Cryopreservation of Germplasm of Tomato

Grout, B. W. W.; Crisp, P.

doi:10.1007/978-3-662-03096-7_26

Cited by 10 publications

(13 citation statements)

References 13 publications

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“…In such conditions the metabolic activity of cells is reduced, which enables their preservation. Despite refrigerated collections allow for a short-or medium-term (few-year) storage of numerous accessions at reduced space (Robertson and Labate 2006;Gonçalves et al 2008a, b), a significant depletion of tomato seeds from stored accessions is observed because of the intermittent viability testing, as well as for the regeneration of plants (Grout and Crisp 1995;Bauchet and Causse 2012). Due to regeneration of small samples from each accession, genetic drift and loss of alleles take place.…”

Section: Refrigeration Of Working and Active Seed Collectionsmentioning

confidence: 99%

“…Therefore, the sole requirement for successful long-term preservation is drying in a stream of air or over silica gel to a level between 5.5 and 18.5% prior to storage. Lower water content reduces the seeds viability, whereas above 18.5% moisture, the High Moisture Freezing Limit makes low-temperature preservation unsuccessful (Grout and Crisp 1995). One should keep in mind, though, that water content, and the life span of stored accessions, can vary greatly (by over 300%) in tomato seeds from the same locality from year-to-year, and also for material from the same locality, within any one season (FAO 2014).…”

Section: Seed Freezing and Cryopreservationmentioning

confidence: 99%

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Managing plant genetic resources using low and ultra-low temperature storage: a case study of tomato

Kulus

2019

Biodivers Conserv

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Ex situ preservation of plant genetic resources is essential. Tomato is one of the most important vegetable crops on the market. However, the genetic diversity of the clade is limited and suffering from genetic erosion phenomenon. Genebanks experience alleles loss on regeneration of small samples, genetic drift, and somaclonal variation in in vitro cultures. Therefore, the development of more efficient ex situ preservation protocols is required. Storage of accessions at low temperatures allows for the reduction of cell metabolic activity and medium or even long-term preservation. Working and active collections of tomato seeds can be stored at + 5 °C, at reduced humidity. Medium-term storage of seeds and pollen can be performed at freezing temperatures (− 20 °C or − 80 °C). This, however, is highly limited as it requires special freezers and can affect the fecundity of the specimens. As for long-term storage, cryopreservation in liquid nitrogen (− 196 °C to c.a. − 140 °C) is also effective. Over time, several cryopreservation techniques have been successfully applied with tomato pollen, seeds and shoot tips, including: slow cooling (not common anymore), desiccation, encapsulation-dehydration, droplet-vitrification and V-cryo-plate. Despite those studies reported high survival and no morphological variation of cryopreservation-recovered shoots, some differences between cryopreserved and noncryopreserved samples, revealed by biochemical, ultrastructural and molecular analyses, were observed. The intensity of those alternations was depending on the cell type, cultivar or plant generation. In the future, more attention could be focused on cryoprotection of embryogenic tissues and application of novel cryopreservation techniques, e.g. vacuum infiltration vitrification.

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Section: Refrigeration Of Working and Active Seed Collectionsmentioning

confidence: 99%

Section: Seed Freezing and Cryopreservationmentioning

confidence: 99%

Managing plant genetic resources using low and ultra-low temperature storage: a case study of tomato

Kulus

2019

Biodivers Conserv

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“…Droplet freezing on aluminum foil; 15% DMSO Grout and Crisp 1995 Controlled rate cooling, various speed steps Kartha et al 1982;Escobar et al 1997Vitrification PVS2 Charoensub et al 1999Escobar et al 2000;Ng and Ng 2000 Freezing with filter paper wrapped in aluminium foil, 10% DMSO…”

Section: Bajaj 1995amentioning

confidence: 99%

“…DMSO at 15% is applied for Brassica napus (Withers et al 1988), chicory (Demeulemeester et al 1992), mint (Towill 1988), and chrysanthemum (Ahn 1995). DMSO is increased stepwise in tomato (Grout and Crisp 1995) and used with glucose in Brussels sprouts (Harada et al 1985) and carnation (Fukai PVS2 (Sakai et al 1990) is the most commonly used vitrification solution and may be used at room temperature or at 0°C. PVS2 is used in plastic cryotubes or in drops on aluminum foil for faster vitrification (See Chap.…”

Section: Plant Species Technique Referencementioning

confidence: 99%