Somatic embryogenesis and plant regeneration in zygotic embryo explant cultures of rugosa rose

Abstract

Rugosa rose (Rosa rugosa) is cultivated as a garden flower and an important genetic resource for the breeding of roses (R. hybrida). This study describes culture conditions for high frequency plant regeneration from zygotic embryo explants via somatic embryogenesis in rugosa rose. Mature zygotic embryo, cotyledon, and radicle explants formed embryogenic calluses at frequencies of 38, 6.7, and 8.8% when cultured on half-strength Murashige and Skoog medium (MS) supplemented with 2.26, 9.05, and 9.05 lM 2,4-dichlorophenoxyacetic acid, respectively. Embryogenic calluses produced numerous somatic embryos, which then developed into plantlets on MS without growth regulators. Regenerated plantlets were grown to whole plants in a growth chamber.

High Frequency in vitro Propagation Through Somatic Embryogenesis of Iphigenia indica Kunth et Benth, an Endangered Medicinal and Colchicines Yielding Herb of Commercial Interest

“…Afterwards, 2,4-D has been used as somatic embryo inducer in a broad range of species (Yu et al 2000, Puchooa 2004, Raghavan 2004. In Liliaceae, 2,4-D was reported to favor somatic embryogenesis in culture as an embryo inducer (Kim et al 2000, Mukhopadhyay et al 2004. In Iphigenia too, formation of friable, nodular and soft em-bryonic callus occurred only in presence of 2,4-D in the medium (Table 2).…”

Indução de embriões somáticos a partir de embriões zigóticos de Heliconia bihai (L.) L. cv. Lobster Claw Two

“…Entretanto, a conversão dos calos em embriões somáticos pode ser induzida in vitro, a partir de tecidos considerados maduros, como embriões zigóticos provenientes de frutos maduros, como foi observado no presente trabalho e, também, em Lonicera japonica Thunb. (Kim et al, 2003). Resultados similares também foram alcançados por Zale et al (2004), os quais observaram que os embriões zigóticos maduros de cultivares de Triticum monococcum L. e T. tauschii (Coss.)…”

Early somatic embryogenesis in Heliconia chartacea Lane ex Barreiros cv. Sexy Pink ovary section explants

“…1B). Cells that demonstrated these characteristics were considered embryogenic cells (Durzan, 1988;Mathew and Philip, 2003;Kim et al, 2003;Sáenz et al, 2006) and staining with acetic carmine indicated the presence of reserve substances that would favor tissue development (Guerra et al, 1999). The combination of IAA and 2,4-D also promoted the development of yellowish nodules, especially in the treatments with 8.56 µM IAA plus 13.57 µM 2,4-D; 11.42 µM IAA plus 22.62 µM 2,4-D; and 14.27 µM IAA plus 22.62 µM 2,4-D. Histological analyses of this callus material revealed heavily stained cells located on the surface of the callus.…”