In vitro regeneration from bulbous scales of Fritillaria sonnikovae, an endemic species

Abstract

Features of in vitro regeneration of Fritillaria sonnikovae from bulb scales were studied. The initi ation of shoot formation was obtained on a nutrient medium BDS, supplemented with 5 μM 6 benzylami nopurine and 2 μM α naphthaleneacetic acid. Optimization of propagation stage was carried out using obtained microbulbs as explants. High regenerative response of explants and shoot multiplication rate were observed on both media supplemented with growth regulators (up to 47% and 4.2 ± 0.6 shoots per explant, respectively) and hormone free medium (48% and 4.1 ± 0.2 shoots per explant, respectively). It has been established that the addition of growth regulators on the stage of cultivation does not cause increased mor phogenic response but contributes to the accelerated initiation and development of microbulbs. Morpholog ical and histological analysis revealed the dynamics of the formation of shoots de novo. In vitro development of F. sonnikovae follows the path of direct organogenesis from the epidermal tissue of the explant.

Establishment of Artificial Rapid Propagation System of Fritillaria crassicaulis

“…It is speculated that after meristem is formed, 2, 4-D cumulating to some level will restrain relevant protein synthesis in callus and stop the further redifferentiation. Some other research indicates that NAA is the effective auxin to promote scales to become bulblets (Jin et al 2014;Kulkhanova et al 2015), which is verified by the 6-BA/NAA full-factorial experiment: NAA drastically promoted the effect of 6-BA on the induction of bulblets, and the calli were completely restricted as long as the bulblets emerged. In the successive three-factor orthogonal experiment, the regenerated callus induction frequency rose significantly and gave rise to the consequence that proliferation coefficient increased sharply, a fact that indicates that the coordinative role from multifactors presented evident effect on the proliferation culture on regenerated bulblets of F. crassicaulis.…”

“…For example, organs, such as scale leaf, root, and bulb base, of plants in Fritillaria and Lilium have the capability to dedifferentiate to become meristems, which enriches the origin of explants (Jura-Morawiec et al 2015). Almost all reports on in vitro rapid propagation of plants in Fritillaria and Lilium introduce patterns as follows: explant-callus-adventitious bud-bulblet-rooting plantlet (Jin et al 2014;Muraseva and Novikova 2018;Saeed and Bahattin 2020;Skori c et al 2012), explant-adventitious bud-bulblet-rooting plantlet (Andola et al 2007;Kulkhanova et al 2015;Muraseva et al 2015), explantcallus-embryoid body-bulblet-rooting plantlet (Du et al 2014;Marija et al 2011), or explant-embryoid body-bulblet-rooting plantlet (Pelkonen and Kauppi 1999;Tribulato et al 1997). Every pattern fulfills rapid propagation via the emergence of a bulblet that stems from three origins: callus, adventitious bud, or embryoid body.…”

In vitro propagation of Eranthis stellata (Ranunculacea), endemic species with narrow distribution in the Russian Far East, Northeast China and North Korea

“…For example, BAP was successfully used for in vitro propagation of Gladiolus species and varieties (Hus sey 1977; Akhare et al 2008), and BAP in combination with auxins was used to propagate Muscari mirum Speta (Nasircilar et al 2011), Iris stenophylla Hausskn & Siehe ex Baker subsp. allisonii B. Mathew (Nasırcılar et al 2011), and species of the genus Fritillaria (Kulkhanova et al 2015, Muraseva et al 2015, Muraseva & Novikova 2018.…”