Somatic embryogenesis from flower tepals of Hippeastrum aiming regeneration of virus-free plants

Almeida, Naiara Vallado de; Rivas, Eliana Borges; Cardoso, Jean Carlos

doi:10.1016/j.plantsci.2022.111191

Cited by 19 publications

(12 citation statements)

References 39 publications

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“…Various factors affecting somatic embryogenesis have been identified. These include exogenous hormones [28], AgNO 3 [29], light conditions [30], and abscisic acid (ABA) [31], amongst others. Several studies have shown that incorporation of PEG in media or PEG treatment can effectively facilitate somatic embryogenesis [32,33].…”

Section: Discussionmentioning

confidence: 99%

A Stable and Efficient Genetic Transformation Method for Embryogenic Callus of Fraxinus mandshurica

Dong

Zhao

et al. 2023

Forests

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Fraxinus mandshurica is a widely used greening and ornamental tree species. However, its genetic transformation system has been hampered by problems such as low transformation efficiency, among others, which can hinder research related to molecular breeding and the analysis of functional genes. Thus, in this study, a novel genetic transformation method for efficient transformation of the embryonic callus of Fraxinus mandshurica was investigated. The method was optimized in terms of factors such as antibiotics, infection solution concentrations, co-culture time, and somatic embryo maturation. The results indicated that the optimal antibiotic concentration was 10 mg·L−1 of hygromycin (Hyg). At this point, the callus proliferation multiple was only 0.12. The highest transformation efficiency was found to be 93.93% when the absorbance of the infection solution concentration at OD600 was 0.4. Interestingly, transformation efficiency was found to be highest (77.9%) at 48 h of co-culture, with a GUS staining rate of 88.23%. The medium for somatic embryo maturation of transformed callus was half-strength MS medium (MS 1/2) containing 60 g·L−1 polyethylene glycol, 1 mg·L−1 abscisic acid, 400 mg·L−1 casein enzymatic hydrolysate (CH), 20 g·L−1 sucrose, 1 g·L−1 activated charcoal, and 5 g·L−1 gellan gum. The medium for somatic embryo germination was MS ½, containing 0.2 mg·L−1 of N-(Phenylmethyl)-9H-purin-6-amine(6-BA) and 5.0 mg·L−1 of gibberellin (GA). These results are of significance for the verification of the gene function and future genetic improvement of Fraxinus mandshurica.

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Section: Discussionmentioning

confidence: 99%

A Stable and Efficient Genetic Transformation Method for Embryogenic Callus of Fraxinus mandshurica

Dong

Zhao

et al. 2023

Forests

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“…SEs can be in vitro-induced from a range of plant explant types, such as embryogenic callus tissues, cotyledons, and zygotic embryos, under appropriate culture conditions [9,16,30]. In general, immature tissues and organs are easier to produce SEs compared with old or adult plant explants [31], and immature embryos were the most frequently used explants in somatic embryogenesis [16,19].…”

Section: Factors Affecting Se Inductionmentioning

confidence: 99%

Somatic Embryogenesis Induction and Genetic Stability Assessment of Plants Regenerated from Immature Seeds of Akebia trifoliate (Thunb.) Koidz

