Somatic cell counts as an indicator of mammary infection in periparturient cows

Reis, Juliana França dos; Leite, Renata F.; Silva, Cynthia Pereira da Costa e; Baccili, Camila Costa; Melville, Priscilla Anne; Benites, Nilson Roberti; Gomes, Viviani

doi:10.11606/issn.1678-4456.v53i2p161-168

Cited by 4 publications

(6 citation statements)

References 10 publications

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“…Overall rate of mammary infection for the transitional period was 25.82%. NAS were predominantly isolated in the mammary secretion of cows during peripartum (69.2%), similar to the frequency of 76.92% observed by Reis et al [17] in the same property selected for the present research, while Odenstsen et al [15] found frequencies of 31.7%, indicating that sanitary management of the property directly impacts the rates and type of bacterial agents that cause mammary infections.…”

Section: Discussionsupporting

confidence: 88%

“…However, as milking progresses, the elimination of these cells, bacteria, and cellular debris accumulated in the mammary secretion and a subsequent decrease in milk cellularity occur. Physiological changes in cellularity in the immediate postpartum compromise the use of the MSCC for the detection of mastitis [17].…”

Section: Discussionmentioning

confidence: 99%

“…Colostrogenesis may be a risk phase for mastitis in immediate postpartum and it is extremely difficult to differentiate physiological from pathological edema of udder, due to the similarity of clinical signs and inviability of tests that estimate the cellularity of mammary secretion in this period [17]. The aim of this study was to characterize physiological and pathological alterations in mammary gland of Holstein cows during transition period.…”

Section: Introductionmentioning

confidence: 99%

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Physiological and Pathological Alterations in the Mammary Gland of Holstein Cows during Transition Period

Ramos

Madureira

Baldacim

et al. 2020

Acta Scientiae. Vet.

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Background: Physiological and immunological adaptations occurred in peripartum period may be responsible for susceptibility to intramammary infections during colostrogenesis. Increase in blood flow and hormones cause physiological edema by accumulation of lymphatic fluid in interstitial space. Specific composition of mammary secretion increases the susceptibility to mastitis. So, the study of physical, cellular and microbiological changes in the mammary gland in this period are fundamental for understanding, diagnosing and adopting prophylactic measures for bovine mastitis. This study aimed to characterize physiological and pathological changes that occur in the mammary gland during transition period.Materials, Methods & Results: Thirteen cows, in second to fourth lactation were followed from 3 weeks pre-calving to 3 weeks post-calving. Cows were submitted weekly to physical examination of mammary gland, strip cup test, California Mastitis Test (CMT), Microscopic Somatic Cell Count (MSCC), and bacteriological examination of mammary secretion. Mammary quarters were divided into two groups: BAC-: samples that did not present bacteriological growth throughout transition period; BAC+: samples that presented bacteriological growth in at least two isolates of the same bacterial genus. Regarding physical examination of mammary gland, the parameters localized or generalized alteration in volume, asymmetry, reddish color, reduced skin elasticity, increased temperature, and swollen consistency presented higher frequencies in the BAC- group. On the other hand, infection in mammary quarters of cows from BAC+ group promoted a decrease in frequencies of increased volume, decreased elasticity, and elevated temperature in the mammary gland, in addition to a higher positivity to CMT and higher cellularity. Overall rate of intramammary infection was 25.82%, with non-aureus Staphylococci (NAS) being the predominant agent (69.2%). Maximum peak of bacterial isolates occurred immediately after calving (33.3%). Differences were observed between groups (P = 0.021) for CMT, showing a higher frequency of positive tests in BAC+ group (36.5%) compared to BAC- group (18%). MSCC varied between weeks (P = 0.001), groups (P = 0.001), and between the interaction of the two variables (P = 0.019). Highest median value of MSCC was observed at calving (1.5 × 106 cells/mL), being different (P = 0.001) from the +1, +2, and +3 weeks and the lowest median value of MSCC (0.08×106 cells/mL) was observed in +3 compared to previous weeks (P < 0.05).Discussion: NAS was predominantly isolated in mammary secretions of cows during peripartum, it is commonly found in the skin and canal of the teat and are associated with subclinical mastitis. Peak of mammary infection was observed on calving, probably by the cortisol peak resulting in a stress leucogram and decrease in the functional activity of immune cells. Changes observed in the physical examination of mammary gland of cows from BAC- group are compatible with physiological edema caused by circulatory changes that occurs with the proximity of calving, increasing blood flow necessary for production of colostrum. Alterations in the mammary quarters of cows from BAC+ group may be associated with functional loss of mammary parenchyma or persistent infectious process, characterizing clinical findings of pathological changes during transition period. Mammary quarters of the BAC+ group presented higher percentages of positive reactions to CMT in weeks +1 and +2, and higher MSCC values and are associated with infection of the mammary gland.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Physiological and Pathological Alterations in the Mammary Gland of Holstein Cows during Transition Period

Ramos

Madureira

Baldacim

et al. 2020

Acta Scientiae. Vet.

