Somatic embryogenesis from seeds in a broad range of Vitis vinifera L. varieties: rescue of true-to-type virus-free plants

Pedro, Tania San; Gammoudi, Najet; Peiró, Rosa; Olmos, Antonio; Gisbert, Carmina

doi:10.1186/s12870-017-1159-3

Cited by 17 publications

(24 citation statements)

References 38 publications

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“…Young K. paniculata seeds (Figure 1a) used as initial material have great potential for the induction of embryogenic calluses. Similar studies of other species have indicated that seeds are a good choice for embryogenic callus induction [29,34,49,50]. In addition, the genotype also has an effect on embryogenic callus induction rates.…”

Section: Other Factors Influencing Somatic Embryogenesismentioning

confidence: 62%

“…Histological analyses of embryogenic calluses and somatic embryos were performed according to [21]. Plant materials were fixed in a fixative solution (formaldehyde:glacial acetic acid:alcohol, 8:1:1), and then dehydrated using graded ethanol (50,70,85,95, and 100% v/v). Processed materials were embedded in paraffin wax and cut using a microtome device (model no.…”

Section: Histologymentioning

confidence: 99%

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High-Efficiency Somatic Embryogenesis from Seedlings of Koelreuteria paniculata Laxm.

Yang

Guo

et al. 2018

Forests

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Research Highlights: In the current study, we established a method for plant regeneration via somatic embryogenesis (SE) in Koelreuteria paniculata Laxm. for the first time. Background and Objectives: K. paniculata is an important ornamental and medicinal plant in China. However, the plant has difficulty with asexual reproduction, which imposes a limitation on large-scale propagation. Materials and Methods: Embryogenic calluses were induced from stems of aseptic seedlings on induction media. The effects of different media types and concentrations of N6-benzyladenine (BA), α-naphthaleneacetic acid (NAA), and 2,4-dichlorophenoxyacetic acid (2,4-D) on callus induction were examined. Embryogenic calluses were then transferred to Driver-Kuniyuki Walnut (DKW) media containing NAA (0.1–0.2 mg L−1) or 2,4-D (0.5–2.0 mg L−1) to develop somatic embryos. Cotyledon embryos were cultured on DKW media containing NAA (0.1–0.2 mg L−1) until maturation, and were then transferred to 1/2 DKW medium supplemented with 1.0 mg L−1 indole-3-butyric acid (IBA) to produce complete plants. The effects of IBA and NAA on rhizogenesis were then examined by clonal culture. Results: The maximum callus induction frequency (80.25%) was obtained on DKW medium supplemented by 0.5 mg L−1 BA, 0.25 mg L−1 NAA, and 1.5 mg L−1 2,4-D. NAA had a more pronounced effect on somatic embryo growth than did 2,4-D, with a maximum SE frequency (54.75%) observed with 0.1 mg L−1 NAA added to DKW medium. For clonal culture, the highest rooting rate (52%) was observed on 1/4 DKW medium containing 1.5 mg L−1 IBA. Histology studies confirmed the presence of embryogenic calluses and somatic embryos in different stages. Conclusions: This protocol provides a novel method for large-scale propagation of K. paniculata, and creates opportunities for genetic engineering in this species.

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Section: Other Factors Influencing Somatic Embryogenesismentioning

confidence: 62%

Section: Histologymentioning

confidence: 99%

High-Efficiency Somatic Embryogenesis from Seedlings of Koelreuteria paniculata Laxm.

Yang

Guo

et al. 2018

Forests

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“…The result obtained for GFkV was expected because this virus is not seed transmitted [75]. With respect to GLRaV-3, it is not clear if it is present in seeds [34,75], but we have found this virus in some regenerated plants resulting from somatic embryos induced from seeds of other infected grapevines, which would indicate its presence in the seeds [54]. Induction of somatic embryos from stamens or pistils was also reported in grapevine to cure plants of GLRaV [76,77].…”

Section: Virus Analysis and Sanitationmentioning

confidence: 59%

“…As occurred in other areas, grapevine cultivars were found commonly infected by the viruses GFLV, GLRaV, and GFkV. Sanitation of cultivars to be preserved in vitro is being carried out through meristem culture, although other alternative sanitation procedures are being developed [42,54]. In this work, we report the SSR profiles of different accessions of the historic variety "Valencí Blanc" or "Beba" and the historic and endangered variety "Esclafagerres" (or "Esclafacherres/is").…”

Section: Richness Of Grapevine Germplasm In the Comunitat Valencianamentioning

confidence: 99%

Recovering Ancient Grapevine Varieties: From Genetic Variability to In Vitro Conservation, A Case Study

