Somatic embryogenesis in Araucaria angustifolia

Santos, André Luis Wendt dos; Steiner, Neusa; Guerra, Miguel Pedro; Zoglauer, Kurt; Moerschbacher, Bruno M.

doi:10.1007/s10535-008-0044-1

Cited by 36 publications

(25 citation statements)

References 26 publications

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“…Here, we investigated the role of both NSCs and the structural carbohydrates of the cell wall during A . angustifolia embryogenesis, comparing a cell line that was responsive to ABA, a blocked cell line, and the globular stage of zygotic embryogenesis, since the two cell lines were induced from immature zygotic embryos [ 84 ].…”

Section: Discussionmentioning

confidence: 99%

Carbohydrate-mediated responses during zygotic and early somatic embryogenesis in the endangered conifer, Araucaria angustifolia

et al. 2017

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Three zygotic developmental stages and two somatic Araucaria angustifolia cell lines with contrasting embryogenic potential were analyzed to identify the carbohydrate-mediated responses associated with embryo formation. Using a comparison between zygotic and somatic embryogenesis systems, the non-structural carbohydrate content, cell wall sugar composition and expression of genes involved in sugar sensing were analyzed, and a network analysis was used to identify coordinated features during embryogenesis. We observed that carbohydrate-mediated responses occur mainly during the early stages of zygotic embryo formation, and that during seed development there are coordinated changes that affect the development of the different structures (embryo and megagametophyte). Furthermore, sucrose and starch accumulation were associated with the responsiveness of the cell lines. This study sheds light on how carbohydrate metabolism is influenced during zygotic and somatic embryogenesis in the endangered conifer species, A. angustifolia.

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Section: Discussionmentioning

confidence: 99%

Carbohydrate-mediated responses during zygotic and early somatic embryogenesis in the endangered conifer, Araucaria angustifolia

et al. 2017

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“…As we also report in our system here, promotivemitogenic factors are released in conditioned medium. 64 For example, phytosulfokine a, 21,22 cell wall remodeling enzymes, [65][66][67][68] and parietal fragments such as AGPs, [31][32][33][34][35][36][37]69 or oligogalacturonide pectic fragments have been reported. 68 The use of CM obtained from wild type carrot cell suspension favored embryo development in the thermosensitive mutant ts11 that normally produced somatic embryo arrested at the globular stage.…”

Section: 63mentioning

confidence: 99%

Pretreatments, conditioned medium and co-culture increase the incidence of somatic embryogenesis of different Cichorium species

Couillerot

Windels

Vazquez

et al. 2012

Plant Signaling & Behavior

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Somatic embryogenesis (SE) in Cichorium involves dedifferentiation and redifferentiation of single cells and can be induced by specific in vitro culture conditions. We have tested the effect of various treatments on the incidence of SE (ISE) of an interspecific embryogenic hybrid (C. endivia x C. intybus) and of different commercial chicories (C. endivia and C. intybus) that are typically recalcitrant to SE in standard culture conditions. We found that the ISE of the hybrid is significantly increased by pretreatment of tissues by submersion in solutions of glycerol, abscisic acid, spermine, putrescine or of combinations of these compounds. Interestingly, the most efficient of these pretreatments also had an unexpectedly high effect on the ISE of the C. intybus cultivars. The ISE of the hybrid and of the commercial chicories were increased when explants were co-cultured with highly embryogenic chicory explants or when they were cultured in conditioned medium. These observations established that unidentified SE-promoting factors are released in the culture medium. HPLC analyses of secreted Arabino-Galactan Proteins (AGPs), which are known to stimulate SE, did not allow identifying a fraction containing differentially abundant AGP candidates. However, pointing to their role in promoting SE, we found that the hybrid had a drastically higher ISE when amino sugars and L-Proline, the putative precursors of secreted AGPs, were both added to the medium.

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“…Essas moléculas hidrolisam eficientemente a quitina, presente no citoesqueleto da hifa fúngica (Ponstein et al, 1994). Na fisiologia das plantas, essas enzimas estão envolvidas na nodulação em raízes, senescência, embriogênese, florescimento, germinação de sementes e amadurecimento de frutos (Margis-Pinheiro et al, 1994;Hamel et al, 1997;Robinson et al, 1997;Lers et al, 1998;Helleboid et al, 2000;Santos et al, 2008). Experimentos envolvendo plantas que superexpressam quitinases mostraram que folhas de cacaueiro e plantas de fumo transgênicas eram mais resistentes a Colletotrichum gloeosporioides e Ralstonia solanacearum, respectivamente, do que linhagens selvagens (Dana et al, 2006;Maxinova et al, 2006).…”

Section: Introductionunclassified

Efeito do extrato da alga marinha <em>Ascophyllum nodosum</em> e do fosfito de potássio na morfofisiologia do fungo <em>Colletotrichum gloeosporioides</em>, na indução de resistência em mangas \'Tommy Atkins\' contra a antracnose e em características físicas e químicas desses frutos

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Pelo apoio incondicional, amor desmedido e por serem os melhores pais desse mundo! Aos meus irmãos, Alexandre de Melo e Fernanda de Melo Pelo amor que nos une, amizade e suporte ao longo de toda a minha vida! Ao meu avô (in memoriam), José Raimundo Anchieta Que partiu dizendo a todos, com o peito cheio de orgulho, que eu havia mudado para São Paulo só para fazer um curso. Estou de volta, vovô! O curso acabou! À minha avó, Rosa Maria Brito Que sempre esteve ao meu lado, em todas as decisões da minha vida! A Stênio Bruzaca, Que me inunda todos os dias com amor, carinho e companheirismo! A todos vocês, dedico. 4 AGRADECIMENTOS Há muito o que agradecer, especialmente porque em mim, é clara a certeza de que ninguém faz um doutorado sozinho. Primeiramente, agradeço a Deus pela minha vida, pelo cuidado diário e pelas oportunidades incríveis que Ele me proporciona. Eu não sei o que é sonhar e não concretizar e isso tudo acontece sob a permissão do Pai, por isso, muito obrigado. Agradeço, especialmente, ao meu orientador, o Prof. Dr. Sérgio Florentino Pascholati, pela amizade, dedicação, confiança e por ser um exemplo de profissional e ser humano. Por mostrar, para todos os seus orientados, que podemos ser professores e pesquisadores de sucesso, sem perder valores que, mesmo não estando no Lattes, são mais importantes do que qualquer grande descoberta. Aos meus pais, Roger de Melo e Sílvia de Melo, e aos meus irmãos, Alexandre de Melo e Fernanda de Melo, por andarem ao meu lado todos os dias, por apoiarem e respeitarem as minhas escolhas e decisões e por serem a melhor família que eu poderia ter.

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Somatic embryogenesis in Araucaria angustifolia

Cited by 36 publications

References 26 publications

Carbohydrate-mediated responses during zygotic and early somatic embryogenesis in the endangered conifer, Araucaria angustifolia

Carbohydrate-mediated responses during zygotic and early somatic embryogenesis in the endangered conifer, Araucaria angustifolia

Pretreatments, conditioned medium and co-culture increase the incidence of somatic embryogenesis of different Cichorium species

Efeito do extrato da alga marinha <em>Ascophyllum nodosum</em> e do fosfito de potássio na morfofisiologia do fungo <em>Colletotrichum gloeosporioides</em>, na indução de resistência em mangas \'Tommy Atkins\' contra a antracnose e em características físicas e químicas desses frutos

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