Somaclonal variations as a mean for obtaining regenerants with different growth rates in poplar (&amp;lt;i&amp;gt;Populus &amp;#215; berolinensis&amp;lt;/i&amp;gt; Dipp.)

Gamburg, K. Z.; Voinikov, V. K.

doi:10.4236/ns.2013.55075

Cited by 3 publications

(3 citation statements)

References 28 publications

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“…The use of cluster analysis to complement either principal coordinate or principal component analyses of morphological or genetic traits in plants is well documented (Pratta et al, 2000;Song et al, 2000;Sheidai et al, 2008Sheidai et al, , 2010Valenzuela et al, 2011;Abdellatif et al, 2012;Gamburg and Voinikov, 2013;Plazas et al, 2014). In the present study cluster analysis validated the PCOORDA plots.…”

Section: Cluster Analysissupporting

confidence: 62%

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Morphological and RAPD-marker characterization of Melia volkensii (Grke) in vitro plants regenerated via direct and indirect somatic embryogenesis

Mulanda¹,

Chuhila²,

Awori³

et al. 2015

Afr. J. Biotechnol.

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Somaclonal variation induced in vitro during tissue culture can be a problem in clonal micropropagation of elite plants. This study investigated the extent of morphological and genetic similarity or dissimilarity between Melia volkensii in vitro plants (somatic seedlings) obtained via somatic embryogenesis and normal seedlings. Comparisons were made between in vitro plants regenerated directly from cotyledon explants, indirectly from zygotic embryos and normal seedlings of the same parent trees. Regeneration was achieved using half MS medium supplemented with 0.05 mg/l thidiazuron. Shoots were elongated in half MS with 0.1 mg/l BAP plus 0.01 mg/l IAA then rooted in half MS with 0.1 mg/l IBA and 0.1 mg/l NAA. Six morphometric and five meristic characters were used for the morphological characterization. PCR-RAPD markers were used for assessment of genetic similarity or distance. Multivariate analysis using principal coordinates, cluster analysis, analysis of similarities (Anosim) and similarity percentages analysis (SIMPER) revealed significant dissimilarities (p< 0.0001) in morphometric and meristic characters between the in vitro plants and normal seedlings. However, significant similarity (p<0.01) was observed in the RAPD-genic characters of the in vitro plants and normal seedlings. Out of six morphometric traits, taproot length, internode length and shoot height were the most important sources of dissimilarity, cumulatively accounting for 72.37% of overall morphometric dissimilarity. Number of lateral roots was the single most important source of meristic dissimilarity, with 77.02% contribution. Plants regenerated directly from cotyledons were more similar to the normal seedlings in morphological and RAPD-marker characters than those regenerated indirectly from zygotic embryos. This study paves the way for identification of trait-specific RAPD markers for further characterization through sequence-characterized amplified regions (SCARs).

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Section: Cluster Analysissupporting

confidence: 62%

“…Similar approaches have been used for other tissue cultured plants such as tomato (Pratta et al, 2000), soybean (Radhakrishnan and Kumari, 2008) banana (Sheidai et al, 2008) and poplar tree (Gamburg and Voinikov, 2013).…”

Section: Introductionmentioning

confidence: 99%

Morphological and RAPD-marker characterization of Melia volkensii (Grke) in vitro plants regenerated via direct and indirect somatic embryogenesis

Mulanda¹,

Chuhila²,

Awori³

et al. 2015

Afr. J. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Although, somaclonal variation in trees is usually undesirable (Tremblay et al, 1999;Breton et al 2006;Marum et al, 2009), a few useful somaclonal variants have been reported in tree species (Ahuja, 1998;Bairu et al, 2011;Krishna et al, 2016). Some of these useful variants include: height and morphological traits in poplar (Fry et al, 1997) and olive (Leva et al, 2012); disease resistance in poplar (Ostry and Ward, 2003), apple citrus, and mango (Krishna et al, 2016); different growth rates in poplar (Gamburg and Voinikov, 2013); and stress tolerance in radiata pine (Montalbán et al, 2014).…”

Section: Micropropagationmentioning

confidence: 99%

Fate of forest tree biotechnology facing climate change

Ahuja

2021

Silvae Genetica

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Woody plants have been cultured in vitro since the 1930s. After that time much progress has been made in the culture of tissues, organs, cells, and protoplasts in tree species. Tree biotechnology has been making strides in clonal propagation by organogenesis and somatic embryogenesis. These regeneration studies have paved the way for gene transfer in forest trees. Transgenics from a number of forest tree species carrying a variety of recombinant genes that code for herbicide tolerance, pest resistance, lignin modification, increased woody bio-mass, and flowering control have been produced by Agrobacterium-mediated and biolistic methods, and some of them are undergoing confined field trials. Although relatively stable transgenic clones have been produced by genetic transformation in trees using organogenesis or somatic embryogenesis, there were also unintended unstable genetic events. In order to overcome the problems of randomness of transgene integration and instability reported in Agrobacterium-mediated or biolistically transformed plants, site-specific transgene insertion strategies involving clustered regularly interspaced short palindromic repeats (CRISPR-Cas9) platform offer prospects for precise genome editing in plants. Nevertheless, it is important to monitor phenotypic and genetic stability of clonal material, not just under greenhouse conditions, but also under natural field conditions. Genetically modified poplars have been commercialized in China, and eucalypts and loblolly pine are expected to be released for commercial deployment in USA. Clonal forestry and transgenic forestry have to cope with rapid global climate changes in the future. Climate change is impacting species distributions and is a significant threat to biodiversity. Therefore, it is important to deploy Strategies that will assist the survival and evolution of forest tree species facing rapid climate change. Assisted migration (managed relocation) and biotechnological approaches offer prospects for adaptation of forest trees to climate change.

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Assessment of direct shoot organogenesis and genetic fidelity in Solanum viarum Dunal—a commercially important medicinal plant

Pandey

Patel

Prasad

et al. 2020

In Vitro Cell.Dev.Biol.-Plant

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Somaclonal variations as a mean for obtaining regenerants with different growth rates in poplar (<i>Populus × berolinensis</i> Dipp.)

Cited by 3 publications

References 28 publications

Morphological and RAPD-marker characterization of Melia volkensii (Grke) in vitro plants regenerated via direct and indirect somatic embryogenesis

Morphological and RAPD-marker characterization of Melia volkensii (Grke) in vitro plants regenerated via direct and indirect somatic embryogenesis

Fate of forest tree biotechnology facing climate change

Assessment of direct shoot organogenesis and genetic fidelity in Solanum viarum Dunal—a commercially important medicinal plant

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