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“…Altas contagens de células somáticas no colostro podem estar relacionadas com a fase fisiológica dos animais e não necessariamente com um quadro inflamatório, porque as alterações glandulares durante a colostrogenese podem culminar naturalmente com o aumento fisiológico da CCS no primeiro colostro produzido (Reis et al, 2016). Essas alterações resultam do desprendimento natural de células secretoras a partir do alto curso secretório iniciado nesta fase; a baixa proporção e atividade de neutrófilos; e alta proporção e atividade fagocitária de macrófagos (Dang et al, 2009;Reis et al, 2016;Tripaldi et al, 2010).…”

Section: Resultsunclassified

Caracterização físico-química do colostro de búfalas na primeira ordenha pós-parto por meio de absorção infravermelha

Oliveira¹,

Rangel²,

Sales³

et al. 2018

Revista Brasileira De Ciência Veterinária

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Objetivou-se caracterizar o colostro da primeira ordenha de búfalas da raça Murrah utilizando a metodologia não oficial de absorção infravermelha. Para isso, foram colhidas amostras individuais de 42 fêmeas multíparas, por meio de ordenha manual. As amostras foram coletadas em até 12 h após o parto e encaminhadas para determinação de gordura, proteína, lactose, sólidos totais e sólidos não gordurosos por meio de absorção infravermelha. Realizamos também a contagem de células somáticas (CCS) e leitura de condutividade elétrica (CE) do colostro. Os dados foram submetidos à análise estatística descritiva através do procedimento proc means. Os valores de condutividade elétrica foram divididos em quatro classes e as médias das características físico-químicas das diferentes classes foram comparadas pelo teste T com 5% de significância. Também foi realizada análise de correlação entre as características químicas, CCS e CE do colostro. Foram obtidas médias de 4,

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“…A presença de microrganismos na secreção colostral observada imediatamente após o parto foi relatada por vários autores (Sargeant et al 2001, Schrick et al 2001, Reis et al 2016. Bacillus cereus (1) Um total de 40 amostras submetidas ao exame bacteriológico (GII e GIII sem crescimento bacteriano).…”

Section: Discussionunclassified

Influência da mastite nas frações eletroforéticas do colostro de vacas da raça Holandesa

et al. 2018

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RESUMO: O objetivo desse estudo foi o de avaliar as frações proteicas em secreções colostrais de vacas acometidas por mastite clínica imediatamente após o parto. Para tanto, foram utilizadas 30 vacas da raça Holandesa distribuídas em três grupos, a saber: Grupo I (GI)- 10 vacas pluríparas sadias, Grupo II (GII) 10 vacas pluríparas que pariram com mastite assintomática e Grupo III (GIII) 10 vacas pluríparas que pariram com mastite clínica. Foram avaliadas as concentrações de imunoglobulina a (IgA), lactoferrina (LF), albumina, imunoglobulina G (IgG), β-lactoglobulina (β-Lg) e α-lactoalbumina (α-La) por meio da eletroforese em gel de poliacrilamida contendo dodecil sulfato de sódio (SDS-PAGE).Observou-se que a IgG, LF e a albumina variaram entre as glândulas com mastite assintomática e clínica quando comparadas às glândulas sadias, e que a presença de um único microrganismo é capaz de promover alterações no proteinograma, com ou sem manifestações clínicas na glândula mamária.

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Somatic cell counts as an indicator of mammary infection in periparturient cows

Cited by 4 publications

References 10 publications

Physiological and Pathological Alterations in the Mammary Gland of Holstein Cows during Transition Period

Physiological and Pathological Alterations in the Mammary Gland of Holstein Cows during Transition Period

Caracterização físico-química do colostro de búfalas na primeira ordenha pós-parto por meio de absorção infravermelha

Influência da mastite nas frações eletroforéticas do colostro de vacas da raça Holandesa

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