Plant regeneration in Spanish cedar, Cedrela odorata L., using zygotic embryo explants from mature seed and improvement of embryogenic nodule initiation by heat shock

Abstract

Conditions for induction of embryogenic nodules and subsequent somatic embryogenesis in the tropical hardwood Cedrela odorata are described. Embryo explants from ungerminated mature seeds were placed on Driver and Kuniyuki Walnut (DKW) medium using 0.8% w/v agar as the gelling agent, plus benzyladenine (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D). Various phytohormone combinations were tested, from which 5 µM BA + 50 µM 2,4-D were chosen as the standard regime based on a maximum frequency of embryogenic nodule occurrence of 20-25% on this medium. Nodules, when excised from the cotyledons and placed on growth-regulator-free medium, produced both plantlets and secondary embryogenic tissue. With extended culture on growth-regulator-free DKW medium, plantlets developed roots and could be transplanted into pots for further growth. The frequency of nodule initiation could be improved by either orienting the cotyledon explants with their lower (abaxial) surface in contact with the medium or imposing a pre-excision period of heat shock. The treatments together were additive. An optimum heat-shock temperature (47°C) and range of exposure times (8-12 h) were defined.

Expression analysis of two SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK) genes during in vitro morphogenesis in Spanish cedar (Cedrela odorata L.)

“…plays an important role in the signaling process during embryo formation (Talapatra et al 2014). Therefore, expression analyses of this gene in recalcitrant species, such as Spanish cedar, are valuable for studying its use as a potential marker in somatic embryogenesis (Cameron 2010;Liu et al 2018). SERKs belong to a small gene family of receptor-like kinases involved in signal transduction (Singh and Khurana 2017).…”

“…There are two reports of SE from zygotic embryo in Cedrela, one study applies 3 mg/L dicamba for SE induction (Peña-Ramírez et al 2011), and the other report applies different concentrations of 2,4-D and BAP combined with a thermal shock (Cameron 2010). However, there are no studies on the molecular mechanisms of the SE in C. odorata (aan den Toorn 2015).…”

PROPAGACION in vitro DE SEGMENTOS NODALES DE CEDRO (Cedrela odorata L.) OBTENIDOS A PARTIR DE SEMILLAS BOTANICAS

“…Aunque la literatura reporta escasos reportes de la existencia de diferentes formas de resistencia en Meliaceae al barrenador de la yema Hypsiphylla grandella, con particular énfasis en los trabajos desarrollados en Costa Rica con Cedrela odorata y Swietenia macrophylla muestra significativa existencia de resistencia genética al barrenador en estas especies arbóreas (Patiño, 1997). En los últimos años las técnicas biotecnológicas han contribuido a la generación de una metodología de desarrollo in vitro, así como avances en estudios de propagación vía organogénesis (Pérez et al, 2002;Pérez et al 2006;García-Gonzáles et al, 2011) y embriogénesis somática (Muñoz, 2003;Daquinta et al, 2004;Cameron, 2010) de Cedrela odorata. También se han realizado estudios en la propagación in vitro de Cedrela fissilis (Da Costa-Nunes et al, 2001;Vila et al, 2009).…”

Meristematic Nodule: a Valuable Developmental Pathway for Plant Regeneration

“…; 或在培养过程中发生脱分化, 产生胚性愈 伤组织, 进而间接再生体细胞胚 (Chaudhuri et al, 2004;Sané et al, 2006;Cameron, 2010) et al, 1998) et al, 1991;Ito et al, 1996;Godo et al, 1998;Xie and Hong, 2001;Schestibratov et al, 2003;Kongbangkerd et al, 2005;Alves et al, 2006)…”