In Vitro Culture of Croftonweed (Ageratina Adenophora): Considerable Potential for Fast and Convenient Plantlet Production

Abstract

A callus induction and plantlet regeneration system for croftonweed was developed by studying the influence of explant type (leaf, stem, and nodal segment) and different concentrations of plant growth regulators. The leaf was a better explant for callogenesis compared to the stem. The highest callus induction frequency (87.2%) was obtained from leaf segments on Murashige and Skoog's medium (MS medium) supplemented with 0.5 mg/L (2,4-dichlorophenoxy)acetic acid and 2.0 mg/L 6-benzylaminopurine (BA), and 71.6% differentiation along with a multiplication rate of 4.1 adventitious shoots per callus was achieved with a combination of 0.5 mg/L 1-naphthaleneacetic acid (NAA) and 1.0 mg/L BA. In addition, MS medium supplemented with 0.5 mg/L NAA and 1.0 mg/L BA was the best medium for axillary shoot regeneration from nodal segments. Rhizogenesis of cultured shoots was satisfactorily obtained in half-strength MS without any growth regulators. The regenerated rooted plantlets were successfully acclimatized in soil where they grew normally without showing any morphological variation. These studies provide the prerequisite system for the development of genetic engineering in the future and propagating croftonweed rapidly for further study.

Propagation of goldenrod (Solidago canadensis L.) from leaf and nodal explants

“…In the present study, leaf is a better explant source for the species with its 100% of callus induced in MS medium supplemented with BA and NAA. This result was fairly consistent with other species tested [28,33,37]. The intercalary meristems distributed in leaves might be responsible for the higher regeneration potential.…”

“…However, during root induction, the low concentration of auxin didn't help the rooting when compared to the PGR-free medium. This result is consistent with what was observed on other Asteraceae species [25][26][27][28]. It is proposed that the half-strength MS may be sufficient for satisfactory rooting of goldenrod plants.…”

“…It is confirmed that the balance between NAA and BA is a determining factor for morphogenic variations [41][42][43]. This pattern of BA impact was also observed on other species of Asteraceae [28,37,40]. Previous reports show that somatic embryos or adventitious shoots could be proliferated directly from explants using only 2,4-D, BA, or NAA + BA [40,[44][45][46].…”

“…Most of the earlier researchers found BA to be the most effective cytokinin for inducing shoot development in other species of the Asteraceae as well as in plants of other families [28,[37][38][39][40]. Our observation showed that BA was also significantly effective for proliferation of goldenrod.…”

“…It has been reported that direct plant regeneration from nodal stems explants could be successfully obtained in some Asteraceae plants [25,28], Shen et al [28] reported a number of 4.6 shoots per stem in 0.5 mg/L NAA + 1.0 mg/L BA from the wild growing croftonweed, Borthakur et al [25] reported similar result on Eupatorium adenophorum. In the study, we achieved a significantly better proliferation frequency (7.5 shoots/nodal stem explant) for stem explants, high frequency shoot proliferation was obtained using leaf explants (3.2 shoots Fig.…”