived after fusing protoplasts of these two partners were supposed to be either asymmetric hybrids or cybrids always containing a plastid genome of H. aureus.…”

“…The plants were able to grow in the soil. Most of them were classified as asymmetric nuclear hybrids on the basis of irregular leaf/flower morphologies, sterility and the presence of characteristic H. aureus bands in their esterase isozyme patterns (Zubko 1992). Three lines, 6R20-06, 6R20-15 and 6R20-32, with the most regular morphology and tobacco isozyme patterns, were further analysed as putative cybrids.…”