Gisbert¹,

Peiró²,

Pedro³

et al. 2018

Grapes and Wines - Advances in Production, Processing, Analysis and Valorization

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A great number of varieties have been described in grapevine; however, few of them are currently in use. The increasing concern on varietal diversity loss has encouraged actions for recovering and preserving grapevine germplasm, which represents valuable resources for breeding as well as for diversification in grapevine-derived products. On the other hand, it is expected that this important crop, which is distributed in warm areas worldwide, will suffer the climate changes. Therefore, it is also convenient the identification of intravarietal variability and the recovery of accessions well adapted to particular environments. In this chapter, we will contribute to highlight the importance of recovering ancient materials, the usefulness of SSR markers to determine their molecular profile, the importance to analyze their virus status, and the possibilities that offer biotechnological tools for virus sanitation and in vitro storage as a complement of field preservation. In this context, we have evaluated different grapevine accessions and developed in vitro culture protocols for micropropagation, sanitation, and storage grapevine cultivars. In this work, we report the results obtained for the historic variety "Valencí Blanc" (or "Beba") and the historic and endangered variety "Esclafagerres" ("Esclafacherres" or "Esclafacherris").

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“…As occurred in other areas, grapevine cultivars were found commonly infected by the viruses GFLV, GLRaV and GFkV. Sanitation of cultivars to be preserved in vitro is being carried out through meristem culture, although other alternative sanitation procedures are being developed [42,54]. In this work, we report the SSR profiles of different accessions of the historic variety 'Valencí Blanc' or 'Beba and the historic and endangered variety 'Esclafagerres' (or 'Esclafacherres/is').…”

Section: Richness Of Grapevine Germplasm In the Comunitat Valencianamentioning

confidence: 99%

Desarrollo y aplicación de técnicas biotecnológicas para el saneamiento, multiplicación, caracterización y conservación de germoplasma de vid (Vitis vinifera L.)

Galán¹,

María²

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Grapevine is a crop of vegetative propagation with great economical importance which is distributed worldwide in temperate zones. It is mainly used for wine and table grape production. Despite the fact thousands of varieties exist only few are commonly used, producing a loss of variability. Since the arrival of the phylloxera aphid the grapevine culture is performed using resistant rootstocks. The Directive Commission 2005/43/EC amending the Directive Council annexes 68/193/EEC, indicates that grapevine material should be free of these viruses: Grapevine fanleaf virus (GFLV), Grapevine leafroll associated virus 1 (GLRaV-1), Grapevine leafroll associated virus 3 (GLRaV-3), Grapevine fleck virus (GFkV) and Arabis mosaic virus (ArMV). In this work different methodologies related with in vitro culture were evaluated and developed with the aim of sanitate, mutiplicate and preserve grapevine germplam. In vitro preservation of grapevine varieties is a complementary stratregy of field preservation. In a preservation program it is important the selection of the starting material, determine its sanitary status, sanitate if it is necessary and, establish the appropriate in vitro conditions for its preservation. In the first chapter of this thesis (Chapter 1) the assays carried out for inducing somatic embryogenesis in 16 grapevine varieties which include six that were infected by the viruses GFLV, GLRaV-3 and GFkV are described. In grapevine, somatic embryogenesis has been described as a usefull methodology for virus sanitation and is the common pathway to adventitious regeneration. Efficient regeneration prototocols are necessary to address breeding by genetic transformation. In this work, cut seed are used as starting material and virus-free plants and high precentages of regeneration were obtained in all the varieties evaluated. The use of microsatellite markers has been useful to select those plants with the same genotype than the mother plant. From the other side, in this work, a micropropagation protocol starting from a healthy plant of the cultivar 'Monastrell' was developed and high multiplication rates with good adaptation to field conditions were obtained (Chapter 2). The culture medium MW was evaluated in 16 grapevine varieties as well as the following modifications: reduction in a half of the sugar content and elimination of the Indolebutyric Acid. The aim of these assays was to determine for each variety which conditions are the most appropiate to maintain in active in vitro culture this germplasm, lengthen the time between subcultures for reducing costs (Chapter 3). In Chapter 4 it is included a divulgation report that summarized the steps followed in the MINECO proyect CGL2015-70843-1.5.1.5. Crioconservación/Crioterapia 1.5.2 Micropropagación X 1.5.3 Conservación in vitro 19 1.5.4 Mejora genética y transformación genética 20 2. Objetivos 39 3. Capítulos 41 Capítulo 1: Somatic embryogenesis from seeds in a broad range of Vitis vinifera L. varieties: rescue of true-to-type virus-free plants 43 Capítulo 2: In vi...

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Somatic embryogenesis from seeds in a broad range of Vitis vinifera L. varieties: rescue of true-to-type virus-free plants

Cited by 17 publications

References 38 publications

High-Efficiency Somatic Embryogenesis from Seedlings of Koelreuteria paniculata Laxm.

High-Efficiency Somatic Embryogenesis from Seedlings of Koelreuteria paniculata Laxm.

Recovering Ancient Grapevine Varieties: From Genetic Variability to In Vitro Conservation, A Case Study

Desarrollo y aplicación de técnicas biotecnológicas para el saneamiento, multiplicación, caracterización y conservación de germoplasma de vid (Vitis vinifera L.)

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