Cryopreservation of Herbaceous Dicots

Keller, E. R.; Senula, Angelika; Kaczmarczyk, Anja

doi:10.1007/978-0-387-72276-4_12

Cited by 21 publications

(3 citation statements)

References 55 publications

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“…e conservation of shoot tips in LN has been recognized as an important method for the long-term storage of potato germplasm (see reviews by Kaczmarczyk et al, 2011;Keller et al, 2008). Studies on the cryopreservation of potato shoot tips started in the late 1970s, and since then, different methods have been developed using several cooling approaches, including two-step cooling (Bajaj, 1977), encapsulation-dehydration (Bouafia et al, 1996), PVS2 vitrification (Sakai et al, 1990), droplet vitrification (Kim et al, 2006), and the DMSO droplet method (Schäfer-Menuhr et al, 1996).…”

Section: Solanum Tuberosum Lmentioning

confidence: 99%

Cryopreservation of Plant Tissues in Poland: Research Contributions, Current Status, and Applications

et al. 2022

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Cryopreservation of vegetatively propagated plant material is an increasingly widely used method for the efficient and safe storage of germplasm resources around the world. In Poland, there are currently four cryobanks in use for long-term plant protection programs. However, plant tissues propagated in vitro constitute only a small portion of the accessions stored in them. To date, cryogenic storage techniques have been developed and adopted in this country for ornamental plants (roses, chrysanthemums, and geophytes), crop species (potato and garlic), forest tree species (the genera Quercus and Fraxinus ), and some ferns. Polish researchers have used suspension cultures of Gentiana spp. and shoot tips of Lamprocapnos spectabilis to improve cryopreservation knowledge. A better understanding of the benefits of cryopreservation and its widespread implementation in plant biodiversity conservation programs is required. The objective of this review is to provide a concise synthesis of the scientific contributions, current status, and applications of cryogenic techniques for the conservation of in vitro culture-derived plant tissues in Poland. First, the results contributing to research that has been achieved using cell suspensions and advances related to the use of nanoparticles and plant extracts to improve cryopreservation efficiency are discussed. Then, the applications and advances in cryopreservation of ornamental plants (roses, radiomutants, plant chimeras, Lamprocapnos spp., and geophytes), crop species (potato and garlic), forest trees, and ferns are summarized.

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Section: Solanum Tuberosum Lmentioning

confidence: 99%

Cryopreservation of Plant Tissues in Poland: Research Contributions, Current Status, and Applications

et al. 2022

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“…Cultivation of rose plants via tissue culture micropropagation has disadvantages in terms of contamination, somaclonal variations and also due to biotic stresses [1]. Hence, cryogenic storage offers a stable and safe method for long term storage of plant materials while safeguarding genetic stability [2]. Cryopreservation at temperatures of −196°C has been considered as an ideal means for long term storage of plant germplasm.…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation ofRosa hybridacv. Helmut Schmidt by PVS2 vitrification method usingin vitrofragmented explants (IFEs)

