Somaclonal Variation in Populus Hybrids Regenerated from Protoplast Culture

Serres, R.; Ostry, M.E.; McCown, Brent H.; Skilling, Darroll D.

doi:10.1007/978-1-4684-7932-4_7

Cited by 6 publications

(5 citation statements)

References 4 publications

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“…Similar changes had also been observed by Serres et al (1991) in Populus hybrids regenerated from protoplast culture. Since no transformation was involved in this case, the assumption was that these were related to the in vitro culture manipulations necessary for the differentiation of transgenic plants.…”

Section: Discussionsupporting

confidence: 76%

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Poplar (Populus nigra L.) plants transformed with aBacillus thuringiensis toxin gene: insecticidal activity and genomic analysis

Wang

Castiglione

Chen

et al. 1996

Transgenic Research

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Insect-resistant poplar (Populus nigra L.) plants have been produced by infecting leaves with Agrobacterium tumefaciens strains carrying a binary vector containing different truncated forms of a Bacillus thuringiensis (B.t.)toxin gene under a duplicated CaMV 35S promoter. Putative transgenic plants were propagated by cuttings at two experimental farms (in Beijing and Xinjiang, China). At 2-3 years after transformation, 17 of them were selected on the bases of insect-tolerance and good silvicultural traits, and evaluated for insect resistance, for the presence of the B.t. toxin DNA fragment (Southern blots and PCR) and for the expression of the transgene (western and northern blots). Somaelonal variation, as suggested by the appearance of permanent changes in the shape of the leaves, was also investigated with molecular tools (RFLP (restriction fragment length polymorphism), RAPD (random amplified polymorpbic DNA) and microsatellite DNA).Bioassays with Apochemia cineraius and Lymantria dispar on the leaves of the selected clones showed different and, in some cases, high levels of insecticidal activity. The molecular analysis demonstrated integration and expression of the foreign gene. Somatic changes were correlated to extensive genomic changes and were quantified in dendrograms, in terms of genomic similarity. The analysis of control plants suggested that genomic changes were correlated to the in vitro culture step necessary for A. tumefaciens-mediated gene transfer, rather than to the integration of the foreign genes.Three transgenic clones (12, 153 and 192), selected for insect resistance, reduced morphological changes and promising silvicultural traits, are now under large-scale field evaluation in six different provinces in China.

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Section: Discussionsupporting

confidence: 76%

“…Similar somaclonal variants were also regenerated by Serres et al (1991) from in vitro cultures of the same poplar clone without transformation.…”

Section: -8819 9 1996mentioning

confidence: 80%

Poplar (Populus nigra L.) plants transformed with aBacillus thuringiensis toxin gene: insecticidal activity and genomic analysis

Wang

Castiglione

Chen

et al. 1996

Transgenic Research

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“…As shown in Table 3 of our work, the same or even more differences of stem height and diameter were observed between somaclonal variants of adventive regenerants. Different growth rates of regenerants from protoplasts of poplar were also described in work of Serres et al [29]. It may be concluded that fast-growing woody plants may be obtained due to somaclonal variability.…”

Section: Discussionmentioning

confidence: 73%

Somaclonal variations as a mean for obtaining regenerants with different growth rates in poplar (<i>Populus × berolinensis</i> Dipp.)

Gamburg¹,

Voinikov²

2013

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Adventive regenerants of Populus × berolinensis Dipp. were obtained on 1/2 MS salts with sucrose, vitamins, BA, TDZ and NAA using stem, petiole and leaf explants. They differed from each other in plantlet dimensions. More than 50 clones were produced from initial regenerants by excision and rooting of apexes and lateral shoots. Differences in stem length and thickness were observed between 200 field plants produced from in vitro plantlets. Differences in stem length were conditioned mainly due to different number of internodes and not by internodes' lengths. Plants grown from cuttings excised from highest and smallest plants retained the abilities of mother plants to grow with different rates. It is concluded that somaclonal variability can be used for selection of fast growing poplar plants which are necessary for industrial plantations. These plants can be used for this purpose without the limitations existing for transgenic plants.

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“…Although the letters Rand SC, or just S (Fry et al, 1998), have been used by different investigators for primary in-vitro regenerants, there is a need to standardize the nomenclature in this field. In that event, I would suggest that we retain the R designation for the regenerants from the somatic cells following the microshootJroot plantlet pathway, and propose the term SR for the regenerants derived from somatic cells via somatic embryogenesis, and PR (protoplast regenerants, or protoclones (Serres et al, 1991; as the primary regenerants from the protoplasts. A simplified approach would be to designate somaclones derived from any type of tissue as SC or just S. On the other hand, it might be useful to approach the problem based on the tissue of origin.…”

Section: Designation Of Somaclonal Progenymentioning

confidence: 99%

Somaclonal Variation and Induced Mutations in Crop Improvement

Jain¹,

Brar²,

Ahloowalia³

1998

Current Plant Science and Biotechnology in Agriculture

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Aims and ScopeThe book series is intended for readers ranging from advanced students to senior research scientists and corpomte directors interested in acquiring in-depth, state-of-the-art knowledge about research findings and techniques related to all aspects of agricultural biotechnology. Although the previous volumes in the series dealt with plant science and biotechnology, the aim is now to also include volumes dealing with animals science, food science and rnicrobiology. While the subject matter will relate more particularly to agricultural applications, timely topics in basic science and biotechnology will also be explored. Some volumes will report progress in rapidly advancing disciplines through proceedings of symposia and workshops while others will detail fundamental information of an enduring nature that will be referenced repeatedly.The titles published in this series are listed at the end ofthis volume.

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Somaclonal Variation in Populus Hybrids Regenerated from Protoplast Culture

Cited by 6 publications

References 4 publications

Poplar (Populus nigra L.) plants transformed with aBacillus thuringiensis toxin gene: insecticidal activity and genomic analysis

Poplar (Populus nigra L.) plants transformed with aBacillus thuringiensis toxin gene: insecticidal activity and genomic analysis

Somaclonal variations as a mean for obtaining regenerants with different growth rates in poplar (<i>Populus × berolinensis</i> Dipp.)

Somaclonal Variation and Induced Mutations in Crop Improvement

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Somaclonal Variation in Populus Hybrids Regenerated from Protoplast Culture

Cited by 6 publications

References 4 publications

Poplar (Populus nigra L.) plants transformed with aBacillus thuringiensis toxin gene: insecticidal activity and genomic analysis

Poplar (Populus nigra L.) plants transformed with aBacillus thuringiensis toxin gene: insecticidal activity and genomic analysis

Somaclonal variations as a mean for obtaining regenerants with different growth rates in poplar (&lt;i&gt;Populus &#215; berolinensis&lt;/i&gt; Dipp.)

Somaclonal Variation and Induced Mutations in Crop Improvement

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Somaclonal variations as a mean for obtaining regenerants with different growth rates in poplar (<i>Populus × berolinensis</i> Dipp.)