Somaclonal variability of Ungernia victoris: the necessity of comprehensive genetic analysis

Abstract

An analysis of five 10-year-old U. victoris cell lines of common origin revealed mixoploidy, significant level of cell polyploidization, quantitative and qualitative changes in individual RAPD-fragments. No regularity was observed in the lines clustering depending on the nutrient medium phytohormone composition or genealogy of the lines. The cytogenetic, molecular and genetic approaches are recommended to ensure reliable determination of somaclonal changes, because the variations in ploidy levels do not always result in the RAPD-polymorphism.

Genetic Variation Induced by Tissue and Organ Culture in Gentiana Species

“…9.8 and 9.11). On the whole, our results are consistent with the data available in the literature, indicating that most genome rearrangements occur in the course of dedifferentiation during the early steps of culture in the formation of a stable cell population in vitro (Kunakh 2005;Kuznetsova et al 2005;Bublyk et al 2008;Andreev et al 2009). It is known that an increase in number of passages and their duration may increase the occurrence and rate of somaclonal variation in the callus (Bairu et al 2011).…”

“…According to other data, the level of genetic changes during the early stages of culture may be low but then increase with the duration of culture. For example, using RAPD-PCR and ISSR-PCR, it was demonstrated that long-term culture of Ungernia victoris may result in increased genetic distances between donor plant and lines of callus, as well as divergence of the latter (Bublyk et al 2008(Bublyk et al , 2010.…”

“…In particular, it is known that regenerants derived through direct organogenesis exhibit less genetic variation than plants regenerated via tissue culture (Kunakh 2005). A major reason for increase in somaclonal variation upon indirect regeneration is considered to be the increased genetic heterogeneity of cultured cells due to accumulation of genetic changes as a result of long-term culture (Kunakh 2005;Bublyk et al 2008). However, the value of the genetic distance from the donor plant in the G. pneumonanthe regenerant (K 2 genotype) derived through indirect organogenesis was somewhat less compared with the 1-year-old morphogenic culture from which it was obtained.…”

The Gentianaceae - Volume 2: Biotechnology and Applications

“…On the whole, our results are consistent with the data available in the literature, indicating that most genome rearrangements occur in the course of dedifferentiation during the early steps of culture in the formation of a stable cell population in vitro Kuznetsova et al 2005;Bublyk et al 2008;Andreev et al 2009). In particular, PCR analysis showed that callus of G. pneumonanthe and G. lutea underwent detectable changes during the early stages (prior to 6 months).…”

“…In particular, PCR analysis showed that callus of G. pneumonanthe and G. lutea underwent detectable changes during the early stages (prior to 6 months). For example, using RAPD-PCR and ISSR-PCR, it was demonstrated that long-term culture of Ungernia victoris may result in increased genetic distances between donor plant and lines of callus, as well as divergence of the latter (Bublyk et al 2008(Bublyk et al , 2010). 9.8 and 9.11).…”