Udain

et al. 2019

Preprint

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1415 This is the first report on cryopreservation via PVS2 vitrification method on roses using in vitro 16 fragmented explants (IFEs) as the starting material. The aim of this study is to optimize the efficient 17 plant recovery and regeneration system for cryopreservation of Rosa hybrida cv. Helmut Schmidt 18 using IFEs. Some important parameters have been optimized in this study are the effect of ascorbic 19 acid (0.3 mM) examined separately and in combination at all steps in cryopreservation procedure 20 (preculture, loading, unloading and growth recovery), loading type, loading duration, and PVS2 21 duration. The highest growth recovery of 43.33% was obtained when 3-4 mm size IFEs precultured 22 on 0.25 M sucrose media supplemented with full-strength MS for one (1) day, followed by loading 23 treatment supplemented with 1.5 M glycerol + 0.4 M sucrose + 5% DMSO + 0.3 mM ascorbic acid 24 for 20 minutes, dehydration with PVS2 solution for 30 minutes and then treated with unloading 25 solution supplemented with 1.2 M sucrose + 0.3 mM ascorbic acid for 20 minutes. This finding 26 implies that long-term storage of Rosa Hybrida cv. Helmut Schmidt by PVS2 vitrification method 27 was successful with essential biomolecules. 28 29 30 Keywords: In vitro fragmented explants (IFEs), PVS2 vitrification, ascorbic acid, Biomolecules, 31 Rose 32 34 Roses are one of the most important ornamental plants in many parts of the world. This 35 is because they bloom beautiful flowers and as such, they are widely used in the horticultural 36 industry. Cultivation of rose plants via tissue culture micropropagation has disadvantages in 37 terms of contamination, somaclonal variations and also due to biotic stresses [1]. Hence, 38 cryogenic storage offers a stable and safe method for long term storage of plant materials 39 while safeguarding genetic stability [2]. Cryopreservation at temperatures of -196°C has been 40 considered as an ideal means for long term storage of plant germplasm. Through 41 cryopreservation, hybrids that are in danger of being extinct can be conserved with low 42 maintenance apart from being easy for international exchange. Cryopreservation through 43 vitrification reduces injury caused by intracellular crystallization and provides high 44 regeneration rates prior to immersion into liquid nitrogen. Basically, the most appropriate 45 plant part utilized is the meristem culture since it generates virus free lines [3]. For PVS2 46 cryopreservation study in roses, in vitro fragmented explants (IFE) represent a new source of 47 explant as starting material. IFEs were first introduced by authors [4] who conducted research 48 on Vanilla planifolia "Andrews". The study discovered that IFEs allows the production of a 49 larger number of shoots than micro cuttings after 4 months in culture. However, every 50 species of plants has different genetic makeup and respond differently to various treatments. 51 Therefore, improvement of the cryopreservation method is crucial in each step for growth 52 after recovery. Vitrification ...

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“…Therefore, the establishment of an efficient cryopreservation protocol may be a prerequisite for cryotherapy and virus eradication in tomato germplasm as well. Among the vitrification-based cryopreservation techniques, droplet vitrification produced high postcryostorage recovery (Kim and Lee, 2012) and proved to be an efficient method across many species and complex tissues, such as shoot tips and somatic or zygotic embryos (Lambardi et al, 2000;Sakai and Engelmann, 2007;Keller et al, 2008;Benelli et al, 2013). Besides the high concentration of plant vitrification solution used (Sakai et al, 1990), the droplet vitrification method is characterized by high cooling and rewarming rates (Kartha et al, 1982); this facilitates the rapid transition of intracellular water from a liquid state to a glassy state, preventing the formation of ice crystals and the irreversible damage of the cell membrane and cell components (Pearce, 2004).…”

Section: Introductionmentioning

confidence: 99%

Genetic integrity assessment of cryopreserved tomato(Lycopersicon esculentum Mill.) genotypes

Coste¹,

Şuteu²,

Băcilă³

et al. 2015

Turk J Biol

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Shoot tips of five tomato (Lycopersicon esculentum Mill.) genotypes were successfully cryopreserved by droplet vitrification. Recovered plants were studied for genetic stability by two different assays: amplified fragment length polymorphism (AFLP) using 4 primer combinations and sequencing of lycopene β-cyclase gene (LCY-B) from leaves. The highest shoot regeneration after cryopreservation was 70% (Pontica) following 24 h osmoprotection in 0.5 M sucrose and 30 min dehydration in plant vitrification solution 2 (PVS2). The data inferred from AFLP showed no genetic dissimilarities between in vitro regenerants derived from cryopreserved tissues compared with the noncryopreserved controls. Although a single nucleotide polymorphism, a G→T transversion, was identified in position 1139 in Capriciu and Darsirius, this was not due to the cryopreservation process itself, since it was detected in both cryopreserved and control samples. Thus, sequencing of LCY-B gene from leaves revealed no DNA damages after cryopreservation and subsequent in vitro regeneration. Our results indicate that cryostorage by droplet vitrification is an efficient and reliable technique to preserve the selected tomato genotypes and to regenerate true-to-type plants.

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Cryopreservation of Herbaceous Dicots

Cited by 21 publications

References 55 publications

Cryopreservation of Plant Tissues in Poland: Research Contributions, Current Status, and Applications

Cryopreservation of Plant Tissues in Poland: Research Contributions, Current Status, and Applications

Cryopreservation ofRosa hybridacv. Helmut Schmidt by PVS2 vitrification method usingin vitrofragmented explants (IFEs)

Genetic integrity assessment of cryopreserved tomato(Lycopersicon esculentum Mill.) genotypes

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