Somatic and Germline MicroRNAs Form Distinct Silencing Complexes to Regulate Their Target mRNAs Differently

Dallaire, Alexandra; Frédérick, Pierre-Marc; Simard, Martin J.

doi:10.1016/j.devcel.2018.08.022

Cited by 62 publications

(67 citation statements)

References 67 publications

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“…Although this might suggests that gametic miRNAs increase the stability of transcripts, similar as previously observed in C . elegans 48 , our PARE data indicates that most probably the miRNA targeting events identified here induce the cleavage of their target mRNAs (Figure 4c), showing that developmental miRNA targets in pollen are expressed at high levels and miRNA targeting dampens their expression (Figure 7a-c, model shown in Supplementary Figure 8). As a proof of concept, we analyzed the defects in pollen grain germination experienced by two miRNA target genes identified in our analysis: SKS12 and EIN3 (Figure 4d-g).…”

Section: Discussionmentioning

confidence: 68%

“…We also detected pollen-specific targeting events like the targeting of SKU5 SIMILAR 12 ( SKS12) by miR5021 or ETHYLENE-INSENSITIVE3 ( EIN3 ) by miR5015 (Supplementary Figure 3 and Supplementary Table 4). In other organisms, miRNA targeting in the gametes increases the stability of targeted transcripts 48 . We explored if a similar scenario may apply to Arabidopsis .…”

Section: Resultsmentioning

confidence: 99%

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MicroRNA function transitions from regulating developmental genes to transposable elements during the maturation of pollen

Oliver

Annacondia

Wang

et al. 2019

Preprint

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AbstractmicroRNAs play important roles to control the development of eukaryotic organisms. Both animal and plant microRNAs are essential for the spatio-temporal regulation of development but together with this role, plant microRNAs also control transposable elements and stimulate the production of epigenetically-active small interfering RNAs. This last role is evident in the plant male gamete containing structure, the male gametophyte or pollen grain, but how the dual role of plant microRNAs is integrated during its development is unknown. Here, we provide a detailed analysis of microRNA dynamics during pollen development and their genic and transposable element targets using small RNA and mRNA cleavage (PARE) high-throughput sequencing. Furthermore we uncover the microRNAs loaded in the two main Argonaute proteins in the mature pollen grain, AGO1 and AGO5. Our results indicate that the developmental progression from microspore to mature pollen grain is characterized by a reprogramming from microRNAs focused on the control of development to microRNAs regulating transposable element control.

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Section: Discussionmentioning

confidence: 68%

Section: Resultsmentioning

confidence: 99%

MicroRNA function transitions from regulating developmental genes to transposable elements during the maturation of pollen

Oliver

Annacondia

Wang

et al. 2019

Preprint

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“…Frontiers in Molecular Biosciences | www.frontiersin.org mTOR signaling, and neuronal activity (Bhattacharyya et al, 2006;Banerjee et al, 2009;Jafarifar et al, 2011;Muddashetty et al, 2011;Sosanya et al, 2013). Cell-specific regulation of miRISC composition was recently demonstrated by work from Simard's lab (Dallaire et al, 2018). The structure of the miRNA binding site on mRNA also regulates the miRISC (Zhang K. et al, 2018).…”

Section: Diverse Mirisc Composition and Its Regulation Brings About Tmentioning

confidence: 99%

“…The rest of the miRISC comprises of a diverse set of proteins, such as mRNA degradation machinery components, scaffolding proteins, and many different RNA binding proteins (RBPs), which assemble with the core miRISC to form compositionally diverse miRISCs. This composition, in turn, appears to be a crucial determinant of the kinetics and the outcome of the miRISC function (Dallaire et al, 2018).…”

Section: The Dynamic Nature Of Mirisc Function and Its Potential For mentioning

confidence: 99%

The Role of Dynamic miRISC During Neuronal Development

Nawalpuri

Ravindran

Muddashetty

2020

Front. Mol. Biosci.

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Activity-dependent protein synthesis plays an important role during neuronal development by fine-tuning the formation and function of neuronal circuits. Recent studies have shown that miRNAs are integral to this regulation because of their ability to control protein synthesis in a rapid, specific and potentially reversible manner. miRNA mediated regulation is a multistep process that involves inhibition of translation before degradation of targeted mRNA, which provides the possibility to store and reverse the inhibition at multiple stages. This flexibility is primarily thought to be derived from the composition of miRNA induced silencing complex (miRISC). AGO2 is likely the only obligatory component of miRISC, while multiple RBPs are shown to be associated with this core miRISC to form diverse miRISC complexes. The formation of these heterogeneous miRISC complexes is intricately regulated by various extracellular signals and cell-specific contexts. In this review, we discuss the composition of miRISC and its functions during neuronal development. Neurodevelopment is guided by both internal programs and external cues. Neuronal activity and external signals play an important role in the formation and refining of the neuronal network. miRISC composition and diversity have a critical role at distinct stages of neurodevelopment. Even though there is a good amount of literature available on the role of miRNAs mediated regulation of neuronal development, surprisingly the role of miRISC composition and its functional dynamics in neuronal development is not much discussed. In this article, we review the available literature on the heterogeneity of the neuronal miRISC composition and how this may influence translation regulation in the context of neuronal development.

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“…miRNAs are small noncoding RNAs that target downstream mRNAs for gene regulation (Hammond, ). miRNAs are 21 to 22 nucleotides in length and bind to the 3′ untranslated region (UTR) of a target mRNA and then either degrade (Behm‐Ansmant, Rehwinkel, & Izaurralde, ) or protect (Dallaire, Frederick, & Simard, ) it or inhibit its translation (Kiriakidou et al., ). miRNAs are generated through a canonical biogenesis pathway (Fig.…”

Section: Commentarymentioning

confidence: 99%

In Situ Hybridization for Detecting Mature MicroRNAs In Vivo at Single‐Cell Resolution

Minogue

Arur

2019

CP Molecular Biology

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MicroRNAs (miRNAs) are key regulators of cell and tissue development. However, spatial resolution of miRNA heterogeneity and accumulation patterns in vivo remains uncharted. Next‐generation sequencing methods assay miRNA abundance in tissues, yet these analyses do not provide spatial resolution. A method to assay miRNA expression at single‐cell resolution in vivo should clarify the cell‐autonomous functions of miRNAs, their roles in influencing the cellular microenvironment, and their perdurance and turnover rate. We present an in situ hybridization protocol to map miRNA subcellular expression in single cells in vivo in four days. Using this protocol, we mapped distinct miRNAs that accumulate in the cytoplasm of one sibling oocyte but not another, dependent on the oocyte developmental stage. Thus, this method provides spatial and temporal resolution of the heterogeneity in expression of miRNAs during Caenorhabditis elegans oogenesis. This protocol can generally be adapted to any tissue amenable to dissection and fixation. © 2019 by John Wiley & Sons, Inc.

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Somatic and Germline MicroRNAs Form Distinct Silencing Complexes to Regulate Their Target mRNAs Differently

Cited by 62 publications

References 67 publications

MicroRNA function transitions from regulating developmental genes to transposable elements during the maturation of pollen

MicroRNA function transitions from regulating developmental genes to transposable elements during the maturation of pollen

The Role of Dynamic miRISC During Neuronal Development

In Situ Hybridization for Detecting Mature MicroRNAs In Vivo at Single‐Cell Resolution

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