Solving multicomponent chiral separation challenges using a new SFC tandem column screening tool

Abstract

A tool for improved tandem column chiral supercritical fluid chromatography (SFC) method development screening was prepared by modification of a commercial analytical SFC instrument with two different software-controllable, six position high-pressure column selection valves, each controlling a bank of five different columns and a pass through line. The resulting instrument, which has the ability to screen 10 different individual columns and 25 different tandem column arrangements, is a useful tool for facilitating the screening of tandem column SFC arrangements for separation of complex mixtures of stereoisomers or other multicomponent mixtures. Strategies for optimal use of the instrument are discussed, and several examples of the use of the instrument in developing tandem SFC methods for resolution of multicomponent mixtures are presented.

Chiral Recognition Mechanism: Practical Considerations for Pharmaceutical Analysis of Chiral Compounds

“…First, a wider range of CSPs will be tested, but derivatization of targeted molecule and column coupling can also be envisaged. Recently the SFC coupling of two different chiral columns in series has been reported for the resolution of the four stereoisomers of pharmaceutical compounds containing two chiral centers [168,169].…”

Chiral separations in sub‐ and supercritical fluid chromatography

“…[49]. Two arrays of five chiral columns are used to screen chiral molecules using a standard gradient method (4% MeOH during 4 min, the ramp at 2%/min to 40% MeOH and hold for 3 min, 200 bar, 358C, 1.5 mL/ min, analysis time 25 min).…”

Supercritical Fluid Chromatography

“…An effective screening system is the key for chiral method development. Welch et al (2007) reported a chiral SFC tandem column screening system. With modifi cation of a commercial analytical SFC instrument, two banks of fi ve different columns were individually controlled with two six -position high -pressure column selection valves.…”