Somatic Embryogenesis in Big-leaf Mahogany (Swietenia macrophylla King)

Maruyama, E.; Ishii, Keisuke

doi:10.1007/978-94-011-4774-3_3

Cited by 12 publications

(5 citation statements)

References 19 publications

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“…In timber species, somatic embryogenesis (SE) offers unique advantages, such as higher rates of propagule production per single induction event (Pullman and Johnson 2002;Yildirim et al 2006), cryopreservation of embryogenic calluses (Park 2002), rejuvenation (von Aderkas andBonga 2000;Ravindra and Nataraja 2007), the ability to produce artificial seeds (Aquea et al 2008) and, remarkably, a low rate of production of chimeric plants derived from genetic transformation experiments (Bajaj 1995;Á lvarez and Ordás 2007). Among tropical wood species, SE has been reported for other Meliacea members, such as Melia azedarach (Vila et al 2007), Azadirachta indica (Shrikhande et al 1993;Rout 2005), Swietenia macrophylla (Maruyama and Ishii 1999), and Cedrela fissilis (Vila et al 2009). …”

Section: Introductionmentioning

confidence: 95%

Induction of somatic embryogenesis and plant regeneration in the tropical timber tree Spanish red cedar [Cedrela odorata L. (Meliaceae)]

Peña-Ramírez

García-Sheseña

Hernández-Espinoza

et al. 2010

Plant Cell Tiss Organ Cult

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Spanish red cedar (Cedrela odorata L.) is a tropical timber tree native to the Americas from southern Mexico to northern Argentina. Commercial plantations are scarce and, consequently, natural populations are overexploited. Traditional propagation practices for the establishment of large-scale plantations have had limited success in this species due to the relative scarcity of seeds, its broad genetic diversity and the lack of domesticated varieties. In vitro clonal propagation provides an effective method to overcome this situation and increase the yield of commercial plantations through the rapid multiplication of elite materials. Somatic embryogenesis (SE) is one of the most promising strategies for tree propagation due to the possibility of producing artificial seeds, the ability to store and rapidly mobilize germplasm and the opportunity for genetic manipulation. We report here the induction of indirect SE in C. odorata from calli derived from immature zygotic embryos after 12 weeks of culture. Macroscopic, histological, and scanning electron microscopic analyses of the calli revealed the presence of embryogenic cell clusters that formed cotyledonary embryos with clear bipolar structures and no vascular connections with the mother tissue. Different media preparations containing combinations of diverse auxins and cytokinins are known to have different effects on the type and frequency of embryogenic structures. Embryo conversion was achieved using an MS-based medium [Murashige T, Skoog F (1962) Physiol Plant 15:473-497, 1962 supplemented with abscisic acid, and transfer to soil was successful at a rate of 75%. The method described here provides a basis for optimizing the clonal propagation and genetic manipulation of this valuable species.

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Section: Introductionmentioning

confidence: 95%

Induction of somatic embryogenesis and plant regeneration in the tropical timber tree Spanish red cedar [Cedrela odorata L. (Meliaceae)]

Peña-Ramírez

García-Sheseña

Hernández-Espinoza

et al. 2010

Plant Cell Tiss Organ Cult

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“…In conifers, the combined treatment of ABA and PEG has become the most commonly used method to induce somatic embryogenesis [ 23 ]. PEG can promote the development of ESMs by simulating drought conditions and preventing the formation of aberrant embryos [ 24 ], and 15% PEG effectively improves the rate of maturation and quality of somatic embryos in Swietenia mahagoni [ 31 ]. ABA promotes the maturation of somatic embryos and inhibits their premature germination [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

H2O2 Significantly Affects Larix kaempferi × Larix olgensis Somatic Embryogenesis

