Somatic embryogenesis and plant regeneration in Cichorium intybus L. (Witloof, Compositae)

Heirwegh, Kristin M. G.; Banerjee, Nirmalya; Nerum, Karel Van; Langhe, Edmond De

doi:10.1007/bf00269219

Cited by 18 publications

(6 citation statements)

References 3 publications

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“…It has been reported that higher concentrations (6%) of sucrose increases somatic embryo maturation in Picea mariana (Trembly and Trembly 1995). There have been several reports of increased somatic embryogenesis on medium containing reduced nutrients (Heirwegh et al 1985;Smith and Krikorian 1989;Nadel et al 1990;Choi and Soh 1996). In the present study we found that media dilution reduces embryo maturation and MS medium dilution up to 50% significantly improved germination.…”

Section: Discussionsupporting

confidence: 56%

Effects of type of explant and age, plant growth regulators and medium strength on somatic embryogenesis and plant regeneration in Eucalyptus camaldulensis

Prakash

Gurumurthi

2009

Plant Cell Tiss Organ Cult

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Plant regeneration was achieved through direct and indirect somatic embryogenesis in Eucalyptus camaldulensis. Callus was induced from mature zygotic embryos and from cotyledon explants collected from 10, 15, 25, and 30-day-old seedlings cultured on Murashige and Skoog (MS) basal medium supplemented with different concentrations of naphthaleneacetic acid (NAA). Maximum callus induction from mature zygotic embryos was obtained on MS basal medium containing 1 mg l -1 NAA. The frequency of callus development varied based on the age of the cotyledon explants 10-day-old explants giving highest percentage on MS basal medium supplemented with 1 mg l -1 NAA. Callus obtained from mature zygotic embryos gave highest frequency of somatic embryogenesis on MS basal medium containing 0.5 mg l -1 benzyladenine (BA) and 0.1 mg l -1 NAA. Separate age wise culture of the calli, obtained from cotyledons of different ages cultured separately, revealed high somatic embryogenic potential on callus from 10-day-old cotyledons. Direct somatic embryogenesis too was obtained from hypocotyl explants without an intervening callus phase on MS basal medium containing 0.5 mg l -1 BA. The effects of abscisic acid (ABA), sucrose, and different strengths of MS medium on somatic embryo maturation and germination were also investigated. Number of mature somatic embryos increased with lower concentrations (0-1 mg l -1 ) of ABA while no significant differences were observed at higher concentrations (2-5 mg l -1 ) of ABA. Compared to basal medium containing lower concentrations of sucrose (1%), the MS medium supplemented with higher levels of sucrose (4%) showed significantly lower frequency of mature somatic embryos. Basal medium without any dilution gave the highest number of immature embryos. However, the number of mature embryos was high at higher medium dilutions.

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Section: Discussionsupporting

confidence: 56%

Effects of type of explant and age, plant growth regulators and medium strength on somatic embryogenesis and plant regeneration in Eucalyptus camaldulensis

Prakash

Gurumurthi

2009

Plant Cell Tiss Organ Cult

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“…Cytokinins have been found useful in fostering somatic embryo maturation (Fujimura & Komamine 1975), and cotyledon development (Ammirato & Steward 1971). The use of 2,4-D and BA for initiation of somatic embryogenesis has been reported for other species of Asteraceae (Heirwegh et al 1985;May & Trigiano 1991). However, in this study, development beyond the globular stage was inhibited by remaining on medium containing these plant growth regulators.…”

contrasting

confidence: 47%

A three media transfer system for direct somatic embryogenesis from leaves ofSenecio x hybridus Hyl. (Asteraceae)

Malueg

McDaniel

Graham

et al. 1994

Plant Cell Tiss Organ Cult

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Bisected leaves were cultured on semi-solid Murashige & Skoog medium amended with 13.5 IxM 2,4-D and 4.5 ixM BA for 7-14 days, transferred to 0.5% activated charcoal medium (without growth regulators) for three days, then transferred to MS basal medium. Control explants remained on initiation medium for comparison to transferred explants. Twenty-eight days after initiation of cultures, explants exposed to 11-14 days on induction medium yielded the largest number and most developmentally advanced embryos. Mature, white somatic embryos from transferred explants were visible after 35 days. Conversely, somatic embryos on control explants were not morphologically mature until 2-4 months. Mature embryos from transferred explants germinated without a resting stage, whereas embryos from control explants appeared quiescent. Histological examination confirmed embryo anatomy, however embryos, regardless of treatment, had abnormal cotyledons and regenerated plants had multiple stems.

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“…However, Staden and Bennett (1991) reported that the tissue from galls induced by Procecidochares utilis on the stems of croftonweed produce callus on a cytokinin-free medium, which resulted from much higher levels of endogenous cytokinin in gall tissue than that in normal stem tissue. In previous reports, (2,4-dichlorophenoxy)acetic acid (2,4-D), alone or combined with BA, was used to induce callus (Chengalrayan and Gallo-Meagher 2001;Ferreira and Handro 1988;Heirwegh et al 1985;Xu and Jia 1996). Different cytokinin and auxin combinations and concentrations had a significant (P , 0.01) effect on callus induction (Tables 1-3).…”

Section: Resultsmentioning

confidence: 99%

In Vitro Culture of Croftonweed (Ageratina Adenophora): Considerable Potential for Fast and Convenient Plantlet Production

Shen¹,

Li²,

Wang³

et al. 2007

Weed Technology

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A callus induction and plantlet regeneration system for croftonweed was developed by studying the influence of explant type (leaf, stem, and nodal segment) and different concentrations of plant growth regulators. The leaf was a better explant for callogenesis compared to the stem. The highest callus induction frequency (87.2%) was obtained from leaf segments on Murashige and Skoog's medium (MS medium) supplemented with 0.5 mg/L (2,4-dichlorophenoxy)acetic acid and 2.0 mg/L 6-benzylaminopurine (BA), and 71.6% differentiation along with a multiplication rate of 4.1 adventitious shoots per callus was achieved with a combination of 0.5 mg/L 1-naphthaleneacetic acid (NAA) and 1.0 mg/L BA. In addition, MS medium supplemented with 0.5 mg/L NAA and 1.0 mg/L BA was the best medium for axillary shoot regeneration from nodal segments. Rhizogenesis of cultured shoots was satisfactorily obtained in half-strength MS without any growth regulators. The regenerated rooted plantlets were successfully acclimatized in soil where they grew normally without showing any morphological variation. These studies provide the prerequisite system for the development of genetic engineering in the future and propagating croftonweed rapidly for further study.

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Somatic embryogenesis and plant regeneration in Cichorium intybus L. (Witloof, Compositae)

Cited by 18 publications

References 3 publications

Effects of type of explant and age, plant growth regulators and medium strength on somatic embryogenesis and plant regeneration in Eucalyptus camaldulensis

Effects of type of explant and age, plant growth regulators and medium strength on somatic embryogenesis and plant regeneration in Eucalyptus camaldulensis

A three media transfer system for direct somatic embryogenesis from leaves ofSenecio x hybridus Hyl. (Asteraceae)

In Vitro Culture of Croftonweed (Ageratina Adenophora): Considerable Potential for Fast and Convenient Plantlet Production

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