Somatic embryogenesis from styles of lemon (Citrus limon)

Carimi, Francesco; Pasquale, Fabio De; Crescimanno, F. G.

doi:10.1007/bf00043619

Cited by 9 publications

(3 citation statements)

References 7 publications

(5 reference statements)

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“…Tissue culture and micropropagation protocols have been described for a number of Citrus spp. using a wide range of explant sources (Grinblat 1972;Barlass and Skene 1982;Duran-Vila et al 1989;Beloualy 1991;Carimi et al 1995;Altaf and Ahmad 1997;Normah et al 1997;Al-Khayri and Al-Bahrany 2001;Khawale and Singh 2005;Ali and Mirza 2006;Altaf et al 2008;Altaf et al 2009a, b;Khan et al 2009;Laskar et al 2009;Sharma et al 2009;Pe'rez-Tornero et al 2010;Singh and Rajam 2009;. However, a little work has been carried out on the tissue culture of C. jambhiri (Raman et al 1992;Altaf and Ahmad 1997;Khawale and Singh 2005;Ali and Mirza 2006;Altaf et al 2008;Sharma et al 2009;Savita et al 2010).…”

Section: Introductionmentioning

confidence: 99%

An efficient plant regeneration protocol from callus cultures of Citrus jambhiri Lush

Savita

Singh

Virk

et al. 2011

Physiol Mol Biol Plants

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Citrus jambhiri Lush. (family Rutaceae), commonly known as 'rough lemon', is one of the favourite rootstocks for lemons, oranges, mandarins, grape fruits and kinnows in Punjab. The present investigation deals with development of an efficient miropropagation protocol for Citrus jambhiri Lush. using cotyledons as explant. Maximum callus induction (91.66 %) was observed on MS medium supplemented with 2,4-D (2 mg/L) in combination with ME (500 mg/L). Green healthy calli were cut into small pieces and cultured on MS medium for regeneration. Maximum shoot regeneration (87.50 %) was observed with BA (3 mg/L). Effect of increasing age of callus was also studied which showed that callus retained regeneration capacity (58.33 %) even after 420 days of culture. Regenerated shoots were separated out and cultured on rooting medium. Maximum rooting response (91.67 %) was observed on half strength MS medium supplemented with NAA (0.5 mg/L). After hardening and acclimatization the plantlet were transferred to the field and showed 67 % survival.

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Section: Introductionmentioning

confidence: 99%

An efficient plant regeneration protocol from callus cultures of Citrus jambhiri Lush

Savita

Singh

Virk

et al. 2011

Physiol Mol Biol Plants

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“…Murcott”. Previous studies used excised nucelli [ 42 ], abortive ovules [ 43 ], unfertilized ovules [ 44 ], undeveloped ovules [ 45 , 46 ], isolated nucellar embryos [ 47 ], juice vesicles [ 48 ], anthers [ 49 ], styles and stigmas [ 50 ], leaves, epicotyls, cotyledons and root segments of in vitro grown nucellar seedling [ 51 ] for somatic embryogenesis in Citrus. We chose undeveloped ovules as callus induction material because previous studies showed that undeveloped ovule is a preferable material for somatic embryogenesis not only for having higher regeneration capacity but also for being mostly virus-free [ 52 ].…”

Section: Resultsmentioning

confidence: 99%

Citrus Cell Suspension Culture Establishment, Maintenance, Efficient Transformation and Regeneration to Complete Transgenic Plant

Moniruzzaman

Huang

Yan³

et al. 2021

Plants

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Agrobacterium-mediated transformation of epicotyl segment has been used in Citrus transgenic studies. The approach suffers, however, from limitations such as occasionally seed unavailability, the low transformation efficiency of juvenile tissues and the high frequency of chimeric plants. Therefore, a suspension cell culture system was established and used to generate transgenic plants in this study to overcome the shortcomings. The embryonic calli were successfully developed from undeveloped ovules of the three cultivars used in this study, “Sweet orange”-Egyptian cultivar (Citrus sinensis), “Shatangju” (Citrus reticulata) and “W. Murcott” (Citrus reticulata), on three different solid media. Effects of media, genotypes and ages of ovules on the induction of embryonic calli were also investigated. The result showed that the ovules’ age interferes with the callus production more significantly than media and genotypes. The 8 to 10 week-old ovules were found to be the best materials. A cell suspension culture system was established in an H+H liquid medium. Transgenic plants were obtained from Agrobacterium-mediated transformation of cell suspension as long as eight weeks subculture intervals. A high transformation rate (~35%) was achieved by using our systems, confirming BASTA selection and later on by PCR confirmation. The results demonstrated that transformation of cell suspension should be more useful for the generation of non-chimeric transgenic Citrus plants. It was also shown that our cell suspension culture procedure was efficient in maintaining the vigor and regeneration potential of the cells.

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“…Somatic embryogenesis is a powerful biotechnological tool for the propagation and genetic improvement of plants. In Citrus , the production of embryogenic callus lines was first reported by Rangan et al [ 42 ] from excised nucelli culture, the regeneration of somatic embryos from stigma and style cultures was first reported by Carimi et al [ 43 ] and, since then, somatic embryogenesis has been induced directly or indirectly via callus formation in several Citrus species to produce plants for mass propagation, breeding program [ 6 ] and virus-free plants [ 44 , 45 , 46 , 47 , 48 , 49 ]. Even if somatic embryogenesis is widely used, little information is available on the behavior of Citrus plants regenerated in vitro from stigma/style and nucellar culture when grafted on rootstock and transferred under greenhouse conditions.…”

Section: Discussionmentioning

confidence: 99%

Different Cell Types Affect the Transition from Juvenile to Mature Phase in Citrus Plants Regenerated through Somatic Embryogenesis

Catalano

Abbate

Bosco

et al. 2022

Plants

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Robust protocols for the regeneration of somatic embryos in vitro are essential for the efficient use of the most modern biotechnologies. Unfortunately, in perennial trees such as Citrus, plants regenerated from juvenile tissues usually exhibit strong, undesirable juvenile characters such as thorny habit and delayed flowering and fruit production. In this work, we tested whether the cell types (nucellar and stigma/style) used to regenerate Citrus plants through somatic embryogenesis affected the transition from the juvenile to mature phase. The results show that regenerants from nucellar cells presented persistent juvenile characters, whereas plants originating from stigma/style explants transited to the mature phase more rapidly. Our observations support the hypothesis that the totipotent cells originated from different cell types are not equivalent, possibly by maintaining memory of their previously differentiated state.

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Somatic embryogenesis from styles of lemon (Citrus limon)

Cited by 9 publications

References 7 publications

An efficient plant regeneration protocol from callus cultures of Citrus jambhiri Lush

An efficient plant regeneration protocol from callus cultures of Citrus jambhiri Lush

Citrus Cell Suspension Culture Establishment, Maintenance, Efficient Transformation and Regeneration to Complete Transgenic Plant

Different Cell Types Affect the Transition from Juvenile to Mature Phase in Citrus Plants Regenerated through Somatic Embryogenesis

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