“…It usually varies over a wide range of traits depending on the instability of the genome and the specific in-vitra culture conditions (De Klerk, 1990). In some species, SV occurs in high frequencies, for example up to 80% in rice (Buiatti and Gimelli, 1993). The comparison of mutations obtained as a result of tissue culture effects in contrast to chemical mutagenesis in tomato (Gavazzi et al, 1987) or treatment with gamma-rays in maize (Novak et al , 1988), indicated a higher frequency of mutants due to SV as weIl as a somewhat different mutation spectrum.…”