Somatic Embryogenesis in Grapevine

Martinelli, L.; Gribaudo, Ivana

doi:10.1007/978-94-017-2308-4_13

Cited by 41 publications

(19 citation statements)

References 71 publications

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“…Success varies with virus, grape cultivar and specific approach. Somatic embryogenesis, usually adopted to regenerate plantlets in biotechnological breeding programmes (Martinelli and Gribaudo, 2001), can efficiently eliminate several grapevine viruses (Goussard et al, 1991;Schaefers et al, 1994). Similar results were obtained in Citrus (D'Onghia et al, 2001) and in sugarcane (Parmessur et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…We have investigated the presence of GLRaV-1, GLRaV-3, GVA and GRSPaV at the various stages of grapevine somatic embryogenesis: flower explants (anthers and ovaries) which are the most valuable source for somatic embryogenesis in the genus Vitis (Martinelli and Gribaudo, 2001), embryogenic and non-embryogenic calli, single somatic embryos and plants regenerated from embryogenic cultures. We report the successful elimination of GLRaV-1, GLRaV-3, GVA and GRSPaV through somatic embryogenesis from Mu¨ller-Thurgau, Grignolino and Bosco, three grapevine wine cultivars.…”

Section: Introductionmentioning

confidence: 99%

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Detection and Elimination of Viruses in Callus, Somatic Embryos and Regenerated Plantlets of Grapevine

Gambino¹,

Bondaz²,

Gribaudo³

2006

Eur J Plant Pathol

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The distribution of some grapevine viruses in flower explants, embryogenic and non-embryogenic calli, single somatic embryos and plants regenerated from embryogenic cultures was investigated by RT-PCR and ELISA. Immature anthers and ovaries of the cultivars Grignolino infected by GRSPaV, GLRaV-1 and GVA, Mu¨ller-Thurgau infected by GRSPaV and GLRaV-3 and Bosco infected by GRSPaV were cultivated on media inducing indirect somatic embryogenesis. Viruses were detected both in anthers and ovaries. Four months after culture initiation 65.6% of tested calli were infected by at least one virus; high percentages of virus infection were found in calli originating from ovaries. No virus was detected in calli tested 8 months after culture initiation, as well as in single somatic embryos or in embryo-derived plantlets. Somatic embryogenesis confirmed its effectiveness in eliminating phloem-limited grapevine viruses. Regeneration of RT-PCR negative plantlets occurred even when at least a sector of the callus was still infected: the mechanism whereby somatic embryos are freed of some viruses could be related to the rapid proliferation of embryogenic cells within the callus or to the origin of the embryogenic callus from virusfree cells within the original explant.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detection and Elimination of Viruses in Callus, Somatic Embryos and Regenerated Plantlets of Grapevine

Gambino¹,

Bondaz²,

Gribaudo³

2006

Eur J Plant Pathol

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“…According to [5], some of the advantages somatic embryogenesis has over direct in vitro propagation systems include the former's high multiplication rates, possibility of cryopreservation of embryogenic callus, the potential for scale-up in liquid suspension cultures, amenability to the use of bioreactors and the application of somatic synthetic seed technologies. In addition, somatic embryogenesis can be a very useful tool in genetics and breeding research [6]. Plantlets derived from this technique can be used to produce genetic lines that are free of viruses [7] and to introduce genes by genetic transformation [8].…”

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confidence: 99%

<i>In Vitro</i> Regeneration of <i>Curcuma caesia</i> Plantlets from Basal Part and via Somatic Embryogenesis

Zuraida¹,

Izzati²,

Nazreena³

et al. 2014

ABB

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“…Previous results in rootstocks, non viniferaspecies and scions showed that the capacity to induce somatic embryogenesis markedly varied among genotypes (Gray 1995;Kikkert et al 2001;Martinelli and Gribaudo 2001). The regeneration of transgenic grapevines has been obtained mainly from embryogenic cultures (Kikkert et al 2001), where immature floral tissues (anthers, ovules and ovaries) but also other explants (leaf, shoot tip or zygotic embryos) seem to be the more reactive explants (Gray 1995).…”

