Genetic variability in regenerated plants of Ungernia victoris

Bublyk, O. M.; Андреев, И. О.; Spiridonova, K. V.; Кунах, В. А.

doi:10.1007/s10535-011-0220-6

Cited by 3 publications

(3 citation statements)

References 24 publications

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“…This feature of cultured cells led to almost collapse of aspirations and hopes that were pinned on the culture of isolated cells and tissues as an inexhaustible source of new plant forms with previously unknown characteristics valuable for genetics and plant breeding. Today, you can count on one hand somaclonal variants with fundamentally new features (evidence and references, see [2,4,6,7,11,12,22,23,[68][69][70][71].…”

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confidence: 99%

Evolution of cell populations in vitro: peculiarities, driving forces, mechanisms and consequences

Кунах

2013

Biopolym. Cell

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This review outlines the major features and distinctions of cell populations, types and directions of selection in such populations. Population-genetic basis for cell adaptation to growth conditions in vitro is elucidated; in particular, peculiarities of genome evolution in the course of cell dedifferentiation and further cell adaptation to growth conditions in passaged culture are evaluated. Main factors of variation and selection in cell populations in vitro, influence of growth conditions on structure of cell populations and some regularities of cultured cells and regenerated plants are considered. Details of creation of stable cell lines-producers of biologically active substances are presented. Views and suppositions of author resulting from analysis of both literature data and own multiyear studies on cell population genetics are set forth. Among others are substantiated such key statements: cell culture in vitro presents dynamically-heterogeneous biological system, clone population, which is developing (evolving) as a result of major driving factors of evolution – variation, heredity, selection and drift of genes (genotypes); interaction between these processes determines the biological characteristics of each particular cell line grown in specific conditions; in adaptation of cells to growth conditions in vitro one can single out three periods: the initial population of isolated cells, the period of strain (cell line) formation and the established strain. The division into periods is determined by the type, direction and intensity of «natural» selection that acts in cell population. The formed (adapted to growth in vitro) strains are genetically heterogeneous, they are characterized by the presence of physiological and genetic homeostasis, which are mostly caused by the action of stabilizing selection; cultured cells of higher plants are able to synthesize practically all classes of secondary (specialized) compounds (alkaloids, steroids, terpenoids, etc.); any somatic cell with living (functionally active) nucleus during its isolation and further cultivation in tissue culture, as a result of the process of «somaclonal» variability occurring according to the N. I. Vavilov’s law of homologous series in hereditary variability, can restore in it’s descendants, including regenerated plants, the entire genetic polymorphism (or at least a significant part of it) characteristic of the plant’s species and may be even it’s genus. This provides an opportunity to preserve and restore the natural polymorphism in cultured cells and tissues in vitro

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confidence: 99%

Evolution of cell populations in vitro: peculiarities, driving forces, mechanisms and consequences

Кунах

2013

Biopolym. Cell

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“…На сьогодні сомаклональних варіантів з принципово новими ознаками отримано одиниці (підтвердження і посилання див. [67,92,93,108,159,160,163,[168][169][170][171][172][173][174]).…”

Section: деякі закономірності мінливості культивованих клітин і рослин-регенерантівunclassified

Cell population genetics: emergence, main results and concepts (to the 50th anniversary of the foundation)

Кунах¹

2018

Visn. ukr. tov. genet. sel.

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The article briefly reviews the emergence and development in Ukraine of a new scientific field — cell population genetics, which formed the theoretical basis of modern cell technologies. These include, in particular, plant biotechnologies for the improvement, preservation, and accelerated reproduction of unique genotypes in vitro; development of new genotypes (organisms) by the methods of cell and genetic engineering and cellular selection; obtaining biologically active compounds, including recombinant, from biomass of cultured cells and tissues for the needs of medicine, cosmetics and food industry; as well as the methods of cell therapy, including technologies based on the use of stem cells, etc. Cultured cells are widely used as model objects and biological systems for studying the most relevant problems of modern biology: the features of the course, signaling pathways, and mechanisms of cell proliferation, including carcinogenesis and tumor proliferation; dedifferentiation of cells, including their reversion to a pluripotent state; totipotency, pluripotency and omnipotency; regeneration of tissues, separate organs, and whole organisms, etc. The article reviews scientific prerequisites for the development of the new scientific field and presents the main concepts of cell population genetics, which have been mainly developed in the Department of cell population genetics of the Institute of Molecular Biology and Genetics of the National Academy of Sciences of Ukraine. It also presents an analysis of the features of development of the newest research areas of genetics of somatic cells of intact plants and cells in vitro, cell population genetics, genetic foundations of cell selection, cell biology, and biotechnology during the second half of the past and the beginning of this century. Keywords: history of science, cell population genetics, plant tissue and cell culture, cell selection, plant biotechnology.

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“…Both ways of morphogenesis can occur with an intermediate stage of callus formation (indirect regeneration) or without it (direct regeneration). Direct regeneration is the pre ferred way of morphogenesis for the reproduction of rare and endangered species, allowing to avoid the somaclonal variation and to preserve the identity of obtained clones to the mother plant (Varshney et al, 2001;Bublyk et al, 2012;Yin et al, 2013).…”

Section: Fritillaria Sonnikovae Schaulo Et a Erst Familymentioning

confidence: 99%

In vitro regeneration from bulbous scales of Fritillaria sonnikovae, an endemic species

2015

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Features of in vitro regeneration of Fritillaria sonnikovae from bulb scales were studied. The initi ation of shoot formation was obtained on a nutrient medium BDS, supplemented with 5 μM 6 benzylami nopurine and 2 μM α naphthaleneacetic acid. Optimization of propagation stage was carried out using obtained microbulbs as explants. High regenerative response of explants and shoot multiplication rate were observed on both media supplemented with growth regulators (up to 47% and 4.2 ± 0.6 shoots per explant, respectively) and hormone free medium (48% and 4.1 ± 0.2 shoots per explant, respectively). It has been established that the addition of growth regulators on the stage of cultivation does not cause increased mor phogenic response but contributes to the accelerated initiation and development of microbulbs. Morpholog ical and histological analysis revealed the dynamics of the formation of shoots de novo. In vitro development of F. sonnikovae follows the path of direct organogenesis from the epidermal tissue of the explant.

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Genetic variability in regenerated plants of Ungernia victoris

Cited by 3 publications

References 24 publications

Evolution of cell populations in vitro: peculiarities, driving forces, mechanisms and consequences

Evolution of cell populations in vitro: peculiarities, driving forces, mechanisms and consequences

Cell population genetics: emergence, main results and concepts (to the 50th anniversary of the foundation)

In vitro regeneration from bulbous scales of Fritillaria sonnikovae, an endemic species

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