Zhang

Cao

Wang

et al. 2023

Forests

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Akebia trifoliata is a perennial woody plant with considerable potential in nutrition, food, and health, and the production of seedlings with high quality is critical for its economic utilization. Plant regeneration through somatic embryogenesis is a powerful alternative for propagating many plant species. In this study, a simple and practicable protocol was developed for plant regeneration from immature seeds of A. trifoliata via somatic embryogenesis, and the genetic stability of regenerated plants was also assessed. In the somatic embryo (SE) induction stage, the highest frequency of somatic embryogenesis (35.2%) was observed on the WPM medium containing 1.0 mg L−1 of thidiazuron (TDZ) and 1.0 mg L−1 of 6-benzyladenine (6-BA). The concentration of 6-BA was optimized at 1.0 mg L−1 for the proliferation and maturation of the induced SEs, and the combination of 2.0 mg L−1 of indole-3-butyric acid (IBA) and 0.5 mg L−1 of TDZ was the most responsive for root development and plant growth. The leaf morphological characteristics greatly varied among the established plants, and they could be grouped into three plant types, namely the normal type, Type Ι, and Type ΙΙ. Remarkable differences in the number, size, shape, and color of the leaflets were observed among the three types, while their ploidy level was the same via flow cytometry analysis. The Type ΙΙ and the Type Ι plants had the highest and the lowest net photosynthesis rate, transpiration rate, and stomatal conductance among the three groups, respectively, and both had a smaller size of stomatal guard cells than the normal type. Simple sequence repeat (SSR) analysis detected that 41 bands (43.62%) were different from those observed in the wild, indicating a high degree of polymorphism between the regenerants and their donor parent. The obtained plants might hold potential for future genetic improvement and breeding in A. trifoliata, and the established regeneration protocol might serve as a foundation for in vitro propagation and germplasm preservation of this crop.

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“…Plantlet regeneration by somatic embryogenesis has been reported in many genera of orchids; for example— Cymbidium [ 103 , 104 , 105 , 106 , 107 , 108 ], Phalaenopsis [ 108 , 109 , 110 , 111 , 112 , 113 , 114 , 115 ], Oncidium [ 28 , 116 , 117 , 118 , 119 , 120 ], Dendrobium [ 121 , 122 , 123 , 124 ], Rhynchostylis [ 125 ], Renanthera [ 126 ], Paphiopedilum [ 127 , 128 ], Malaxis [ 129 , 130 ], Epipactis veratrifolia [ 131 ], Spathoglottis plicata [ 132 ], Geodorum densiflorum [ 133 ], Anoectochilus elatus [ 134 ], and Nothodoritis zhejiangensis [ 135 ]. In addition to orchids, it has also been reported in diverse ornamentals, such as rose [ 136 ], Rosa × damascena [ 137 ], chrysanthemum [ 138 , 139 ], lilies [ 140 , 141 , 142 , 143 , 144 , 145 , 146 ], jasmi...…”

Section: Standard Techniques Involved In Plantlet Generation In Vitromentioning

confidence: 99%

Tissue Culture in Ornamentals: Cultivation Factors, Propagation Techniques, and Its Application

Mumivand

Akter

et al. 2022

Plants

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Ornamentals come in a variety of shapes, sizes, and colors to suit a wide range of climates, landscapes, and gardening needs. Compared to demand, a shortage of plant materials and diversity force the search for solutions for their constant acquisition and improvement to increase their commercial value, respectively. In vitro cultures are a suitable solution to meet expectations using callus culture, somatic embryogenesis, protoplast culture, and the organogenesis of protocorm-like bodies; many of these techniques are commercially practiced. Factors such as culture media, explants, carbohydrates, plant growth regulators, and light are associated with the success of in vitro propagation. Techniques, especially embryo rescue and somatic hybridization, are widely used to improve ornamentals. The development of synthetic seed allows season-independent seed production and preservation in the long term. Despite the advantages of propagation and the improvement of ornamentals, many barriers still need to be resolved. In contrast to propagation and crop developmental studies, there is also a high scope for molecular studies, especially epigenetic changes caused by plant tissue culture of ornamentals. In this review, we have accumulated and discussed an overall update on cultivation factors, propagation techniques in ornamental plant tissue culture, in vitro plant improvement techniques, and future perspectives.

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Somatic embryogenesis from flower tepals of Hippeastrum aiming regeneration of virus-free plants

Cited by 19 publications

References 39 publications

A Stable and Efficient Genetic Transformation Method for Embryogenic Callus of Fraxinus mandshurica

A Stable and Efficient Genetic Transformation Method for Embryogenic Callus of Fraxinus mandshurica

Somatic Embryogenesis Induction and Genetic Stability Assessment of Plants Regenerated from Immature Seeds of Akebia trifoliate (Thunb.) Koidz

Tissue Culture in Ornamentals: Cultivation Factors, Propagation Techniques, and Its Application

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