Section: Introductionmentioning

confidence: 95%

“…The regeneration of transgenic grapevines has been obtained mainly from embryogenic cultures (Kikkert et al 2001), where immature floral tissues (anthers, ovules and ovaries) but also other explants (leaf, shoot tip or zygotic embryos) seem to be the more reactive explants (Gray 1995). The regeneration competence is dependent on a complex interaction between genotype, explant source and culture media (Kikkert et al 2001;Martinelli and Gribaudo 2001). However additional research showed that genotypic differential responses could be significantly reduced (Perrin et al 2001).…”

Section: Introductionmentioning

confidence: 98%

Establishment of embryogenic cultures and plant regeneration in the Portuguese cultivar ‘Touriga Nacional’ of Vitis vinifera L.

Pinto-Sintra

2007

Plant Cell Tiss Organ Cult

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Touriga Nacional' is the most important Portuguese grapevine cultivar used for Port wine, table wine and varietal wine production. In order to obtain a reproducible plant regeneration system that allows the application of biotechnological tools to grapevine breeding, embryogenic cultures were induced from immature flowers of three Touriga Nacional selected clones. Gynoecia and anthers were cultured on Nitsch and Nitsch (Science 163:85-87, 1969) basal medium supplemented with four combinations of the growth regulators 6-benzylaminopurine (BAP), 2,4-dichlorophenoxyacetic acid (2,4-D) and indole-3-acetyl-L-aspartic acid (IASP), at 28°C, in the dark. Primary callus, observed on anthers and gynoecia in all media, produced embryogenic callus when cultured on differentiation medium, at 24°C under light. The efficiency on induction of embryogenic callus ranged from 1.2 ± 4.7% to 7.9 ± 13.8% in anthers, and from 17.9 ± 24.9% to 25.3 ± 22.9% in gynoecia. Seven lines of embryogenic cultures were established from the three clones. Multiplication of embryogenic calluses was successfully obtained in maintenance medium, at 26°C, in the dark. These embryogenic calluses produced somatic embryos when subcultured on differentiation medium, under a 16 h photoperiod. Somatic embryos were isolated and cultured on germination medium to achieve conversion which ranged from 35.3 ± 48.5% to 72.7 ± 45.6%. The plantlets obtained were cultured in medium without growth regulators. Secondary embryogenesis was also frequently observed in the hypocotyl-root transition region of somatic embryos. Although some morphological variation occurred between somatic embryos, the regenerated plantlets had a normal phenotype. Maintenance of embryogenic cultures has been achieved since 2002.

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Somatic Embryogenesis in Grapevine

Cited by 41 publications

References 71 publications

Detection and Elimination of Viruses in Callus, Somatic Embryos and Regenerated Plantlets of Grapevine

Detection and Elimination of Viruses in Callus, Somatic Embryos and Regenerated Plantlets of Grapevine

<i>In Vitro</i> Regeneration of <i>Curcuma caesia</i> Plantlets from Basal Part and via Somatic Embryogenesis

Establishment of embryogenic cultures and plant regeneration in the Portuguese cultivar ‘Touriga Nacional’ of Vitis vinifera L.

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Somatic Embryogenesis in Grapevine

Cited by 41 publications

References 71 publications

Detection and Elimination of Viruses in Callus, Somatic Embryos and Regenerated Plantlets of Grapevine

Detection and Elimination of Viruses in Callus, Somatic Embryos and Regenerated Plantlets of Grapevine

&lt;i&gt;In Vitro&lt;/i&gt; Regeneration of &lt;i&gt;Curcuma caesia&lt;/i&gt; Plantlets from Basal Part and via Somatic Embryogenesis

Establishment of embryogenic cultures and plant regeneration in the Portuguese cultivar ‘Touriga Nacional’ of Vitis vinifera L.

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<i>In Vitro</i> Regeneration of <i>Curcuma caesia</i> Plantlets from Basal Part and via Somatic Embryogenesis