Zhu,

Zhang,

Xiong

et al. 2024

IJMS

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Larch is widely distributed throughout the world and is an important species for timber supply and the extraction of industrial raw materials. In recent years, the hybrid breeding of Larix kaempferi and Larix olgensis has shown obvious heterosis in quick-growth, stress resistance and wood properties. However, its growth and development cycle is too long to meet general production needs. In order to shorten the breeding cycle, we have for the first time successfully established and optimized a somatic embryogenesis system for Larix kaempferi × Larix olgensis. We found that the highest rate of embryonal-suspensor mass (ESM) induction was observed when late cotyledonary embryos were used as explants. The induced ESMs were subjected to stable proliferation, after which abscisic acid (ABA) and polyethylene glycol (PEG) were added to successfully induce somatic embryos. Treatment with PEG and ABA was of great importance to somatic embryo formation and complemented each other’s effect. ABA assisted embryo growth, whereas PEG facilitated the formation of proembryo-like structures. On top of this, we studied in more detail the relationship between redox homeostasis and the efficiency of somatic embryogenesis (frequency of ESM induction). During subculture, we observed the gradual formation of three distinct types of ESM. The Type I ESM is readily able to form somatic embryos. In contrast to type I, the type III ESM suffers from severe browning, contains a higher level of hydrogen peroxide (H2O2) and demonstrates a decreased ability to form somatic embryos. External treatment with H2O2 decreased the somatic embryogenesis efficiency of Type I and type III ESMs, or the higher the exogenous H2O2 content, the lower the resulting somatic embryogenesis efficiency. We found that treatment with the H2O2 scavenger DMTU (dimethylthiourea) could significantly increase the somatic embryogenesis efficiency of the type III ESM, as a result of a decline in endogenous H2O2 content. Overall, these findings have contributed to setting up a successful somatic embryogenesis system for larch production.

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“…Shortly thereafter, Maruyama et al (1989) reported organogenesis in C. odorata, also using shoot tips as the explant material, and subsequently Nunes et al (2007) have provided a set of organogenesis protocols for the closely related species, Cedrela fissilis. Successful somatic embryogenesis (SE) was initially described in the Swietenioideae, for S. macrophylla (Maruyama and Ishii 1999;Maruyama 2006), using in vitro-grown shoot tips taken from organogenic cultures. More recently, lowfrequency SE has been reported by Muñoz Tuesta (2003) from different parts of aseptically germinated C. odorata plantlets and at higher rates by Gonzalez-Rodriguez and Peña-Ramirez (2007), especially from root explants.…”

Section: Introductionmentioning

confidence: 99%

Plant regeneration in Spanish cedar, Cedrela odorata L., using zygotic embryo explants from mature seed and improvement of embryogenic nodule initiation by heat shock

Cameron

2010

In Vitro Cell.Dev.Biol.-Plant

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Conditions for induction of embryogenic nodules and subsequent somatic embryogenesis in the tropical hardwood Cedrela odorata are described. Embryo explants from ungerminated mature seeds were placed on Driver and Kuniyuki Walnut (DKW) medium using 0.8% w/v agar as the gelling agent, plus benzyladenine (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D). Various phytohormone combinations were tested, from which 5 µM BA + 50 µM 2,4-D were chosen as the standard regime based on a maximum frequency of embryogenic nodule occurrence of 20-25% on this medium. Nodules, when excised from the cotyledons and placed on growth-regulator-free medium, produced both plantlets and secondary embryogenic tissue. With extended culture on growth-regulator-free DKW medium, plantlets developed roots and could be transplanted into pots for further growth. The frequency of nodule initiation could be improved by either orienting the cotyledon explants with their lower (abaxial) surface in contact with the medium or imposing a pre-excision period of heat shock. The treatments together were additive. An optimum heat-shock temperature (47°C) and range of exposure times (8-12 h) were defined.

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Somatic Embryogenesis in Big-leaf Mahogany (Swietenia macrophylla King)

Cited by 12 publications

References 19 publications

Induction of somatic embryogenesis and plant regeneration in the tropical timber tree Spanish red cedar [Cedrela odorata L. (Meliaceae)]

Induction of somatic embryogenesis and plant regeneration in the tropical timber tree Spanish red cedar [Cedrela odorata L. (Meliaceae)]

H2O2 Significantly Affects Larix kaempferi × Larix olgensis Somatic Embryogenesis

Plant regeneration in Spanish cedar, Cedrela odorata L., using zygotic embryo explants from mature seed and improvement of embryogenic nodule initiation by heat shock

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